FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\TfIIFα
Open Close
General Information
Symbol
Dmel\TfIIFα
Species
D. melanogaster
Name
Transcription factor IIFα
Annotation Symbol
CG10281
Feature Type
protein_coding_gene
FlyBase ID
FBgn0010282
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. (UniProt, Q05913)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

TFIIF

Key Links
Genomic Location
Cytogenetic map
83E5-83E5
Sequence location
Recombination map
3-47.5
RefSeq locus
NT_033777 REGION:6380467..6382604
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables TFIIF-class transcription factor complex binding
inferred from physical interaction with FLYBASE:TfIIFβ; FB:FBgn0010421
(Gong et al., 1995)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables DNA binding
inferred from electronic annotation with InterPro:IPR008851
(InterPro Project Members, 2004-)
enables RNA polymerase II general transcription initiation factor activity
inferred from biological aspect of ancestor with PANTHER:PTN000313913
(GO Reference Genome Project, 2011-)
enables TFIIF-class transcription factor complex binding
inferred from biological aspect of ancestor with PANTHER:PTN000313913
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in transcription by RNA polymerase II
inferred from direct assay
(Gong et al., 1995)
involved_in transcription elongation by RNA polymerase II
inferred from direct assay
(Kephart et al., 1994)
involved_in transcription initiation at RNA polymerase II promoter
inferred from direct assay
(Kephart et al., 1994)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in positive regulation of transcription elongation by RNA polymerase II
inferred from electronic annotation with InterPro:IPR008851
(InterPro Project Members, 2004-)
involved_in transcription initiation at RNA polymerase II promoter
inferred from biological aspect of ancestor with PANTHER:PTN000313913
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR008851, InterPro:IPR011039
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with SGD:S000003418
(Aoyagi and Wassarman, 2000)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Gong et al., 1995)
part_of transcription factor TFIIF complex
inferred from direct assay
(Austin and Biggin, 1996)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in nucleus
inferred from electronic annotation with InterPro:IPR008851
(InterPro Project Members, 2004-)
part_of transcription factor TFIIF complex
inferred from biological aspect of ancestor with PANTHER:PTN000313913
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with SGD:S000003418
(Aoyagi and Wassarman, 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TFIIF alpha subunit family. (Q05913)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
TRANSCRIPTION FACTOR II F -
The general transcription factor complex Transcription factor II F (TFIIF) prevents non-specific interaction of RNA Pol II with DNA and stabilizes the pre-initiation complex (PIC), in particular stabilizing TFIIB within the PIC. (Adapted from PMID:25693126).
Protein Function (UniProtKB)
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
(UniProt, Q05913)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\TfIIFα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q05913)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
TfIIFα-RA
FBtr0081719
NM_079522
1938
577
Additional Transcript Data and Comments
Reported size (kB)

1.929 (longest cDNA)

(Kephart et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
TfIIFα-PA
FBpp0081216
64.5
577
8.45
Q05913
NP_524246
AAF54125
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

577 (aa)

(Gong et al., 1993, Kephart et al., 1993)
Comments
External Data
Subunit Structure (UniProtKB)

Heterodimer of an alpha and a beta subunit.

(UniProt, Q05913)
Post Translational Modification

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.

(UniProt, Q05913)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TfIIFα using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.72

Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
(Arbeitman et al., 2004)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
anlage in statu nascendi
(Fisher et al., 2012)
anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
central brain anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
foregut anlage in statu nascendi
(Fisher et al., 2012)
head mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hindgut anlage in statu nascendi
(Fisher et al., 2012)
mesectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
trunk mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
anterior endoderm anlage
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
foregut anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
mesectoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
amnioserosa
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
amnioserosa
(Fisher et al., 2012)
anterior midgut primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic proventriculus
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval salivary gland
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
germline cell
(Arbeitman et al., 2004)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Gong et al., 1995)
part_of transcription factor TFIIF complex
inferred from direct assay
(Austin and Biggin, 1996)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\TfIIFα in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of TfIIFα
Transgenic constructs containing regulatory region of TfIIFα
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 10 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\GTF2F1
13 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Gtf2f1
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Gtf2f1
12 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\gtf2f1
10 of 13
Yes
Yes
Xtro\otogl2
1 of 13
No
No
Danio rerio (Zebrafish) (1)
Drer\gtf2f1
12 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\gtf-2F1
11 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP002779
11 of 12
Yes
Yes
Agam\AgaP_AGAP000029
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\RAP74
6 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\TFG1
4 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\tfg1
3 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:TfIIFα. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    GTF2F1; general transcription factor IIF subunit 1
    13 of 14
    189968
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Heterodimer of an alpha and a beta subunit.
        (UniProt, Q05913 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-47.5
        Cytogenetic map
        83E5-83E5
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        83E5-83E5
        Limits computationally determined from genome sequence between P{lacW}casj1C2 and P{PZ}lab01241
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-47.5

        (FlyBase, 2016)

        3-44.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (79)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Kephart et al., 1993)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              TfIIF has been purified and shown to include two subunits.

              (Austin and Biggin, 1996)

              Analysis of TfIIFα deletion constructs in vitro suggests that almost all of the protein is required for transcription initiation, while only the N-terminal region is required for stimulation of transcription elongation.

              (Kephart et al., 1994)

              Drosophila TfIIFα cloned by low-stringency hybridisation to human TfIIFα cDNA.

              (Gong et al., 1993)

              Cloned by sequence similarity to human RAP74 protein.

              (Kephart et al., 1993)
              Relationship to Other Genes
              Source for database merge of
              Additional comments
              Nomenclature History
              Source for database identify of

              Source for identity of: TfIIFα CG10281

              (Wang, 1999.11)
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (17)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 57 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              References (71)