FlyBase Gene Report: Dmel\CG31145

General Information

Symbol

Dmel\CG31145

Species

D. melanogaster

Name

Annotation Symbol

CG31145

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051145

Gene Model Status

Current

Stock Availability

18 publicly available

Enzyme Name (EC)

Gene Summary

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs. (UniProt, A4VCL2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

95A4-95A7

Sequence location

3R:23,605,751..23,669,659 [-]

Recombination map

3-80

RefSeq locus

NT_033777 REGION:23605751..23669659

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables protein kinase activity

inferred from sequence or structural similarity with FLYBASE:fj; FB:FBgn0000658

(Ishikawa et al., 2012)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000278746

(GO Reference Genome Project, 2011-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Ishikawa et al., 2012)

located_in Golgi apparatus

inferred from direct assay

(Ishikawa et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000278680

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ATYPICAL PROTEIN KINASES

Protein Family (UniProt)

Belongs to the FAM20 family. (A4VCL2)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Group (FlyBase)

ATYPICAL PROTEIN KINASES -: Atypical kinases are protein kinases or putative protein kinases that do not share clear sequence similarity with conventional eukaryotic protein kinases, but they or their orthologs have been shown experimentally to have protein kinase activity. Atypical kinases are defined in FBrf0201870.

Protein Function (UniProtKB)

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs.

(UniProt, A4VCL2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG31145 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A4VCL2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Stop-codon suppression (UAG) postulated; FBrf0234051.

Gene model reviewed during 6.25

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG31145-RA

FBtr0084479

NM_170079

2586

528

CG31145-RB

FBtr0084480

NM_176547

2595

528

CG31145-RC

FBtr0290056

NM_001144629

4524

980

CG31145-RD

FBtr0290057

NM_001144630

3519

915

CG31145-RF

FBtr0336459

NM_001144631

3714

528

CG31145-RG

FBtr0474143

NM_001369999

4524

982

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG31145-PA

FBpp0083870

59.4

528

8.23

FBpp0083871

59.4

528

8.23

FBpp0288495

109.7

980

8.39

FBpp0288496

102.6

915

8.80

NP_001138102

ACL83558

CG31145-PF

FBpp0307565

59.4

528

8.23

FBpp0423142

109.9

982

8.51

QCD25206

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

528 aa isoforms: CG31145-PA, CG31145-PB, CG31145-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG31145 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG31145_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.09

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

midline primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 13

midline glial cell

(Wheeler et al., 2006)

embryonic posterior midline glial cell

(Wheeler et al., 2006)

embryonic stage 16

embryonic/larval salivary gland

(Ishikawa et al., 2012)

embryonic/larval dorsal vessel

(Ishikawa et al., 2012)

midline glial cell

(Ishikawa et al., 2012)

embryonic/larval midgut

(Ishikawa et al., 2012)

Additional Descriptive Data

Expression pattern inferred from unspecified enhancer trap line.

(Kearney et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Ishikawa et al., 2012)

located_in Golgi apparatus

inferred from direct assay

(Ishikawa et al., 2012)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.0}CG31145^{MI00781-TG4.0}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018)

Reporter: P{ST.lexA::HG}CG31145^JPC-7

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG31145 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG31145

View exonic expression by tissue for Dmel\CG31145

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051145

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42784/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051145

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051145

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051145

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31145

Flygut - An atlas of the Drosophila adult midgut

FBgn0051145

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG31145

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG31145

Transgenic constructs containing regulatory region of CG31145

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage

CG31145^BG00449

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG31145^GD8687

viable, with Scer\GAL4^Mef2.PR

CG31145^GD8687

viable, with Scer\GAL4^pnr-MD237

CG31145^GD8687

viable, with Scer\GAL4^tin.CΔ4

CG31145^GD8687

Other Phenotypes

Allele

abnormal mating | male, with Scer\GAL4^Abd-B.D1

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\FAM20C

11 of 14

Yes

Hsap\FAM20A

8 of 14

Yes

Hsap\FAM20B

3 of 14

Hsap\FJX1

1 of 14

Hsap\SAMD7

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Fam20c

11 of 14

Yes

Rnor\Fam20a

8 of 14

Yes

Rnor\Fam20b

3 of 14

Rnor\RGD1559578

1 of 14

Yes

Rnor\Samd7

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Fam20c

11 of 14

Yes

Mmus\Fam20a

8 of 14

Yes

Mmus\Fam20b

2 of 14

Mmus\1700012B07Rik

1 of 14

Yes

Mmus\Samd7

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\LOC100494742

8 of 13

Yes

Xtro\fam20c

6 of 13

Yes

Xtro\fam20a

4 of 13

Yes

Xtro\fam20a.2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (7)

Drer\fam20ca

11 of 14

Yes

Drer\fam20cb

11 of 14

Yes

Drer\fam20cl

10 of 14

Yes

Drer\fam20a

8 of 14

Yes

Drer\fam20b

3 of 14

Drer\LOC100536075

3 of 14

Yes

Drer\samd7

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\famk-1

12 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP002913

11 of 12

Yes

Agam\AgaP_AGAP001051

3 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG31145. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG3631

5 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

FAM20A; FAM20A golgi associated secretory pathway pseudokinase

model of amelogenesis imperfecta type 1G

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

FAM20C; FAM20C golgi associated secretory pathway kinase

11 of 14

611061

RAINE SYNDROME; RNS

FAM20A; FAM20A golgi associated secretory pathway pseudokinase

8 of 14

611062

AMELOGENESIS IMPERFECTA, TYPE IG; AI1G

amelogenesis imperfecta type 1G

FAM20B; FAM20B glycosaminoglycan xylosylkinase

3 of 14

611063

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67724

DroID - A comprehensive database of gene and protein interactions.

FBgn0051145

MIST (protein-protein) - An integrated Molecular Interaction Database

42784

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2016)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-80

Cytogenetic map

95A4-95A7

Sequence location

3R:23,605,751..23,669,659 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95A4-95A7

Limits computationally determined from genome sequence between P{PZ}cnc⁰³⁹²¹ and P{PZ}l(3)06906⁰⁶⁹⁰⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-80

(FlyBase, 2016)

3-81.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Genomic Clones (50)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (51)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE73615

Other clones

IP18281

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051145

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051145

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG10187 CG13820 CG13821

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG10187, CG13820 and CG13821 merged as CG31145 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

FBgn0039095
FBgn0039096
FBgn0039097

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42784

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A4VCL2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A4VCL2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A4D6K5D6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A4VCL2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051145

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42784/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051145

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051145

FlyMine - An integrated database for Drosophila genomics

FBgn0051145

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31145

MARRVEL_MODEL - MARRVEL (model organism gene)

42784

Linkouts

BioGRID - A database of protein and genetic interactions.

67724

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051145

DroID - A comprehensive database of gene and protein interactions.

FBgn0051145

DRSC - Results frm RNAi screens

FBgn0051145

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG31145_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051145

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051145

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31145

Flygut - An atlas of the Drosophila adult midgut

FBgn0051145

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0051145

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034167

MIST (protein-protein) - An integrated Molecular Interaction Database

42784

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (59)

The following external sites may use different assemblies or annotations than FlyBase.

-1.09

Report Sections