FlyBase Gene Report: Dmel\RpL3

General Information

Symbol

Dmel\RpL3

Species

D. melanogaster

Name

Ribosomal protein L3

Annotation Symbol

CG4863

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020910

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

The L3 protein is a component of the large subunit of cytoplasmic ribosomes. (UniProt, O16797)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Key Links

Genomic Location

Cytogenetic map

86D8-86D8

Sequence location

3R:11,221,894..11,225,173 [+]

Recombination map

3-50

RefSeq locus

NT_033777 REGION:11221894..11225173

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables RNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000146039

(GO Reference Genome Project, 2011-)

enables structural constituent of ribosome

traceable author statement

(Marygold et al., 2007)

inferred from electronic annotation with InterPro:IPR000597, InterPro:IPR045077

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000146039

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

traceable author statement

(Marygold et al., 2007)

inferred by curator from GO:0022626

(Anger et al., 2013)

involved_in translation

inferred from electronic annotation with InterPro:IPR000597, InterPro:IPR045077

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000146039

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of cytosolic large ribosomal subunit

inferred from biological aspect of ancestor with PANTHER:PTN000146039

(GO Reference Genome Project, 2011-)

traceable author statement

(Marygold et al., 2007)

located_in ribosome

inferred from electronic annotation with InterPro:IPR000597, InterPro:IPR045077

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

CYTOPLASMIC LARGE RIBOSOMAL SUBUNIT

CYTOPLASMIC LARGE RIBOSOMAL SUBUNIT - TESTIS-SPECIFIC VARIANT

Protein Family (UniProt)

Belongs to the universal ribosomal protein uL3 family. (O16797)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC LARGE RIBOSOMAL SUBUNIT -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytosol. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the large subunit is to bind tRNA during the synthesis of polypeptides. (Adapted from FBrf0205398.)
CYTOPLASMIC LARGE RIBOSOMAL SUBUNIT - TESTIS-SPECIFIC VARIANT -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytosol. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the large subunit is to bind tRNA during the synthesis of polypeptides. Tissue expression data from modENCODE has been used to infer the composition of the cytosolic large ribosomal subunit testis-specific variant. (Adapted from FBrf0205398.)

Protein Function (UniProtKB)

The L3 protein is a component of the large subunit of cytoplasmic ribosomes.

(UniProt, O16797)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpL3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O16797)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpL3-RA

FBtr0082346

NM_079592

1381

416

RpL3-RD

FBtr0082347

NM_169377

715

161

RpL3-RG

FBtr0302586

NM_169380

1688

138

RpL3-RH

FBtr0336783

NM_169378

1610

416

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Chan et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RpL3-PA

FBpp0081822

46.9

416

10.95

FBpp0081823

18.1

161

10.28

NP_731547

AAF54612

RpL3-PG

FBpp0291742

15.6

138

10.80

NP_731550

AAF54611

RpL3-PH

FBpp0307759

46.9

416

10.95

O16797

NP_731548

AAN13496

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

416 aa isoforms: RpL3-PA, RpL3-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

416 (aa); 47 (kD predicted)

(Chan et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O16797

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpL3 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.81

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval hindgut

(Chan et al., 1998)

organism | ubiquitous

(Chan et al., 1998)

larval muscle system

(Chan et al., 1998)

embryonic/larval midgut

(Chan et al., 1998)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

clypeo-labral primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

rectum primordium

Comment: reported as rectum specific anlage

(Fisher et al., 2012)

small intestine primordium

Comment: reported as small intestine specific anlage

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

Additional Descriptive Data

RpL3 transcripts are ubiquitously expressed in embryos. At later embryonic stages, higher transcript levels are observed in the midgut, hindgut, and muscle.

(Chan et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RpL3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpL3

View exonic expression by tissue for Dmel\RpL3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020910

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41347/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020910

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020910

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020910

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4863

Flygut - An atlas of the Drosophila adult midgut

FBgn0020910

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\RpL3

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpL3

Transgenic constructs containing regulatory region of RpL3

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(3R)ED5511

(Marygold et al., 2007)

Df(3R)ED5514

(Marygold et al., 2007, Cook, 2006.10.1)

Df(3R)ED5518

(Marygold et al., 2007, Cook, 2006.10.1)

Df(3R)M86D

(Marygold et al., 2007)

Df(3R)MS32

(Marygold et al., 2007)

Gene not disrupted in

Df(3R)ED5516

(Ryder and Roote, 2004.11.10)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

RpL3^GD13494

lethal, with Scer\GAL4^pnr-MD237

RpL3^GD13494

lethal, with Scer\GAL4^tin.CΔ4

RpL3^GD13494

lethal | pupal stage, with Scer\GAL4^pnr-MD237

RpL3^GD13494

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL3^GD13494

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL3^GD16490

viable, with Scer\GAL4^pnr-MD237

RpL3^GD16490

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL3^GD13494

some die during larval stage, with Scer\GAL4^tin.CΔ4

RpL3^GD13494

Phenotype manifest in

Allele

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

RpL3^GD13494

sarcomere, with Scer\GAL4^Mef2.PR

RpL3^GD13494

Z disc, with Scer\GAL4^Mef2.PR

RpL3^GD13494

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\RPL3

14 of 14

Yes

Hsap\RPL3L

12 of 14

Yes

Hsap\MRPL3

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Rpl3

14 of 14

Yes

Rnor\Rpl3l

11 of 14

Yes

Rnor\Mrpl3

1 of 14

Rnor\Nme4

1 of 14

Yes

Mus musculus (laboratory mouse) (3)

