FlyBase Gene Report: Dmel\CG9577

General Information

Symbol

Dmel\CG9577

Species

D. melanogaster

Name

Annotation Symbol

CG9577

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031092

Gene Model Status

Current

Stock Availability

4 publicly available

Enzyme Name (EC)

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (5.3.3.21)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Ech1

Key Links

Genomic Location

Cytogenetic map

19C1-19C1

Sequence location

X:20,166,673..20,168,080 [+]

Recombination map

1-64

RefSeq locus

NC_004354 REGION:20166673..20168080

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity

inferred from biological aspect of ancestor with PANTHER:PTN000234584

(GO Reference Genome Project, 2011-)

enables isomerase activity

inferred from electronic annotation with InterPro:IPR045002

(InterPro Project Members, 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in fatty acid beta-oxidation

non-traceable author statement

(Faust et al., 2012)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000234585

(GO Reference Genome Project, 2011-)

located_in mitochondrion

inferred from sequence or structural similarity with UniProtKB:Q62651

(Gene Ontology Curators, 2002-)

located_in peroxisome

inferred from sequence model

(Faust et al., 2012)

Gene Group (FlyBase)

INTRAMOLECULAR OXIDOREDUCTASES TRANSPOSING C-C DOUBLE BONDS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity

a (3E,5Z)-dienoyl-CoA = a (2E,4E)-(5,6-saturated)-dienoyl-CoA (5.3.3.21)

RHEA 45240:

Summaries

Gene Group (FlyBase)

INTRAMOLECULAR OXIDOREDUCTASES TRANSPOSING C-C DOUBLE BONDS -: Intramolecular oxidoreductases transposing C-C double bonds catalyze the oxidation of one part of a molecule and the concurrent reduction of another part of the same molecule, and one or more carbon-carbon double bonds in the molecule are rearranged. (Adapted from FBrf0238665.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG9577 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W5W8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG9577-RA

FBtr0070007

NM_134531

1131

312

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG9577-PA

FBpp0070006

33.8

312

7.53

Q9W5W8

NP_608375

AAF45374

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W5W8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG9577 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG9577_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG9577 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG9577

View exonic expression by tissue for Dmel\CG9577

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031092

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33016/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031092

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031092

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031092

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9577

Flygut - An atlas of the Drosophila adult midgut

FBgn0031092

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG9577

Stages(s) 1-3
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG9577

Transgenic constructs containing regulatory region of CG9577

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG9577^GD14253

viable, with Scer\GAL4^Mef2.PR

CG9577^GD14253

viable, with Scer\GAL4^pnr-MD237

CG9577^GD14253

viable, with Scer\GAL4^tin.CΔ4

CG9577^GD14253

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\ECH1

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Ech1

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Ech1

13 of 14

Yes

Mmus\Echdc1

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\ech1

11 of 13

Yes

Xtro\echdc1

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ucn3

1 of 13

Yes

Danio rerio (Zebrafish) (14)

Drer\ech1

14 of 14

Yes

Drer\echdc1

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\F58A6.1

14 of 14

Yes

Cele\Y25C1A.13

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP002661

12 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\DCI1

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\DCI1

1 of 13

Yes

Scer\ECI1

1 of 13

Yes

Scer\EHD3

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC2D10.09

1 of 12

Yes

Escherichia coli (enterobacterium) (7)

Ecol\caiD

1 of 11

Yes

Ecol\fadB

1 of 11

Yes

Ecol\fadJ

1 of 11

Yes

Ecol\menB

1 of 11

Yes

Ecol\paaF

1 of 11

Yes

Ecol\paaG

1 of 11

Yes

Ecol\scpB

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG9577. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Dci

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ECH1; enoyl-CoA hydratase 1

13 of 14

600696

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG9577 - Rab6

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

CG9577 - mPges2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59309

DroID - A comprehensive database of gene and protein interactions.

FBgn0031092

MIST (protein-protein) - An integrated Molecular Interaction Database

33016

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-64

Cytogenetic map

19C1-19C1

Sequence location

X:20,166,673..20,168,080 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19C1-19C1

Limits computationally determined from genome sequence between P{EP}amn^EP1639&P{EP}amn^EP367 and P{EP}CG1702^EP1525

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-64

(FlyBase, 2016)

1-63.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

63247

w^* P{EP}CG9577^G1391B

NIG-Fly

HMJ21760

y¹ v¹; P{TRiP.HMJ21760}attP40/CyO

VDRC

v110267

P{KK108239}VIE-260B

v24063

w¹¹¹⁸; P{GD14253}v24063

Genomic Clones (7)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (24)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE22677

Other clones

DPiM_RE22677

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031092

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031092

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Ech1 CG9577

(Baron et al., 2016)

Source for identity of: CG9577 Ech1

(FlyBase, 2009-)

Nomenclature comments

Reverted 'Ech1' (Enoyl-CoA hydratase 1) back to its CG designation because the gene was named based on the human ortholog in FBrf0232128, but that human protein (UniProt Q13011) is described as an isomerase rather than a hydratase.

(FlyBase, 2009-)

Etymology

Named 'Ech1' after the human ortholog.

(Baron et al., 2016)

Synonyms and Secondary IDs (3)

Reported As

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33016

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W5W8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9W5W8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W5W8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031092

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33016/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031092

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031092

FlyMine - An integrated database for Drosophila genomics

FBgn0031092

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9577

MARRVEL_MODEL - MARRVEL (model organism gene)

33016

Linkouts

BioGRID - A database of protein and genetic interactions.

59309

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031092

DroID - A comprehensive database of gene and protein interactions.

FBgn0031092

DRSC - Results frm RNAi screens

FBgn0031092

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG9577_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031092

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031092

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9577

Flygut - An atlas of the Drosophila adult midgut

FBgn0031092

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0031092

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

MIST (protein-protein) - An integrated Molecular Interaction Database

33016

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

-0.77

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