FlyBase Recombinant Construct Report: P{GMR58E02-lexA}

General Information

Symbol

P{GMR58E02-lexA}

FlyBase ID

FBtp0088857

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lexA^GMR58E02

Also Known As

R58E02-LexA, 58E02-LexA, 58E02-LexAp65

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Ecol\lexA^GMR58E02

Transgenic product class

Component

Use(s)

Regulatory region(s)

GMR58E02

Encoded product / tool

lexA::p65

binary expression system - driver

Description

A 1235 base pair fragment from the flanking non-coding or intronic region of DAT is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lexA^GMR58E02

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron h1

(Eschbach et al., 2020)

larval mushroom body dopaminergic neuron j1

(Eschbach et al., 2020)

larval mushroom body dopaminergic neuron i1

(Eschbach et al., 2020)

Additional Information

Statement

Reference

Drives expression in dopaminergic PAM neurons and larval dopaminergic pPAM neurons.

(FlyBase Curators, 2017)

EcollexA^GMR58E02 positive dopaminergic PAM neurons are in close proximity with ScerGAL4^GMR66C08 expressing mushroom body medial lobe arborizing neuron 6 as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

(Owald et al., 2015)

Some dopaminergic PAM neurons labelled by EcollexA^GMR58E02 are also co-labelled by ScerGAL4^ple.PF.

(Liu et al., 2012)

Marker for

Reflects expression of

DAT

(Liu et al., 2012)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

52740

w¹¹¹⁸; P{GMR58E02-lexA}attP40

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

58E02

(FlyBase, 1996-)

58E02-LexA

(Yamagata et al., 2016)

58E02-LexAp65

(Eschbach et al., 2020, Aso et al., 2019)

58E02-lexA

(Zolin et al., 2021)

GMR58E02-lexA

(Ahmed et al., 2023)

P{GMR58E02-lexA}

(FlyBase, 1996-)

R58E02-LexA

(Mohammad et al., 2024, Sun et al., 2024, Jelen et al., 2023, Mancini et al., 2023, Shen et al., 2023, Yamazaki et al., 2023, Lei et al., 2022, Pribbenow et al., 2022, Yamagata et al., 2022, French et al., 2021, Vrontou et al., 2021, Dag et al., 2019, Handler et al., 2019, Lyutova et al., 2019, Rohwedder et al., 2016, Cohn et al., 2015, Owald et al., 2015, Yamagata et al., 2015, Liu et al., 2012)

R58E02-LexAp65

(Aso et al., 2019)

R58E02-lexA

(Lozada-Perdomo et al., 2025, Haynes et al., 2015)

R58E2-LexA

(Harris et al., 2015)

Secondary FlyBase IDs

References (30)

Report Sections

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