Mmus\Rpl3

13 of 14

Yes

Mmus\Rpl3l

11 of 14

Yes

Mmus\Mrpl3

2 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\rpl3l

9 of 13

Yes

Xtro\rpl3

8 of 13

Yes

Xtro\LOC101732800

1 of 13

Yes

Xtro\pold3

1 of 13

Yes

Xtro\rps27a

1 of 13

Danio rerio (Zebrafish) (2)

Drer\rpl3

14 of 14

Yes

Drer\mrpl3

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\rpl-3

13 of 14

Yes

Cele\mrps-18C

1 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP003556

11 of 12

Yes

Agam\AgaP_AGAP000857

1 of 12

Arabidopsis thaliana (thale-cress) (4)

Atha\RPL3B

13 of 13

Yes

Atha\RP1

12 of 13

Yes

Atha\AT2G43030

1 of 13

Atha\RPL3P

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RPL3

13 of 13

Yes

Scer\MRPL9

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\rpl301

12 of 12

Yes

Spom\rpl302

12 of 12

Yes

Spom\mrpl9

2 of 12

Escherichia coli (enterobacterium) (1)

Ecol\rplC

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RpL3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

mRpL3

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

RPL3L; ribosomal protein L3 like

model of dilated cardiomyopathy 2D

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPL3; ribosomal protein L3

14 of 14

604163

RPL3L; ribosomal protein L3 like

12 of 14

617416

CARDIOMYOPATHY, DILATED, 2D; CMD2D

dilated cardiomyopathy 2D

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpL3 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpL3 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy, anti tag coimmunoprecipitation, western blot

(Villa et al., 2024, Anger et al., 2013)

RpL3 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL37-1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy, anti tag coimmunoprecipitation, western blot

(Villa et al., 2024, Anger et al., 2013)

RpL3 - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL3 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpL3 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL3 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66481

DroID - A comprehensive database of gene and protein interactions.

FBgn0020910

MIST (protein-protein) - An integrated Molecular Interaction Database

41347

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-50

Cytogenetic map

86D8-86D8

Sequence location

3R:11,221,894..11,225,173 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86D8-86D8

Limits computationally determined from genome sequence between P{EP}EP3340^EP3340 and P{PZ}tho¹&P{PZ}l(3)04629⁰⁵²⁷⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

86D-86D

(Chan, 1997.7.31)

86D-86E

(determined by in situ hybridisation)

(Chan et al., 1998)

86D8-86D10

(determined by in situ hybridisation)

(O'Kane, 1997.9.17)

Experimentally Determined Recombination Data

Location

3-50

(FlyBase, 2016)

3-47.7

(Comeron et al., 2012)

(Chan, 1997.7.31)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

79221

w¹¹¹⁸; P{RpL3-FLAG}2

77132

w¹¹¹⁸; P{UAS-RpL3.FLAG}3

79223

w¹¹¹⁸; P{UAS-RpL3.FLAG}2

30199

w¹¹¹⁸; P{EP}RpL3^G13893 snoRNA:CD23^G13893/TM6C, Sb¹

14269

y¹; P{SUPor-P}RpL3^KG05440 ry⁵⁰⁶/TM3, Sb¹ Ser¹

85575

w¹¹¹⁸; PBac{RB}asRNA:CR31144^e03180 RpL3^e03180/TM6B, Tb¹

FlyORF

F001131

M{UAS-RpL3.ORF.3xHA}ZH-86Fb

VDRC

v109820

P{KK109091}VIE-260B

v46265

w¹¹¹⁸ P{GD16490}v46265

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1201)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020910

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020910

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Nonsense-mediated mRNA decay (NMD) down-regulates a distinct splice isoform(s) of this gene.

(Hansen et al., 2009)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Not a Minute gene.

(Marygold et al., 2007)

Molecularly-defined mutations in RpL3 do not result in Minute phenotypes.

(Marygold et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

snoRNA:50 is encoded in an intron of RpL3.

(Yuan et al., 2003)

RpL3 has been cloned and sequenced.

(Chan et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: RpL3 anon- EST:GressD4

(Ashburner, 2004.6.30)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG4863

(Kolkhof et al., 2017, Fukuoh et al., 2014, Schertel et al., 2013, Coolon et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Müller et al., 2010, Keleman et al., 2009.8.5, Goshima et al., 2007, Marygold et al., 2007, Huang et al., 2005, Accardo et al., 2004, Ashburner, 2004.6.30)

(Anger et al., 2013, Carpenter et al., 2009)

Rpl3

(Weake et al., 2011)

anon-EST:GressD4

chr3R:7047878..7048035

(Will et al., 2013)

rpL3

(Yuan et al., 2003)

Name Synonyms

Ribosomal protein L3

(Cammarato et al., 2011)

ribosomal protein L3

(Accardo et al., 2004)

Secondary FlyBase IDs

FBgn0025328

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41347

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O16797

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O16797

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O16797

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020910

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41347/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020910

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020910

FlyMine - An integrated database for Drosophila genomics

FBgn0020910

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4863

MARRVEL_MODEL - MARRVEL (model organism gene)

41347

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

66481

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020910

DroID - A comprehensive database of gene and protein interactions.

FBgn0020910

DRSC - Results frm RNAi screens

FBgn0020910

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020910

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020910

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4863

Flygut - An atlas of the Drosophila adult midgut

FBgn0020910

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0020910

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005395

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

41347

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (104)

The following external sites may use different assemblies or annotations than FlyBase.

-1.81

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