FlyBase Allele Report: Dmel\elav[5]

General Information

Symbol

Dmel\elav⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0003634

Feature type

allele

Associated gene

Dmel\elav

Associated Insertion(s)

Carried in Construct

Also Known As

elav^e5

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Samson et al., 1995)

loss of function allele

(Simionato et al., 2007, Koushika et al., 1996)

Mutagen

X ray

Progenitor genotype

Cytology

Description

Interstitial deletion within elav.

(Koushika et al., 1996)

Deletion of the entire protein coding region.

(Robinow and White, 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The neuromuscular junctions of elav⁵/elav^ts1 mutant third instar larvae (raised at the permissive temperature during embryonic development) show a strong reduction in the number of synaptic connections compared with controls.

elav⁵/elav^ts1 adult flies do not exhibit any fusion of the mushroom body beta lobes.

elav⁵ mutant flies exhibit loss of photoreceptors and central brain neurons.

elav⁵/elav^ts1 are short lived, show reduced locomotion and are ataxic.

elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium) are small compared with controls.

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals show a significant reduction in the number of type 1b boutons at the neuromuscular junction of muscle 13 when they are reared at the restrictive temperature during larval life.

(Haussmann et al., 2008)

elav⁵ mutants affect commissure formation in all neuromeres and lead to a reduction in the number of commissural fibers crossing the CNS midline. The commissural fibers are thinner than in wild-type in 95% of the neuromeres. The posterior commissure is missing entirely in 25% of the neuromeres and both commissures are absent in 5% of the neuromeres. A greater distance between the longitudinal tracts is observed in more than 80% of the neuromeres.

In elav⁵ mutant embryos, EW commissural axons frequently fail to cross the midline and instead grow ipsilaterally (80% of the neuromeres). Similarly, but to a lesser extent, EG commissural axons frequently do not cross the midline, preferentially extending on their own side (>50% of the neuromeres). Contralateral projections were found impaired in 30% of the neuromeres for the SP neurons and no contralateral projections are seen for the RP3 neurons.

Midline glial cells are specified normally and are present in normal numbers and have normal position and morphology in elav⁵ mutant embryos.

(Simionato et al., 2007)

elav⁵ elav^edr flies have rough eyes and a disorganised, vacuolar retina.

(Koushika et al., 1996)

elav⁵ flies carrying one copy of elav^RBD have reduced viability and abnormalities in the eye and optic lobes.

(Yao et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

elav⁵ has abnormal neuroanatomy phenotype, enhanceable by comm⁵/comm[+]

(Simionato et al., 2007)

elav⁵ has abnormal neuroanatomy phenotype, enhanceable by sli²/sli[+]

(Simionato et al., 2007)

NOT Enhanced by

Statement

Reference

elav⁵/elav^ts1 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Rbp9^P2690/Df(2L)ED206

(Zaharieva et al., 2015)

elav⁵ has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(1)fne^Δ

(Zaharieva et al., 2015)

elav⁵ has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Rbp9^P2690

(Zaharieva et al., 2015)

elav⁵ has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Rbp9^P2690/Df(1)fne^Δ

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Df(1)fne^Δ

(Zaharieva et al., 2015)

Suppressed by

Statement

Reference

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has abnormal neuroanatomy | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵ has visible | somatic clone phenotype, suppressible by Scer\GAL4^elav.PZ/fne^UAS.Tag:HA

(Zaharieva et al., 2015)

elav⁵ has visible | somatic clone phenotype, suppressible by Rbp9^UAS.Tag:HA/Scer\GAL4^elav.PZ

(Zaharieva et al., 2015)

elav⁵ has visible | somatic clone phenotype, suppressible by Sxl^UAS.cHa/Scer\GAL4^elav.PZ

(Zaharieva et al., 2015)

elav⁵ has visible | somatic clone phenotype, suppressible by Scer\GAL4^elav.PZ/Hsap\ELAVL1^UAS.Tag:HA

(Zaharieva et al., 2015)

elav⁵ has lethal phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has lethal | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵/elav^ts1 has abnormal neuroanatomy | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵ has abnormal neuroanatomy phenotype, suppressible by robo[+]/robo1¹

