FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Scot
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General Information
Symbol
Dmel\Scot
Species
D. melanogaster
Name
Succinyl-CoA:3-ketoacid CoA transferase
Annotation Symbol
CG1140
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035298
Gene Model Status
Current
Stock Availability
8 publicly available
Enzyme Name (EC)
3-oxoacid CoA-transferase (2.8.3.5)
Gene Summary
Key enzyme for ketone body catabolism (PubMed:24100554). Transfers the CoA moiety from succinate to acetoacetate (PubMed:24100554). Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity). (UniProt, Q9W058)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

3-oxoacid CoA-transferase

Key Links
Genomic Location
Cytogenetic map
62B11-62B12
Sequence location
Recombination map
3-1.0
RefSeq locus
NT_037436 REGION:1963143..1966805
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables succinyl-CoA:3-oxo-acid CoA-transferase activity
inferred from direct assay
(Zhang et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables CoA-transferase activity
(InterPro Project Members, 2004-)
inferred from electronic annotation with InterPro:IPR004163, InterPro:IPR004165
(InterPro Project Members, 2004-)
(InterPro Project Members, 2004-)
enables succinyl-CoA:3-oxo-acid CoA-transferase activity
inferred from biological aspect of ancestor with PANTHER:PTN000344750
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:Q29551
(Kasravi et al., 1999)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in ketone body catabolic process
inferred from electronic annotation with InterPro:IPR014388
(InterPro Project Members, 2004-)
involved_in ketone body metabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000344750
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P55809
(Gene Ontology Curators, 2002-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN001009021
(GO Reference Genome Project, 2011-)
located_in mitochondrion
inferred from sequence or structural similarity with UniProtKB:P55809
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the 3-oxoacid CoA-transferase family. (Q9W058)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
succinyl-CoA:3-oxo-acid CoA-transferase activity
a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate (2.8.3.5)
RHEA 24564:
Summaries
Gene Group (FlyBase)
COA-TRANSFERASES -
CoA-transferases catalyse the transfer of a coenzyme A (CoA) group from one compound (donor) to another (acceptor).
Protein Function (UniProtKB)
Key enzyme for ketone body catabolism (PubMed:24100554). Transfers the CoA moiety from succinate to acetoacetate (PubMed:24100554). Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).
(UniProt, Q9W058)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Scot for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W058)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Scot-RA
FBtr0072844
NM_139426
1768
516
Scot-RB
FBtr0072843
NM_167928
1966
502
Scot-RC
FBtr0333431
NM_001274372
2006
450
Additional Transcript Data and Comments
Reported size (kB)

1.8 (longest cDNA)

(Kasravi et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Scot-PA
FBpp0072723
54.9
516
8.61
Q9W058
NP_647683
AAF47600
Scot-PB
FBpp0072722
53.4
502
6.93
Q8IRG3
NP_728699
AAN11508
Scot-PC
FBpp0305622
48.0
450
8.15
M9PGX7
NP_001261301
AGB93996
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9W058)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Scot using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.23

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Scot in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Scot
Transgenic constructs containing regulatory region of Scot
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\OXCT1
14 of 14
Yes
Yes
Hsap\OXCT2
13 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Oxct1
14 of 14
Yes
Yes
Rnor\Oxct2b
11 of 14
No
Yes
Rnor\Oxct2a
1 of 14
No
Yes
Mus musculus (laboratory mouse) (3)
Mmus\Oxct1
14 of 14
Yes
Yes
Mmus\Oxct2b
13 of 14
No
Yes
Mmus\Oxct2a
12 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\oxct1
7 of 13
Yes
Yes
Danio rerio (Zebrafish) (3)
Drer\oxct1a
14 of 14
Yes
Yes
Drer\oxct1b
14 of 14
Yes
Yes
Drer\LOC797311
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\C05C10.3
14 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP006096
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (3)
Ecol\atoA
7 of 11
Yes
Yes
Ecol\ydiF
4 of 11
No
Yes
Ecol\atoD
3 of 11
No
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Scot. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    OXCT1; 3-oxoacid CoA-transferase 1
    14 of 14
    601424
      OXCT2; 3-oxoacid CoA-transferase 2
      13 of 14
      610289
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          Interaction Browsers
          Summary of Genetic Interactions
          Interaction Browsers
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          External Data
          Subunit Structure (UniProtKB)
          Homodimer.
          (UniProt, Q9W058 )
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          FlyBase
          External Links
          KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
          External Data
          Linkouts
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Class of Gene
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3L
          Recombination map
          3-1.0
          Cytogenetic map
          62B11-62B12
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          62B11-62B12
          Limits computationally determined from genome sequence between P{PZ}dlt04276 and P{PZ}slsrL182
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          3-1.0

          (FlyBase, 2016)

          3-3.4

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (8)
          Genomic Clones (15)
           

          Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

          cDNA Clones (45)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              Identification: transcription unit identified during molecular analysis of the mu2 genomic region.

              (Kasravi et al., 1999)
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: CG1140 3-oxoacid-CoA-transferase

              (FlyBase, 1996-)

              Source for merge of: CG1140 BcDNA:RH54340

              (FlyBase, 1996-)
              Additional comments

              Source for merge of CG1140 BcDNA:RH54340 was a shared cDNA ( date:030728 ).

              (FlyBase, 1996-)
              Nomenclature History
              Source for database identify of

              Source for identity of: Scot CG1140

              (Zhang et al., 2013)
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (12)
              Reported As
              Symbol Synonym
              BcDNA:RH54340
              CG1140
              (Aradska et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Teleman et al., 2008, Rehmsmeier et al., 2004)
              CT1116
              (Ceriani et al., 2002)
              DmSCOT
              (Zhang et al., 2013)
              SCOT
              (Haas et al., 2025, François et al., 2023, Wang et al., 2021, Transgenic RNAi Project members, 2017-)
              Scot
              (Krejčová et al., 2025, Beghelli et al., 2024)
              cDNA B
              (Kasravi et al., 1999)
              Name Synonyms
              3-oxoacid CoA-transferase
              (Teleman et al., 2008, Kasravi et al., 1999)
              3-oxoacid-CoA transferase
              (Celotto et al., 2011)
              3-oxoacid-CoA-transferase
              Succinyl- CoA:3-ketoacid CoA transferase
              (Zhang et al., 2013)
              citric acid synthetase
              (Kasravi et al., 1999)
              Secondary FlyBase IDs
              • FBgn0027933
              • FBgn0064797
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 62 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              PDB - An information portal to biological macromolecular structures
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
              References (51)