FlyBase Allele Report: Dmel\trx[E3]

General Information

Symbol

Dmel\trx^E3

Species

D. melanogaster

Name

FlyBase ID

FBal0017175

Feature type

allele

Associated gene

Dmel\trx

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Breen, 1999)

Mutagen

ethyl methanesulfonate

(Kennison and Tamkun, 1988)

gamma ray

(Breen and Harte, 1991)

Progenitor genotype

Cytology

Description

trx^E3 is associated with an in-frame deletion that removes 271 amino acids from both TRXI and TRXII isoforms. The deleted residues are located on the C-terminal side of the central cysteine-rich domain that contains PHD fingers.

(Breen, 1999)

Deletion of a 280 amino acid domain, domain E3.

(Kuzin et al., 1994)

700bp deletion within coding region.

(Breen and Harte, 1991)

Deletion of 832bp DNA and insertion of 19 nucleotides, resulting in a deletion of 271 amino acids.

(Mazo et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:14,269,094..14,269,925 [-]

Comment:

Deletion of 832 nucleotides and insertion of 19 nucleotides leads to an in frame deletion of 271 amino acids.

(Mazo et al., 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal tergite 1

(Breen, 1999)

abdominal tergite 2

(Breen, 1999)

abdominal tergite 3

(Breen, 1999)

abdominal tergite 5 | male

(Breen, 1999)

abdominal tergite 6 | male

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

larval brain | larval stage (with trx^B11)

(Muyrers-Chen et al., 2004)

metathoracic laterotergite

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

pupal cuticle & abdomen

(Breen, 1999)

sex comb

(Breen, 1999)

Detailed Description

Statement

Reference

The brains of trx^E3/trx^B11 larvae show a severe reduction in BrdU incorporation compared to wild type. Brain squashes are characterised by global chromatin decondensation, resembling 'prophase-like' chromatin.

(Muyrers-Chen et al., 2004)

1.6% of heterozygous adults show at least one transformation phenotype. Hemizygotes are recessive lethal dying at the pupal stage, homozygotes are embryonic lethal. trx^E3/Df(3R)red-P52 hemizygotes show phenotypes that suggest decreased Scr function (transformation of T1 structures to a T2 identity), decreased Ubx function (transformation of T3 to T2), decreased abd-A function (transformation of A2-A7 to A1) and decreased Abd-B function (transformation of posterior abdominal segments to more anterior abdominal identities). Scr group transformations include the development of mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the prothoracic tibia, reduced numbers of sex comb teeth (in males), development of sternopleural bristles on the T1 segment. Ubx group transformations include haltere to wing, metathoracic laterotergite to scutum, the development of T3 sternopleural bristles, mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the metathoracic tibia. abd-A group transformations include, the development of A1 type bristles on abdominal tergites 2 to 3 Abd-B group transformations include, the pigmentation of abdominal tergites 5 to 6 in males, and enlargement of abdominal tergite 7. Flies transheterozygous for trx^E3 and a strong trx hypomorph, especially, trx^Z16, frequently have missing dorsal head structures, including different combinations of ocelli, ocellar bristles, and postvertical bristles. The posterior border of the A1 tergite may have Uab-like bristles and dark pigmentation. They can also exhibit incomplete dorsal fusion of tergites. The dorsal anal plate of females may be incomplete. Many trx^E3 hemizygotes develop as incomplete pharate adults whose heads fail to evert (and appear headless). They also have incomplete chitinisation.

(Breen, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

trx^E3/trx[+] is a suppressor of visible | homeotic phenotype of Antp^Ns, Pc²

(Kennison and Tamkun, 1988)

Phenotype Manifest In

Suppressor of

Statement

Reference

trx^E3/trx[+] is a suppressor of antenna phenotype of Antp^Ns, Pc²

(Kennison and Tamkun, 1988)

trx^E3/trx[+] is a suppressor of sex comb phenotype of Antp^Scx, Pc²

(Kennison and Tamkun, 1988)

Additional Comments

Genetic Interactions

Statement

Reference

Suppresses the antenna to leg transformation phenotype of Pc², Antp^Ns/+. Causes between 50% and 100% suppression of the Pc⁴/+ extra sex combs phenotype.

(Kennison and Tamkun, 1988)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

(Breen, 1999)

Comments

Partial complementation between hypomorphs produced phenotypes that were weaker than either of the hemizygous or homozygous phenotypes, either rescuing lethality or producing a weaker homeotic transformation phenotype. Transformations range from an average of 0.12 to 7.90 transformations/fly (males were scored for a possible 29 transformations, females were scored for a possible 26 transformations) in different allelic combinations.

(Breen, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The following alleles form a series of progressively increasing penetrance and expressivity of phenotype when in transheterozygous combination with trx¹ : trx^M17 > trx^Z32 > trx^Z16 > trx^Z11 > trx^M18 = trx^JY16 > trx^E3.

(Breen, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trx^E3

(Muyrers-Chen et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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