FB2026_02 , released June 18, 2026
Gene: Dmel\inaE
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General Information
Symbol
Dmel\inaE
Species
D. melanogaster
Name
inactivation no afterpotential E
Annotation Symbol
CG33174
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261244
Gene Model Status
Current
Stock Availability
14 publicly available
Enzyme Name (EC)
lipoprotein lipase (3.1.1.34)
Gene Summary
inactivation no afterpotential E (inaE) encodes a diacylglycerol lipase involved in phototransduction and response to oxidative stress. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

diacylglycerol lipase, DAGL

Key Links
Genomic Location
Cytogenetic map
12C4-12C5
Sequence location
Recombination map
1-46
RefSeq locus
NC_004354 REGION:13783714..13811493
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables lipoprotein lipase activity
inferred from direct assay
(Leung et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables lipoprotein lipase activity
inferred from biological aspect of ancestor with PANTHER:PTN008461757
(GO Reference Genome Project, 2011-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in phototransduction
inferred from mutant phenotype
(Leung et al., 2008)
involved_in response to oxidative stress
inferred from mutant phenotype
(Lin et al., 2014)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in arachidonate metabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000486522
(GO Reference Genome Project, 2011-)
involved_in diacylglycerol catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000486522
(GO Reference Genome Project, 2011-)
involved_in lipid metabolic process
inferred from electronic annotation with InterPro:IPR002921
(InterPro Project Members, 2004-)
involved_in neurogenesis
inferred from biological aspect of ancestor with PANTHER:PTN008461757
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Leung et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in dendrite membrane
inferred from biological aspect of ancestor with PANTHER:PTN008461757
(GO Reference Genome Project, 2011-)
is_active_in postsynaptic membrane
inferred from biological aspect of ancestor with PANTHER:PTN008461757
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
lipoprotein lipase activity
a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (3.1.1.34)
Summaries
Gene Snapshot
inactivation no afterpotential E (inaE) encodes a diacylglycerol lipase involved in phototransduction and response to oxidative stress. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
UNCLASSIFIED TRIGLYCERIDE LIPASES -
This group comprises triglyceride lipases that do not classify under other groups in FlyBase.
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
inaE (J.C. Hall)
Same basic eye-physiology phenotype as in inaA, inaB, and inaC; one allele (at least, i.e. inaE1) causes age-dependent photoreceptor degeneration.
Summary (Interactive Fly)

diacylglycerol lipase involved in visual signal transduction and lipid metabolism

Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
7

Please see the JBrowse view of Dmel\inaE for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry B7Z150)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Gene model reviewed during 6.43

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
inaE-RA
FBtr0073826
NM_176728
4004
644
inaE-RD
FBtr0073825
NM_176727
5910
737
inaE-RE
FBtr0290302
NM_001144723
5086
1318
inaE-RF
FBtr0290303
NM_001144724
5698
815
inaE-RG
FBtr0340290
NM_001298301
5062
1310
inaE-RH
FBtr0340291
NM_001298302
5973
729
inaE-RI
FBtr0481627
NM_001414079
3749
708
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
inaE-PA
FBpp0073657
73.4
644
8.28
Q8MR12
NP_788901
AAO41653
inaE-PD
FBpp0073656
83.9
737
8.43
Q86B55
NP_788900
AAO41652
inaE-PE
FBpp0288741
144.9
1318
7.64
B7Z150
NP_001138195
ACL82927
inaE-PF
FBpp0288742
92.4
815
7.89
B7Z151
NP_001138196
ACL82928
inaE-PG
FBpp0309251
143.9
1310
7.57
X2JBS2
NP_001285230
AHN59700
inaE-PH
FBpp0309252
82.9
729
8.36
X2JDP4
NP_001285231
AHN59701
inaE-PI
FBpp0428409
80.6
708
7.84
UYK33059
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\inaE using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.04

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
larval abdominal intersegmental nerve neuron
(Tortoriello et al., 2021)
larval stage
larval brain
(Tortoriello et al., 2021)
eye disc
(Tortoriello et al., 2021)
rhabdomere
(Tortoriello et al., 2021)
adult stage
calyx of adult mushroom body
(Tortoriello et al., 2021)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

inaE protein localizes to intersegmental neuroblast/neuron clusters along the ventral nerve cord. The expression is dynamic during development. Postembryonically, the protein is found in the larval brain and eye discs, and particularly localizes to photoreceptor rhabdomeres and the dendritic field of the calyx region of the mushroom bodies.