(Simionato et al., 2007)

elav⁵ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^eg-Mz360/comm^UAS.cWa

(Simionato et al., 2007)

elav⁵ has lethal phenotype, suppressible by elav::Rbp9^ER3

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, suppressible by elav::Sxl^ES1.2

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, suppressible by elav::Sxl^ES2

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, suppressible by elav::Rbp9^ER1

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, suppressible by elav::Hsap\ELAVL4^EH3

(Lisbin et al., 2000)

NOT suppressed by

Statement

Reference

elav⁵ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^eg-Mz360/fra^UAS.cKa

(Simionato et al., 2007)

elav⁵ has lethal phenotype, non-suppressible by elav::Rbp9^ER12

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, non-suppressible by elav::Sxl^ES12

(Lisbin et al., 2000)

elav⁵ has lethal phenotype, non-suppressible by elav::Sxl^ES1

(Lisbin et al., 2000)

Enhancer of

Statement

Reference

elav⁵ is an enhancer of abnormal neuroanatomy phenotype of comm⁵

(Simionato et al., 2007)

Suppressor of

Statement

Reference

elav⁵ is a suppressor of abnormal neuroanatomy phenotype of robo1¹

(Simionato et al., 2007)

elav⁵ is a suppressor of abnormal neuroanatomy phenotype of sli²

(Simionato et al., 2007)

Other

Statement

Reference

Df(1)fne^Δ, elav⁵/elav^ts1 has lethal - all die before end of pupal stage phenotype

(Zaharieva et al., 2015)

Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die before end of pupal stage phenotype

(Zaharieva et al., 2015)

Df(1)fne^Δ, Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die during embryonic stage phenotype

(Zaharieva et al., 2015)

elav⁵/elav^ts1, ewg^SC3ORF.elav has flightless phenotype

(Haussmann et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

elav⁵ has symmetrical commissure phenotype, enhanceable by comm⁵/comm[+]

(Simionato et al., 2007)

elav⁵ has connective phenotype, enhanceable by comm⁵/comm[+]

(Simionato et al., 2007)

elav⁵ has symmetrical commissure phenotype, enhanceable by sli²/sli[+]

(Simionato et al., 2007)

NOT Enhanced by

Statement

Reference

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Df(1)fne^Δ

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Rbp9^P2690/Df(2L)ED206

(Zaharieva et al., 2015)

Suppressed by

Statement

Reference

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by Rbp9^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | third instar larval stage | conditional phenotype, suppressible by Hsap\ELAVL1^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵ has eye | somatic clone phenotype, suppressible by Scer\GAL4^elav.PZ/fne^UAS.Tag:HA

(Zaharieva et al., 2015)

elav⁵ has eye | somatic clone phenotype, suppressible by Rbp9^UAS.Tag:HA/Scer\GAL4^elav.PZ

(Zaharieva et al., 2015)

elav⁵ has eye | somatic clone phenotype, suppressible by Sxl^UAS.cHa/Scer\GAL4^elav.PZ

(Zaharieva et al., 2015)

elav⁵ has eye | somatic clone phenotype, suppressible by Scer\GAL4^elav.PZ/Hsap\ELAVL1^UAS.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has embryonic/larval neuromuscular junction | third instar larval stage | conditional phenotype, suppressible by fne^elav.Tag:HA

(Zaharieva et al., 2015)

elav⁵/elav^ts1 has NMJ bouton | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵/elav^ts1 has neuromuscular junction | heat sensitive phenotype, suppressible by ewg^SC3ORF.elav

(Haussmann et al., 2008)

elav⁵ has symmetrical commissure phenotype, suppressible by robo[+]/robo1¹

(Simionato et al., 2007)

elav⁵ has symmetrical commissure phenotype, suppressible by Scer\GAL4^eg-Mz360/comm^UAS.cWa

(Simionato et al., 2007)

elav⁵ has phenotype, suppressible by elav::Dvir\elav^DvORF

(Samson, 1998)

NOT suppressed by

Statement

Reference

elav⁵ has symmetrical commissure phenotype, non-suppressible by Scer\GAL4^eg-Mz360/fra^UAS.cKa

(Simionato et al., 2007)

Enhancer of

Statement

Reference

elav⁵ is an enhancer of symmetrical commissure phenotype of comm⁵

(Simionato et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The axon wiring defects seen in elav⁵ mutant embryos is not enhanced by Df(1)fne^Δ or Rbp9^P2690, or both.

