FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{Met-GAL4::p65}
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General Information
Symbol
P{Met-GAL4::p65}
FlyBase ID
FBtp0117974
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4p65.Met.CH321-28O05
Also Known As
Met-GAL4::p65
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4p65.Met.CH321-28O05
Transgenic product class
Component
Use(s)
Regulatory region(s)
Met
Encoded product / tool
GAL4::p65
Description

The GAL4::p65 driver is expressed under the control of Met regulatory sequences. Consists of the genomic BAC clone CH321-28O05 in which the first exon of Met has been replaced with a GAL4::p65 driver followed by expression termination sequences. There is a small deletion in sequence upstream of the Met promoter (within the upstream CG1703 gene).

(Baumann et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage -- prepupal stage P1
embryonic/larval corpus allatum
(Baumann et al., 2017)
embryonic/larval corpus cardiacum
(Baumann et al., 2017)
prothoracic gland
(Baumann et al., 2017)
wandering third instar larval stage
embryonic/larval epidermis
(Baumann et al., 2017)
histoblast
(Baumann et al., 2017)
embryonic/larval oenocyte
(Baumann et al., 2017)
larval somatic muscle cell
(Baumann et al., 2017)
embryonic/larval fat body
(Baumann et al., 2017)
embryonic/larval salivary gland
(Baumann et al., 2017)
larval central nervous system | restricted
(Baumann et al., 2017)
embryonic/larval mushroom body | intense
(Baumann et al., 2017)
medulla anlage
(Baumann et al., 2017)
lobula anlage
(Baumann et al., 2017)
lobula plate anlage
(Baumann et al., 2017)
larval PTTH neuron of pars lateralis
(Baumann et al., 2017)
larval corpus allatum innervating neuron CA-LP 1
(Baumann et al., 2017)
larval corpus allatum innervating neuron CA-LP 2
(Baumann et al., 2017)
photoreceptor neuron of eye disc
(Baumann et al., 2017)
genital disc
(Baumann et al., 2017)
wing disc
(Baumann et al., 2017)
prothoracic leg disc
(Baumann et al., 2017)
mesothoracic leg disc
(Baumann et al., 2017)
adult stage | male
ejaculatory duct
(Baumann et al., 2017)
ejaculatory bulb
(Baumann et al., 2017)
male accessory gland
(Baumann et al., 2017)
vas deferens
(Baumann et al., 2017)
adult stage | female
spermatheca | intense
(Baumann et al., 2017)
seminal receptacle
(Baumann et al., 2017)
uterus
(Baumann et al., 2017)
adult stage
main body follicle cell | intense
(Baumann et al., 2017)
adult brain | restricted
(Baumann et al., 2017)
adult mushroom body | intense
(Baumann et al., 2017)
adult pharyngeal nerve
(Baumann et al., 2017)
peptidergic neuron | faint
(Baumann et al., 2017)
ellipsoid body
(Baumann et al., 2017)
fan-shaped body

Comment: layers

(Baumann et al., 2017)
bulb
(Baumann et al., 2017)
nodulus 3 | intense
(Baumann et al., 2017)
nodulus 2
(Baumann et al., 2017)
nodulus 1
(Baumann et al., 2017)
lobula complex
(Baumann et al., 2017)
medulla
(Baumann et al., 2017)
lobula layer 1 | intense
(Baumann et al., 2017)
T5 neuron
(Baumann et al., 2017)
T4 neuron
(Baumann et al., 2017)
second optic chiasma
(Baumann et al., 2017)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
wing disc
(Baumann et al., 2017)
metathoracic leg disc
(Baumann et al., 2017)
larval central nervous system
(Baumann et al., 2017)
Additional Information
Statement
Reference

ScerGAL4p65.Met.CH321-28O05 drives strong expression in the adult mushroom bodies and is widely expressed across the compartments of the central complex. It is highly expressed in nodulus subunit III compared to the other subunits. It is also expressed across the optic lobe neuropils and has strong expression in the aborizations of T5 neurons in lobula layer 1.

(Baumann et al., 2017)
 
Marker for
Reflects expression of
Met
(Baumann et al., 2017)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
77133
w*; P{Met-GAL4::p65}attP40
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Met-GAL4
(Wu et al., 2021)
Met-GAL4::P65
(Wu et al., 2021)
Met-GAL4::p65
(Baumann et al., 2017)
P{Met-GAL4::p65}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (5)