(Tortoriello et al., 2021)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Leung et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\inaE in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of inaE
Transgenic constructs containing regulatory region of inaE
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(1)AR10
(Leung et al., 2008)
Df(1)benCO2
(Leung et al., 2008)
Inferred from location ( 9 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\DAGLA
11 of 14
Yes
Yes
Hsap\DAGLB
5 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Dagla
11 of 14
Yes
Yes
Rnor\Daglb
4 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Dagla
11 of 14
Yes
Yes
Mmus\Daglb
4 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\dagla
7 of 13
Yes
Yes
Xtro\daglb
2 of 13
No
No
Xtro\LOC100486956
1 of 13
No
Yes
Danio rerio (Zebrafish) (2)
Drer\dagla
10 of 14
Yes
Yes
Drer\daglb
5 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\dagl-2
7 of 14
Yes
Yes
Cele\K08B12.1
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP000945
12 of 12
Yes
Yes
Agam\AgaP_AGAP012171
3 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (28)
Atha\AT1G05790
5 of 13
Yes
Yes
Atha\AT2G42450
5 of 13
Yes
Yes
Atha\AT3G14075
5 of 13
Yes
Yes
Atha\AT4G16070
5 of 13
Yes
Yes
Atha\AT3G49050
3 of 13
No
Yes
Atha\AT4G00500
3 of 13
No
Yes
Atha\AT5G37710
3 of 13
No
Yes
Atha\AG
1 of 13
No
No
Atha\AGL6
1 of 13
No
No
Atha\AGL8
1 of 13
No
No
Atha\AGL13
1 of 13
No
No
Atha\AGL30
1 of 13
No
No
Atha\AGL65
1 of 13
No
No
Atha\AGL66
1 of 13
No
No
Atha\AGL67
1 of 13
No
No
Atha\AGL79
1 of 13
No
No
Atha\AGL94
1 of 13
No
No
Atha\AGL104
1 of 13
No
No
Atha\AP1
1 of 13
No
No
Atha\CAL
1 of 13
No
No
Atha\CAX9
1 of 13
No
No
Atha\SEP1
1 of 13
No
No
Atha\SEP2
1 of 13
No
No
Atha\SEP3
1 of 13
No
No
Atha\SEP4
1 of 13
No
No
Atha\SHP1
1 of 13
No
No
Atha\SHP2
1 of 13
No
No
Atha\STK
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:inaE. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DAGLA; diacylglycerol lipase alpha
    11 of 14
    614015
      DAGLB; diacylglycerol lipase beta
      5 of 14
      614016
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          Interaction Browsers
          Summary of Genetic Interactions
          Interaction Browsers
          View in FlyBase Interactions Browser   View in MIST tool (external link)
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          pnr
          suppressible
          inaE
          (Pena-Rangel et al., 2002)
          trp
          enhanceable
          inaE
          (Leung et al., 2008)
          External Data
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          FlyBase
          External Links
          FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
          External Data
          Linkouts
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Class of Gene
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          X
          Recombination map
          1-46
          Cytogenetic map
          12C4-12C5
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          12C4-12C5
          Limits computationally determined from genome sequence between P{EP}CG33174EP1101 and P{EP}EP1595EP1595
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          1-46

          (FlyBase, 2016)

          1-44.0

          (Comeron et al., 2012)

          1-

          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (14)
          Genomic Clones (20)
          cDNA Clones (79)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Tortoriello et al., 2021, Leung et al., 2008)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: inaE CG33174

                (Leung et al., 2008)

                Source for merge of: CG11102 CG11104 CG15761 CG11096

                (FlyBase Genome Annotators, 2002-2003)
                Additional comments

                Annotations CG11102, CG11104, CG15761 and CG11096 merged as CG33174 in release 3 of the genome annotation.

                (FlyBase Genome Annotators, 2002-2003)
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (14)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 74 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (62)