Reference Report

Reference
Citation	Bhat, M.A., Philp, A.V., Glover, D.M., Bellen, H.J. (1996). Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II. Cell 87(6): 1103--1114. (Export to RIS)
FlyBase ID	FBrf0091018
Publication Type	Research paper
PubMed ID	8978614
PubMed Abstract	We have isolated a Drosophila gene, barren (barr), required for sister-chromatid segregation in mitosis. barr encodes a novel protein that is present in proliferating cells and has homologs in yeast and human. Mitotic defects in barr embryos become apparent during cycle 16, resulting in a loss of PNS and CNS neurons. Centromeres move apart at the metaphase-anaphase transition and Cyclin B is degraded, but sister chromatids remain connected, resulting in chromatin bridging. This phenotype is similar to that described in TOP2 mutants in yeast. Barren protein localizes to chromatin throughout mitosis. Colocalization and biochemical experiments indicate that Barren associates with Topoisomerase II throughout mitosis and alters the activity of Topoisomerase II. We propose that this association is required for proper chromosomal segregation by facilitating the decatenation of chromatids at anaphase.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (5)
	Df(2L)barr^k04805 Df(2L)barr^k04912 Df(2L)pr-A16 Df(2L)TE38A-1 Df(2L)TW50
Alleles (10)
	barr^hs.PB barr^k00310 barr^k04805 barr^k04912 barr^k13903 barr^k14014 barr^k14028 barr^L305 neb^k00802 w^+mC
Constructs (1)
	P{CaSpeR-hs-barr}
Genes (12)
	anon-TE7 ato barr cpo CycB neb pros Top2 Trl w α-Spec αTub84B
Insertions (6)
	P{?'lacW}barr^L305 P{lacW}barr^k00310 P{lacW}barr^k04912 P{lacW}barr^k13903 P{lacW}barr^k14014 P{lacW}barr^k14028
Transcripts (1)
	barr-RA