Df(1)fne^Δ does not enhance the neuromuscular junction phenotypes seen in elav⁵/elav^ts1 mutant third instar larvae.

Rbp9^P2690/Df(2L)ED206 does not enhance the neuromuscular junction phenotypes seen in elav⁵/elav^ts1 mutant third instar larvae.

Expression of fne^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PZ rescues the small eye phenotype seen in elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium).

Expression of Rbp9^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PZ rescues the small eye phenotype seen in elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium).

Expression of Sxl^Scer\UAS.cHa under the control of Scer\GAL4^elav.PZ does not rescue the small eye phenotype seen in elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium).

Expression of fne^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

Expression of Rbp9^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

Expression of Rbp9^{elav.T:Ivir\HA1} does not rescue the viability of elav⁵.

Expression of Rbp9^{elav.T:Uuuu\nls-Unk.T:Ivir\HA1} does not rescue the viability of elav⁵.

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals eclose as adults when they are rescued by ewg^elav.NS (providing that embryogenesis occurs at the permissive temperature for the elav^ts1 allele). However, the rescued adults show motor defects and are flightless.

The reduction in bouton number at the neuromuscular junction which is seen in elav^ts1/elav⁵ animals reared at the restrictive temperature during larval life is rescued by ewg^elav.NS.

(Haussmann et al., 2008)

elav⁵ mutants that are heterozygous for comm⁵ exhibit a comm-like phenotype, whereas comm⁵/+ embryos display no abnormalities. In elav⁵; comm⁵/+ embryos commissures are missing entirely in more than 80% of the neuromeres, indicating that most commissural axons fail to cross the midline in these embryos. The longitudinal tracts frequently adopt a more lateral position (i.e. higher distance from the midline) relative to elav⁵ embryos.

Reduction of robo in a robo¹ heterozygous background partially suppresses the elav⁵ mutant commissural phenotype. Thicker commissural tracts (relative to elav⁵ embryos) are made in more than 50% of the neuromeres.

Reduction of sli in a sli² heterozygous background leads to a fused commissures phenotype in elav⁵ mutant embryos.

Expression of comm^Scer\UAS.cWa under the control of Scer\GAL4^eg-Mz360 in elav⁵ mutant EW and EG neurons suppresses the elav⁵ phenotype, with these neurons once again crossing the midline to generate commissures.

Expression of fra^Scer\UAS.cKa in elav⁵ mutant EW and EG neurons (under the control of Scer\GAL4^eg-Mz360) does not modify the proportion of contralateral projection defects in elav⁵ mutant embryos. For example, EW axons fail to cross the midline in 755 of the neuromeres in such embryos.

(Simionato et al., 2007)

Two transgenic lines of elav::Rbp9^ER1 and one of elav::Sxl^ES2 show slight rescue of elav⁵ (4, 6 and 5% rescue respectively).

(Lisbin et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ELAVL1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PZ rescues the small eye phenotype seen in elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium).

Expression of Hsap\ELAVL1^{elav.T:Ivir\HA1} slightly rescues the viability of elav⁵.

Expression of Hsap\ELAVL1^{elav.T:Ivir\HA1} rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant larvae (raised at the permissive temperature during embryonic development).

