Heterozygous dsh6 mutant flies show dorsal cluster neuron branching defects in the adult medulla; the number of branches at the 3rd branching point of the grid-like structure is significantly increased.
The average number of dorsal cluster neurons that cross between the lobula and the medulla is reduced from the wild-type number of 11.7 to 7.6 in the brains of dsh6/+ mutants.
Homozygous larvae grown at the restrictive temperature result in heads that lack both the lateral and mediolateral structures causing the ocelli to be directly adjacent to the compound eyes. Ocellar cuticle and the ocelli are larger than wild type.
Hemizygotes lack antennal structures and have bulbous, truncated legs. Legs show a deletion of ventral markers replaced with a duplication of dorsal pattern elements. 20% of mutants are lacking one, or both wings and have a duplicated notum.
Females possessing homozygous germline clones when mated to wild type males produce two classes of embryos: heterozygous females are fertile and morphogenetically normal and hemizygous male embryos lack dorsal cuticle, posterior spiracles and filzkorper material. The ventral cuticle present has no naked cuticle in the thoracic or abdominal segments. Embryos show an aberrant pattern of cell death and lack of parasegmental and segmental boundaries.