FlyBase Allele Report: Dmel\pb[4]

General Information

Symbol

Dmel\pb⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0013508

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Benassayag et al., 1997, Benassayag et al., 1997, Cribbs et al., 1995, Cribbs et al., 1992, Randazzo et al., 1991)

loss of function allele

(Pultz et al., 1988)

Mutagen

gamma ray

Progenitor genotype

Cytology

Polytene chromosomes normal.

Description

Coordinates of the lesion are the position in the 2-μ-4a spliced form of pb.

(Tayyab et al., 2004)

Nucleotide substitution: C1819T.

(Tayyab et al., 2004)

Amino acid replacement: Q607term.

(Tayyab et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,709,698..6,709,698 [-]

Nucleotide change:

C6709698T

Reported nucleotide change:

C1819T

Amino acid change:

Q607term | pb-PA; Q602term | pb-PB; Q602term | pb-PC; Q597term | pb-PD

Reported amino acid change:

Q607term

Comment:

Reported coordinates are with respect to pb-RA and pb-PA.

(Tayyab et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible (with pb²⁰)

(Tayyab et al., 2004)

visible | recessive

(Boube et al., 1998, Benassayag et al., 1997)

Phenotype Manifest In

arista | ectopic (with pb⁵)

(Calgaro et al., 2002, Boube et al., 2000)

arista | ectopic (with pb²⁰)

(Tayyab et al., 2004)

labellar cap (with pb²⁰)

(Tayyab et al., 2004)

labellum

(Benassayag et al., 1997, Boube et al., 1997, Cribbs et al., 1992)

labellum (with pb⁵)

(Boube et al., 1997)

labellum | distal

(Boube et al., 1998)

labellum | distal (with pb⁵)

(Calgaro et al., 2002)

labial segment (with pb⁵)

(Boube et al., 2000)

maxillary palp (with pb²⁰)

(Tayyab et al., 2004)

pseudotrachea

(Lewis et al., 1980)

pseudotrachea (with pb⁵)

(Boube et al., 2000)

pseudotrachea (with pb¹³)

(Calgaro et al., 2002)

pseudotrachea (with pb²⁰)

(Tayyab et al., 2004)

Detailed Description

Statement

Reference

pb⁴/pb²⁰ flies show a reduction in length of the maxillary palps, the labellar cap is transformed to an arista and the number of pseudotracheal rows is reduced to 1 or 2.

(Tayyab et al., 2004)

pb⁴/pb¹³ flies have nearly normal labial palps, which show a slight reduction of the pseudotracheal rows. pb⁴/pb⁵ flies show a transformation of distal labium to antennal arista.

(Calgaro et al., 2002)

pb⁴/pb⁵ flies have a transformation of the distal labium to antennal arista, with a concomitant reduction of the pseudotrachea.

(Boube et al., 2000)

Flies show transformation of the distal labial palps to antennal aristae. Some pseudotracheal tissue remains on the proximo-medial part of each palp.

(Boube et al., 1998)

Homozygotes show a partial transformation of the labial palps to antennal aristae.

(Benassayag et al., 1997)

Homozygotes show transformation of the labium to aristal structures. pb⁴/pb⁵ flies show mixed transformation of the distal labium to leg and aristal structures.

(Boube et al., 1997)

The labial palp is transformed to antenna.

(Cribbs et al., 1992)

Hemizygotes have transformations that range from a predominantly antennal morphology to a mixture of antennal-like and leg-like characteristics.

(Pultz et al., 1988)

Transformation of the first three pseudotracheal rows of the labial palps to antenna or leg identity.

