FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Gene: Dmel\Ptip
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General Information
Symbol
Dmel\Ptip
Species
D. melanogaster
Name
PAX transcription activation domain interacting protein
Annotation Symbol
CG32133
Feature Type
protein_coding_gene
FlyBase ID
FBgn0052133
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Summary
PAX transcription activation domain interacting protein (Ptip) encodes a component of a Histone H3K4 methyltransferase complex that contributes to Trithorax-mediated epigenetic imprinting during development. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
70C12-70C14
Sequence location
Recombination map
3-41
RefSeq locus
NT_037436 REGION:14020315..14029121
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in anterior/posterior pattern specification, imaginal disc
inferred from mutant phenotype
(Fang et al., 2009)
involved_in chromatin remodeling
inferred from mutant phenotype
(Zhu et al., 2024)
involved_in embryonic pattern specification
inferred from mutant phenotype
(Fang et al., 2009)
involved_in heart development
inferred from mutant phenotype
(Zhu et al., 2024)
involved_in positive regulation of antibacterial peptide biosynthetic process
inferred from mutant phenotype
(Valanne et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of MLL3/4 complex
inferred from direct assay
(Mohan et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MLL3/4 complex
inferred from biological aspect of ancestor with PANTHER:PTN002754812
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000574320
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
PAX transcription activation domain interacting protein (Ptip) encodes a component of a Histone H3K4 methyltransferase complex that contributes to Trithorax-mediated epigenetic imprinting during development. [Date last reviewed: 2018-11-15]
Gene Group (FlyBase)
TRR COMPLEX -
The Trithorax-related (Trr) complex is a COMPASS-like complex that mediates the methylation of histone H3K4 and demethylation of histone H3K27. (Adapted from FBrf0216343 and FBrf0218498).
Summary (Interactive Fly)

BRCT (BRCA1 carboxy terminal) domain protein that can bind to phosphorylated serine residues - chromatin-binding protein implicated in epigenetic control of development and differentiation - potential trithorax gene cofactor - potential interactor with transcription factors that also serves as a modifier of histones

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Ptip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VUB7)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ptip-RA
FBtr0075784
NM_168571
8068
2294
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Ptip-PA
FBpp0075526
262.5
2294
9.96
Q9VUB7
NP_729947
AAF49771
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ptip using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.73

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Fang et al., 2009)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Fang et al., 2009)
Additional Descriptive Data

Ptip is expressed in all developmental stages on northern blots and is more abundant in females than males. It is expressed ubiquitously in embryos as shown by in situ hybridization.

(Fang et al., 2009)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of MLL3/4 complex
inferred from direct assay
(Mohan et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ptip in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ptip
Transgenic constructs containing regulatory region of Ptip
Aberrations (Deficiencies and Duplications) ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\PAXIP1
9 of 14
Yes
Yes
Hsap\MDC1
2 of 14
No
Yes
Hsap\TOPBP1
2 of 14
No
No
Hsap\ECT2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Paxip1
9 of 14
Yes
Yes
Rnor\Topbp1
2 of 14
No
No
Rnor\Ect2
1 of 14
No
No
Mus musculus (laboratory mouse) (3)
Mmus\Paxip1
9 of 14
Yes
Yes
 
1  
Mmus\Topbp1
3 of 14
No
No
Mmus\Mdc1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\paxip1
5 of 13
Yes
Yes
Xtro\XB5726496
4 of 13
No
Yes
Xtro\ect2
1 of 13
No
No
Xtro\topbp1
1 of 13
No
No
Danio rerio (Zebrafish) (4)
Drer\paxip1
8 of 14
Yes
Yes
Drer\topbp1
2 of 14
No
No
Drer\ect2
1 of 14
No
No
Drer\mdc1
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\pis-1
8 of 14
Yes
Yes
Cele\mus-101
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP001710
9 of 12
Yes
Yes
Agam\AgaP_AGAP002760
1 of 12
No
No
Agam\AgaP_AGAP005374
1 of 12
No
No
Agam\AgaP_AGAP005796
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\AT3G21480
4 of 13
Yes
Yes
Atha\AT4G03130
4 of 13
Yes
Yes
Atha\AT1G67180
2 of 13
No
Yes
Atha\AT2G41450
2 of 13
No
No
Atha\AT4G02110
1 of 13
No
No
Atha\MEI1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\RTT107
2 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\brc1
5 of 12
Yes
Yes
Spom\mdb1
3 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Ptip. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
mu2
1 of 13
mus101
1 of 13
pbl
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Ptip3804
ameliorates  cancer
modeled by Ras85DV12.cUa.UAS
(Dong et al., 2021)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
PAXIP1; PAX interacting protein 1
9 of 14
608254
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Dmel gene
      Ortholog showing functional complementation
      Supporting References
      Ptip
      Mmus\Paxip1
      (Fang et al., 2009)
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Ptip - Bap60
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - CG17002
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - CG3008
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - CG34159
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - Cdk8
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - Cpsf6
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED10
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED11
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED14
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED15
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED16
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED17
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED18
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED20
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED23
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED27
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED30
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED4
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED6
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED7
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - MED8
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - Pa1
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Mohan et al., 2011)
      Ptip - Ssdp
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - Usp10
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - Utx
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Mohan et al., 2011)
      Ptip - ash2
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Mohan et al., 2011)
      Ptip - boca
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - brm
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - dsh
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - ebi
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - enc
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - ix
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - mor
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - osa
      experimental knowledge based
      (Guruharsha et al., 2011)
      Ptip - skd
      experimental knowledge based
      (Guruharsha et al., 2011)
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-41
      Cytogenetic map
      70C12-70C14
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      70C12-70C14
      Limits computationally determined from genome sequence between P{EP}EP3561EP3561 and P{PZ}l(3)70Da02402&P{PZ}btl00208
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-41

      (FlyBase, 2016)

      3-35.6

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (11)
      Genomic Clones (14)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (7)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: CG6532 CG8797

              (FlyBase Genome Annotators, 2002-2003)
              Additional comments

              Annotations CG6532 and CG8797 merged as CG32133 in release 3 of the genome annotation.

              (FlyBase Genome Annotators, 2002-2003)
              Nomenclature History
              Source for database identify of

              Source for identity of: ptip CG32133

              (Fang et al., 2009)

              Source for identity of: Ptip ptip

              (FlyBase, 2009-)
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (8)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 31 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Flygut - An atlas of the Drosophila adult midgut
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              References (59)