mod(mdg4), Cp190 Physical Interaction report

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)2.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Pai et al., 2004)

FBrf0180528-3.Y2H

Reference

Pai et al., 2004

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

fused to GAL4 DNA-binding domain

mod(mdg4)

Mod(mdg4)2.2

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pai et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 2004)

FBrf0180528-5.Y2H

Reference

Pai et al., 2004

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod(mdg4)2.2

bait

fused to GAL4 DNA-binding domain

Cp190

CP190

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pai et al., 2004)

Cp190 binds to mod(mdg4), but not another BTB-domain-containing protein, Trl (GAGA factor).

(Pai et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 2004)

FBrf0194107-4.coIP.WB

Reference

Lei and Corces, 2006

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)2.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The interaction of mod(mdg4) with Cp190 is RNA-independent (not affected by RNaseA treatment).

(Lei and Corces, 2006)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lei and Corces, 2006)

Positive control.

(Lei and Corces, 2006)

FBrf0201197-3.coIP.WB

Reference

Gerasimova et al., 2007

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod2.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gerasimova et al., 2007)

FBrf0212850-1.coIP.WB

Reference

Oliver et al., 2010

Description

physical association

Assay

Collection

Source/Stage

organism / pupal stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)67.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was pupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oliver et al., 2010)

FBrf0214686-5.CH.WB

Reference

Moshkovich et al., 2011

Description

physical association

Assay

Collection

Source/Stage

embryo / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

bait

mod(mdg4)

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Moshkovich et al., 2011)

FBrf0218398-2.coIP.WB

Reference

Golovnin et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)-67.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2012)

FBrf0218398-4.coIP.WB

Reference

Golovnin et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod(mdg4)-67.2

bait

Cp190

CP190

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2012)

FBrf0221054-1.coIP.WB

Reference

Lim et al., 2013

Description

physical association

Assay

anti tag coimmunoprecipitation

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)2.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Positive control.

(Lim et al., 2013)

Exosome RNase activity copurifies with Cp190 immunoprecipitated complexes.

(Lim et al., 2013)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lim et al., 2013)

FBrf0224437-4.coIP.MS

Reference

Cuartero et al., 2014

Description

physical association

Assay

peptide massfingerprinting

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

TAP tag

mod(mdg4)

Mod(mdg4)67.2

prey

7 peptides identified

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Cuartero et al., 2014)

TAP tandem affinity purification of bait and identification of prey by mass spectrometry.

(Cuartero et al., 2014)

FBrf0227255-3.coIP.MS

Reference

Maksimenko et al., 2015

Description

physical association

Assay

anti tag coimmunoprecipitation

peptide massfingerprinting

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

FLAG tag

mod(mdg4)

prey

8 unique peptides

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

mod(mdg4) enriched 12-fold in Cp190 purification compared to negative control.

(Maksimenko et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Maksimenko et al., 2015)

FBrf0234617-2.Y2H

Reference

Golovnin et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod(mdg4)67.2

bait

fused to GAL4 DNA-binding domain

Cp190

CP190

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Golovnin et al., 2016)

FBrf0236960-5.Y2H

Reference

Melnikova et al., 2017

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod-67.2

bait

fused to GAL4 DNA-binding domain

mod(mdg4)

Cp190

CP190

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

mod(mdg4)

BTB domain

sufficient binding region

aa 1-118

coordinates relative to mod(mdg4)-PT

mod(mdg4)

FLYWCH domain

mutation decreasing interaction

aa 454-567

deletion of aa 454-567, coordinates relative to mod(mdg4)-PT

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2017)

FBrf0236960-6.Y2H

Reference

Melnikova et al., 2017

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

fused to GAL4 DNA-binding domain

Cp190

mod(mdg4)

Mod-67.2

prey

fused to GAL4 activation domain

mod(mdg4)

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

mod(mdg4)

BTB domain

sufficient binding region

aa 1-118

coordinates relative to mod(mdg4)-PT

Cp190

interaction domain

sufficient binding region

aa 263-470

Cp190

interaction domain

necessary binding region

aa 309-470

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2017)

FBrf0241934-4.Y2H

Reference

Melnikova et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

fused to GAL4 DNA-binding domain

mod(mdg4)

Mod(mdg4)-67.2

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Melnikova et al., 2019)

FBrf0241934-7.Y2H

Reference

Melnikova et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod(mdg4)-67.2

bait

fused to GAL4 DNA-binding domain

Cp190

CP190

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Melnikova et al., 2019)

FBrf0244288-7.PD.WB

Reference

Melnikova et al., 2019

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

GST tag

Cp190

mod(mdg4)

Mod-67.2

prey

His tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Cp190

M and ZF domains

sufficient binding region

aa 308-595

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Melnikova et al., 2019)

FBrf0249435-15.coIP.MS

Reference

Bag et al., 2021

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)67.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)

FBrf0249435-5.coIP.WB

Reference

Bag et al., 2021

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

mod(mdg4)

Mod(mdg4)67.2

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)

FBrf0249435-7.coIP.WB

Reference

Bag et al., 2021

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

Mod(mdg4)67.2

bait

Cp190

CP190

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)

FBrf0253430-36.PD.MS

Reference

Kaushal et al., 2022

Description

physical association

Assay

pull down

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

bait

GFP tag

mod(mdg4)

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)

FBrf0254231-12.coIP.MS

Reference

Chen et al., 2022

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

bait

mod(mdg4)

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic nuclear extracts of a wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 2022)

FBrf0257791-10.Y2H

Reference

Melnikova et al., 2023

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cp190

CP190

bait

fused to GAL4 DNA-binding domain

mod(mdg4)

Mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4)

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control

(Melnikova et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2023)

FBrf0257791-14.Y2H

Reference

Melnikova et al., 2023

Description

physical association

Assay