(Lewis et al., 1980)

antenna

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pb⁵/pb⁴ has labial segment phenotype, enhanceable by skd^pap-1

(Boube et al., 2000)

pb⁵/pb⁴ has pseudotrachea phenotype, enhanceable by skd^pap-1

(Boube et al., 2000)

pb⁵/pb⁴ has labial segment phenotype, enhanceable by skd^pap-1/MED17^s2956

(Boube et al., 2000)

pb⁵/pb⁴ has pseudotrachea phenotype, enhanceable by skd^pap-1/MED17^s2956

(Boube et al., 2000)

pb⁵/pb⁴ has labellum phenotype, enhanceable by Df(3R)ΔC40b

(Boube et al., 1997)

Suppressed by

Statement

Reference

pb⁴ has labellum phenotype, suppressible by RasGAP1^sxt-BJ61

(Boube et al., 1997)

Other

Statement

Reference

pb⁴/pb¹³, tara^L4 has labellum | distal phenotype

(Calgaro et al., 2002)

pb⁴/pb¹³, tara^L4 has arista | ectopic phenotype

(Calgaro et al., 2002)

pb⁴/pb¹³, tara^L4/tara[+] has labellum | distal phenotype

(Calgaro et al., 2002)

pb⁴/pb¹³, tara^L4/tara[+] has arista | ectopic phenotype

(Calgaro et al., 2002)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has sex comb | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has unguis | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has sex comb | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has unguis | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has sex comb | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has unguis | ectopic phenotype

(Boube et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

pb⁴ tara^L4/pb¹³ + flies show a weak but reliable transformation of the distal labium to antennal arista.

(Calgaro et al., 2002)

The addition of a combination of skd^pap-1 and MED17^s2956 enhances the transformation phenotype seen in the labium of pb⁴/pb⁵ flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including sex comb teeth and claws) is seen. The addition of skd^pap-1 enhances the transformation phenotype seen in the labium of pb⁴/pb⁵ flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including claws) is seen.

(Boube et al., 2000)

The transformation of the labium to aristal structures seen in pb⁴ homozygotes is altered towards wild-type if the flies also carry one copy of Gap1^sxt-BJ61. pb⁴/pb⁵ flies also carrying one copy of Df(3R)ΔC40b generally have a more severe labial palp mutant phenotype, including the appearance of prothorax-specific sex comb teeth and distal claws.

(Boube et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pb⁴ is rescued by pb^hs.PB

(Benassayag et al., 1997)

pb⁴ is rescued by pb^hs.PB

(Benassayag et al., 1997)

pb⁴ is rescued by pb^hs.PB

(Cribbs et al., 1995)

Partially rescued by

pb⁴ is partially rescued by pb^Rev2.HSPB

(Benassayag et al., 1997)

pb⁴ is partially rescued by pb^Rev19.HSPB

(Benassayag et al., 1997)

pb⁴ is partially rescued by pb^Rev20.HSPB

(Benassayag et al., 1997)

Not rescued by

pb⁴ is not rescued by pb^Rev5.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev14.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev1.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev23.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev9.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev3.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev17.HSPB

(Benassayag et al., 1997)

pb⁴ is not rescued by pb^Rev3.HSPB

(Benassayag et al., 1997)

Comments

The labial palp phenotype is fully rescued by pb^hs.PB, is not rescued by pb^Rev9.HSPB, pb^Rev14.HSPB, pb^Rev23.HSPB, pb^Rev1.HSPB, pb^Rev5.HSPB, pb^Rev17.HSPB or pb^Rev3.HSPB, and is partially rescued by pb^Rev2.HSPB, pb^Rev19.HSPB or pb^Rev20.HSPB.

(Benassayag et al., 1997)

The labium to antenna phenotype of pb⁴ flies can be rescued by pb^hs.PB.

(Cribbs et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

2112

Ki¹ pb⁴ p^p ss^a40a/TM2

2108

Diap1^th-1 st¹ Ki¹ pb⁴ p^p/TM3, Sb¹

2111

In(3R)Antp^73b, Ki¹ pb⁴ Antp^73b sas^Antp73b ss^a/TM3, Sb¹

Kyoto

106666

In(3R)Antp^73b, Ki¹ pb⁴ Antp^73b sas^Antp73b ss^a/TM3, Sb¹

Notes on Origin

Discoverer

Duncan and Kaufman.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

(Cribbs et al., 1992)

pb⁴

(Tayyab et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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