FlyBase Allele Report: Dmel\nanos[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\nanos^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0322536

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

P{UAS-nanos.RNAi.WIZ}

Also Known As

UAS-nosRNAi

Key Links

Transgenic product class

RNAi_reagent

(Menon et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Menon et al., 2009)

Mutagen

in vitro construct

(Menon et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-nanos.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of nos sequence (nucleotides 744-1203 in exon 2). The repeats are in tail-to-tail orientation and are separated by a w intron.

(Menon et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

nanos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Menon et al., 2009)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

(Menon et al., 2009)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Mhc.PU

(Menon et al., 2009)

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

dendrite | third instar larval stage, with Scer\GAL4^ppk.1.9

(Bhogal et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mhc.PU

(Menon et al., 2009)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.1.9

(Bhogal et al., 2016)

mitochondrion | third instar larval stage, with Scer\GAL4^ppk.1.9

(Bhogal et al., 2016)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Menon et al., 2009)

NMJ bouton | third instar larval stage, with Scer\GAL4^how-24B

(Menon et al., 2009)

presynaptic active zone | third instar larval stage, with Scer\GAL4^elav-C155

(Menon et al., 2009)

Detailed Description

Statement

Reference

Expression of nos^{dsRNA.Scer\UAS.WIZ} RNAi under the control of Scer\GAL4⁴⁷⁷ results in dendritic field coverage defects and loss of terminal branches in class IV dendritic arborizing (da) neurons in third instar larvae due to net loss rather than gain of terminal branches during mid-to-late third larval instar. nos^{dsRNA.Scer\UAS.WIZ} RNAi expressing neurons display perturbed branch dynamics as the retraction rates are highly increased. Expression under the Scer\GAL4^ppk.1.9 driver leads to a significantly increased density of mitochondria in dendrites compared to controls.

(Bhogal et al., 2016)

Expression of nos^{dsRNA.Scer\UAS.WIZ} driven by Scer\GAL4^elav-C155 or Scer\GAL4^how-24B results in a significant increase in total bouton number at the third instar larval neuromuscular junction, compared to controls; the number of active zones in type 1s boutons is significantly increased with expression driven by Scer\GAL4^elav-C155. Expression of nos^{dsRNA.Scer\UAS.WIZ} driven by Scer\GAL4^Mhc.PU results in a significant decrease in GluRIIA (and increase in GluRIIB) immunoreactivity at NMJ boutons. Recordings from the NMJ of Scer\GAL4^Mhc.PU>nos^{dsRNA.Scer\UAS.WIZ} larvae show a significant decrease in mEJP (mini) amplitude and faster mEJP decay times, compared to controls.

(Menon et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Df(3L)H99/+

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^GD1673/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Df(3L)grim^C15E/rpr[+]/rpr⁸⁷/+

(Bhogal et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4^ppk.1.9, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4^ppk.1.9, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4^ppk.1.9, nanos^RNAi.UAS.WIZ has mitochondrion | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by Df(3L)H99/+

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Df(3L)H99/+

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^GD1673/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, suppressible by hid^GD1673/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has larval multidendritic class IV neuron | third instar larval stage phenotype, non-suppressible by Df(3L)grim^C15E/rpr[+]/rpr⁸⁷/+

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ has dendrite | third instar larval stage phenotype, non-suppressible by Df(3L)grim^C15E/rpr[+]/rpr⁸⁷/+

(Bhogal et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic field coverage defects and loss of terminal branches observed in class IV dendritic arborizing neurons of third instar larvae expressing nos^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4⁴⁷⁷ is suppressed by combination with a single copy of hid^A206 or Df(3L)H99 or by co-expression of hid^GD1673 RNAi or Diap1^Scer\UAS.cHa but not by combination with both Df(3L)grim^C15E and rpr⁸⁷ (one copy each). The increased branch retraction rate characteristic for nos^{dsRNA.Scer\UAS.WIZ} RNAi (driven by Scer\GAL4⁴⁷⁷) expressing larvae as well as the increased density of mitochondria in dendrites (RNAi driven by Scer\GAL4^ppk.1.9) are also rescued by combination with hid^A206.

(Bhogal et al., 2016)

Xenogenetic Interactions

Statement

Reference

(Bhogal et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

nanos^RNAi.UAS.WIZ

nanos^{dsRNA.UAS.WIZ}

nos^{dsRNA.Scer\UAS.WIZ}

nos^{dsRNA.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (4)

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