FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4p65.Met.CH321-28O05
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General Information
Symbol
Scer\GAL4p65.Met.CH321-28O05
Species
S. cerevisiae
Name
FlyBase ID
FBal0328042
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Met-GAL4::p65}
Also Known As
Met-GAL4::p65
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Baumann et al., 2017)
Progenitor genotype
Carried in construct
P{Met-GAL4::p65}
Cytology
Description

The GAL4::p65 driver is expressed under the control of Met regulatory sequences. Consists of the genomic BAC clone CH321-28O05 in which the first exon of Met has been replaced with a GAL4::p65 driver followed by expression termination sequences. There is a small deletion in sequence upstream of the Met promoter (within the upstream CG1703 gene).

(Baumann et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
Met
Encoded product / tool
GAL4::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      second instar larval stage -- prepupal stage P1
      embryonic/larval corpus allatum
      (Baumann et al., 2017)
      embryonic/larval corpus cardiacum
      (Baumann et al., 2017)
      prothoracic gland
      (Baumann et al., 2017)
      wandering third instar larval stage
      embryonic/larval epidermis
      (Baumann et al., 2017)
      histoblast
      (Baumann et al., 2017)
      embryonic/larval oenocyte
      (Baumann et al., 2017)
      larval somatic muscle cell
      (Baumann et al., 2017)
      embryonic/larval fat body
      (Baumann et al., 2017)
      embryonic/larval salivary gland
      (Baumann et al., 2017)
      larval central nervous system | restricted
      (Baumann et al., 2017)
      embryonic/larval mushroom body | intense
      (Baumann et al., 2017)
      medulla anlage
      (Baumann et al., 2017)
      lobula anlage
      (Baumann et al., 2017)
      lobula plate anlage
      (Baumann et al., 2017)
      larval PTTH neuron of pars lateralis
      (Baumann et al., 2017)
      larval corpus allatum innervating neuron CA-LP 1
      (Baumann et al., 2017)
      larval corpus allatum innervating neuron CA-LP 2
      (Baumann et al., 2017)
      photoreceptor neuron of eye disc
      (Baumann et al., 2017)
      genital disc
      (Baumann et al., 2017)
      wing disc
      (Baumann et al., 2017)
      prothoracic leg disc
      (Baumann et al., 2017)
      mesothoracic leg disc
      (Baumann et al., 2017)
      adult stage | male
      ejaculatory duct
      (Baumann et al., 2017)
      ejaculatory bulb
      (Baumann et al., 2017)
      male accessory gland
      (Baumann et al., 2017)
      vas deferens
      (Baumann et al., 2017)
      adult stage | female
      spermatheca | intense
      (Baumann et al., 2017)
      seminal receptacle
      (Baumann et al., 2017)
      uterus
      (Baumann et al., 2017)
      adult stage
      main body follicle cell | intense
      (Baumann et al., 2017)
      adult brain | restricted
      (Baumann et al., 2017)
      adult mushroom body | intense
      (Baumann et al., 2017)
      adult pharyngeal nerve
      (Baumann et al., 2017)
      peptidergic neuron | faint
      (Baumann et al., 2017)
      ellipsoid body
      (Baumann et al., 2017)
      fan-shaped body

      Comment: layers

      (Baumann et al., 2017)
      bulb
      (Baumann et al., 2017)
      nodulus 3 | intense
      (Baumann et al., 2017)
      nodulus 2
      (Baumann et al., 2017)
      nodulus 1
      (Baumann et al., 2017)
      lobula complex
      (Baumann et al., 2017)
      medulla
      (Baumann et al., 2017)
      lobula layer 1 | intense
      (Baumann et al., 2017)
      T5 neuron
      (Baumann et al., 2017)
      T4 neuron
      (Baumann et al., 2017)
      second optic chiasma
      (Baumann et al., 2017)
      RT-PCR
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      wandering third instar larval stage
      wing disc
      (Baumann et al., 2017)
      metathoracic leg disc
      (Baumann et al., 2017)
      larval central nervous system
      (Baumann et al., 2017)
      Additional Information
      Statement
      Reference

      ScerGAL4p65.Met.CH321-28O05 drives strong expression in the adult mushroom bodies and is widely expressed across the compartments of the central complex. It is highly expressed in nodulus subunit III compared to the other subunits. It is also expressed across the optic lobe neuropils and has strong expression in the aborizations of T5 neurons in lobula layer 1.

      (Baumann et al., 2017)
       
      Marker for
      Reflects expression of
      Met
      (Baumann et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4Met.CH321-28O05.T:Hsap\RELA
      Scer\GAL4p65.Met.CH321-28O05
      Name Synonyms
      Secondary FlyBase IDs
        References (2)