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General Information
Symbol
Dmel\P{PZ}gcmrA87
Species
D. melanogaster
Name
FlyBase ID
FBti0009197
Also Known As
rA87, gcm-lacZ, gcm-lacZrA87
Description
Inserted Element
Uses

lacZ reporter

Affected gene(s)
Viability / fertility
viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2L ( 30B12 )
Sequence Location
2L:9,581,826..9,581,826 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:9,581,826..9,581,826 [-]
Orientation
Cytological location (computed by FlyBase)
30B12 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
lacZ enhancer trap
Size (Kb)

14.545

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In the embryo, Ecol\lacZgcm-rA87 drives expression in the peritracheal cells and in two symmetrical clusters in the procephalic mesoderm. The cells in the latter do not colocalise with elav or pros.

Ecol\lacZgcm-rA87 labels the younger glial cells that are generated postembryonically in the larval brain, including the glial cells produced by type II (secondary) neuroblasts. At the early third instar when cells first start expressing Ecol\lacZgcm-rA87 they might also co-express the neuronal markers elav or pros.

In addition to expression in glial precursor cells and lamina precursor cells in the third instar larval optic lobe, reporter expression is observed in medulla neuropil glial cells.

In the larval visual system, Ecol\lacZgcm-rA87 is expressed not only in specific glial subtypes, but also in the lineage that gives rise to lamina neurons. Ecol\lacZgcm-rA87 is expressed in epithelial glia, marginal glia, and some medulla neuropil glia, but not in medulla or lobula glia.

Enhancer trap expression is observed at embryonic stage 8 in the procephalic mesoderm. Later, it is observed in all hemocytes.

Enhancer trap expression first occurs in an anterior ventral patch in the cellular blastoderm embryo. During gastrulation, these cells invaginate at the end of the ventral furrow just anterior to the cephalic furrow in an area that gives rise to the cephalic mesoderm. During stages 11-12, in each hemisegment, sequential and transient expression occurs in two patches of 3-5 ectodermal cells. From each patch, a single large blast cell delaminates and maintains expression. These are tentatively identified as the peripheral glioblast and the longitudinal glioblast. By late stage 11, enhancer trap expression occurs in all of the progeny of these glioblasts as well as in additional glial cells. In the brain lobes, a complex pattern of expression is seen through stage 17. In the PNS, expression occurs in all repo-positive glia along peripheral axon pathways and associated with sensory organs.

By late stage 11, enhancer trap expression occurs in all of the progeny of these glioblasts as well as in additional glial cells. In the brain lobes, a complex pattern of expression is seen through stage 17. In the PNS, expression occurs in all repo-positive glia along peripheral axon pathways and associated with sensory organs.

 
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Position based on author statement that insertion is 109bp upstream of longest cDNA in FBrf0084071.

Synonyms and Secondary IDs (10)
References (42)