(Zaharieva et al., 2015)

Complementation and Rescue Data

Rescued by

elav⁵/elav^ts1 is rescued by elav^Tag:HA

(Zaharieva et al., 2015)

elav⁵ is rescued by elav^{UAS.Tag:HA.cZa}/Scer\GAL4^elav.PZ

(Zaharieva et al., 2015)

elav⁵/elav^ts1 is rescued by elav^DmORF

(Haussmann et al., 2008)

elav⁵ is rescued by Scer\GAL4^e13C/elav^UAS.cFa

(Simionato et al., 2007)

elav⁵ is rescued by elav^DmORF

(Lisbin et al., 2000)

elav⁵ is rescued by elav^-13

(Lisbin et al., 2000)

elav⁵ is rescued by elav^DmORF

(Samson, 1998)

elav⁵ is rescued by elav³³⁹

(Samson, 1998)

elav⁵ is rescued by elav^Δ5'int

(Samson, 1998)

elav⁵ is rescued by elav^Δint

(Samson, 1998)

elav⁵ is rescued by elav^+t15.5

(Samson, 1998)

elav⁵ is rescued by elav^+t13.5

(Samson, 1998)

elav⁵ is rescued by elav^DmORF

(Koushika et al., 1996)

Partially rescued by

elav⁵ is partially rescued by elav^ΔOh

(Yannoni and White, 1999)

elav⁵ is partially rescued by elav^DmORF

(Yannoni and White, 1999)

elav⁵ is partially rescued by elav^ΔCh

(Yannoni and White, 1999)

elav⁵ is partially rescued by elav^{ΔCh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav⁵ is partially rescued by elav^{ΔOh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav⁵ is partially rescued by elav^20.t15.5

(Samson, 1998)

elav⁵ is partially rescued by elav^RBD

(Yao et al., 1993)

Not rescued by

elav⁵ is not rescued by elav^3AGD

(Lisbin et al., 2000)

elav⁵ is not rescued by elav^2AGD

(Lisbin et al., 2000)

elav⁵ is not rescued by elav^1AGD

(Lisbin et al., 2000)

elav⁵ is not rescued by elav^{ΔNh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav⁵ is not rescued by elav^ΔNh

(Yannoni and White, 1999)

elav⁵ is not rescued by elav^{ΔEh.Tag:NLS(PV-largeT)}

(Yannoni and White, 1999)

elav⁵ is not rescued by elav^ΔEh

(Yannoni and White, 1999)

elav⁵ is not rescued by elav^19.tSa

(Samson, 1998)

elav⁵ is not rescued by elav^20.tSa

(Samson, 1998)

elav⁵ is not rescued by elav³³⁶

(Samson, 1998)

elav⁵ is not rescued by elav^ts1.tSa

(Samson, 1998)

Comments

Expression of elav^{Scer\UAS.T:Ivir\HA1.cZa} under the control of Scer\GAL4^elav.PZ rescues the small eye phenotype seen in elav⁵ mutant eyes (generated by expressing elav^elav.PZ and excising the FRT cassette in the eye primordium).

Expression of elav^T:Ivir\HA1 rescues the reduction in bouton number seen in elav^ts1/elav⁵ mutant flies (raised at the permissive temperature during embryonic development).

(Zaharieva et al., 2015)

elav^ts1/elav⁵ animals eclose as adults when they are rescued by elav^DmORF (providing that embryogenesis occurs at the permissive temperature for the elav^ts1 allele).

The reduction in bouton number at the neuromuscular junction which is seen in elav^ts1/elav⁵ animals reared at the restrictive temperature during larval life is rescued by elav^DmORF.

(Haussmann et al., 2008)

Expression of elav^Scer\UAS.cKa in en-expressing neurons (under the control of Scer\GAL4^e13C) in elav⁵ mutant embryos restores formation of en-expressing posterior commissures (as in wild-type).

(Simionato et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

77877

elav⁵ w^* sn^*/FM6, l(1)*^*/Dp(1;Y)y⁺sc

Notes on Origin

Discoverer

Lefevre.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

elav⁵

(Zhang et al., 2023, Zhang et al., 2023, Wei et al., 2020, Kavaler et al., 2018, Simionato et al., 2007)

elav^e5

(Zaharieva et al., 2015, Haussmann et al., 2008, Toba and White, 2008, Soller and White, 2003, Lisbin et al., 2001, Koushika et al., 2000, Lisbin et al., 2000, Samson, 2000, Yannoni and White, 1999, Samson, 1998, Yannoni and White, 1997, Koushika et al., 1996, Samson et al., 1995, Yao et al., 1993, Robinow and White, 1991, Yao and White, 1991)

l(1)A138

Name Synonyms

Secondary FlyBase IDs

References (21)

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