FlyBase Allele Report: Dmel\cic[08482]

(Rodríguez-Muñoz et al., 2022, Andersen and Horne-Badovinac, 2016, Jimenez et al., 2000, Spradling et al., 1999, Rittenhouse and Berg, 1995, BDGP Project Members, 1994-1999, Rittenhouse and Berg, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Berg, 1994, Rittenhouse and Berg, 1994)

female sterile (with Df(3R)Delta-BX12)

(Rittenhouse and Berg, 1995)

female sterile | recessive

(Spradling et al., 1999)

female sterile germline-dependent

(Rittenhouse and Berg, 1995)

visible (with Df(3R)Delta-BX12)

(Rittenhouse and Berg, 1995)

Phenotype Manifest In

dorsal appendage

(Rittenhouse and Berg, 1995, Berg, 1994)

dorsal appendage, with bwk¹⁵¹

(Dorman et al., 2004, Tran and Berg, 2003)

egg chamber (with cic⁷)

(Rodríguez-Muñoz et al., 2022)

egg chorion

(Rittenhouse and Berg, 1995)

egg chorion (with Df(3R)Delta-BX12)

(Rittenhouse and Berg, 1995)

egg chorion | anterior

(Spradling, 1993)

embryo

(Rittenhouse and Berg, 1995, Berg, 1994, Rittenhouse and Berg, 1994)

embryo, with bwk¹⁵¹

(Rittenhouse and Berg, 1995)

follicle cell

(Rittenhouse and Berg, 1995, Rittenhouse and Berg, 1994)

wing (with Df(3R)Delta-BX12)

(Rittenhouse and Berg, 1995)

wing vein | ectopic, with bwk^Δ4

(Roch et al., 2002)

wing vein | ectopic, with bwk^Δ11

(Roch et al., 2002)

wing vein | ectopic, with bwk^Δ14

(Roch et al., 2002)

wing vein | ectopic, with bwk^Δ81

(Roch et al., 2002)

wing vein | ectopic (with cic^D49)

(Roch et al., 2002)

Detailed Description

Statement

Reference

Stages 10B and early 11 of dorsal appendage morphogenesis proceed normally in bwk¹⁵¹/bwk⁰⁸⁴⁸² egg chambers in culture. However, defects are seen in later stages. Anterior extension of the developing dorsal appendage often occurs abnormally; although it may begin normally, the dorsal appendage forming cells do not advance to the anterior of the egg chamber, the two dorsal appendages are often asymmetrical and the lumens are often either bent or expanded. Cultured egg chambers also show other defects including rounded-up and delaminating follicle cells. bwk¹⁵¹/bwk⁰⁸⁴⁸² dorsal appendages have an abnormally wide "moose-antler" shape.

(Dorman et al., 2004)

bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs have mainly short, broad dorsal appendages.

(Tran and Berg, 2003)

bwk⁰⁸⁴⁸² combined with bwk^Δ11, bwk^Δ81, bwk^Δ4 and bwk^Δ11 produces animals with an extra veins phenotype.

(Roch et al., 2002)

Homozygous females lay few eggs. Eggs often appear deflated, are shorter than wild type due to incomplete transfer of nurse cell contents into the oocyte (dumpless phenotype) and they exhibit a number of anterior eggshell defects. The dorsal eggshell filaments are short and broad and have ragged edges and thin chorion (sometimes resembling moose antlers). Phenotype is caused by aberrant follicle cell migration, not follicle cell fate changes or the dumpless phenotype. bwk⁰⁸⁴⁸²/bwk¹⁵¹ mothers produce many bicaudal embryos. Homozygous females lay few eggs. Eggs often appear deflated, are shorter than wild type due to incomplete transfer of nurse cell contents into the oocyte (dumpless phenotype) and they exhibit a number of anterior eggshell defects. The dorsal eggshell filaments are short and broad and have ragged edges and thin chorion (sometimes resembling moose antlers). Phenotype is caused by aberrant follicle cell migration, not follicle cell fate changes or the dumpless phenotype. Egg chambers from grk²; bwk⁰⁸⁴⁸² females have single dorsal filaments, as for grk² homozygotes, but they resemble bwk filaments, short and ragged. Homozygous embryos are bicaudal. bwk⁰⁸⁴⁸²/bwk¹⁵¹ mothers produce many bicaudal embryos. Flies in trans with Df(3R)Dl-BX12 exhibit sterility and have similar egg shell defects to bwk⁰⁸⁴⁸² homozygotes. In addition these flies have blistered wings.

(Rittenhouse and Berg, 1995)

Eggs have moose-antler-like dorsal appendages. Embryos are bicaudal.

(Berg, 1994)

In homozygous females the follicle cells that form the dorsal appendages do not migrate properly resulting in abnormal dorsal appendages that resemble moose antlers. Embryos are bicaudal.

(Rittenhouse and Berg, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by bsk^UAS.cBa/Scer\GAL4^c415

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by Scer\GAL4^CY2/Shark^UAS.cFa

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by sax[+]/sax⁵

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by dpp¹⁰⁶³⁸/dpp[+]

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by bsk²/bsk[+]

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-enhanceable by tkv⁷/tkv[+]

(Tran and Berg, 2003)

Suppressed by

Statement

Reference

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, suppressible | partially by Scer\GAL4^c415/Shark^UAS.cFa

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, suppressible | partially by Scer\GAL4^55B/Shark^UAS.cFa

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, suppressible by Scer\GAL4^c415/Src42A^CA.UAS

(Tran and Berg, 2003)

NOT suppressed by

Statement

Reference

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by bsk^UAS.cBa/Scer\GAL4^c415

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by Scer\GAL4^CY2/Shark^UAS.cFa

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by sax[+]/sax⁵

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by dpp¹⁰⁶³⁸/dpp[+]

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by bsk²/bsk[+]

(Tran and Berg, 2003)

bwk¹⁵¹, cic⁰⁸⁴⁸² has dorsal appendage phenotype, non-suppressible by tkv⁷/tkv[+]

(Tran and Berg, 2003)

cic⁰⁸⁴⁸² has phenotype, non-suppressible by cic^+tJa

(Jimenez et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is partially suppressed by the expression of shark^Scer\UAS.cFa under the control of Scer\GAL4^c415 or Scer\GAL4^55B. Expression of shark^Scer\UAS.cFa under the control of Scer\GAL4^CY2 has little effect on the mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs. The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is not significantly modified by bsk²/+, dpp¹⁰⁶³⁸/+, sax⁵/+ or tkv⁷/+. The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is not significantly modified by expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^c415. The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is suppressed by expression of Src42A^CA.Scer\UAS under the control of Scer\GAL4^c415.

(Tran and Berg, 2003)

bwk⁰⁸⁴⁸² combined with cic¹ produces wild-type animals. bwk⁰⁸⁴⁸² combined with cic^D49 produces animals with an extra veins phenotype. cic^{+tJa.T:Ivir\HA1} significantly reduces the extravein phenotype seen in cic^D49/bwk⁰⁸⁴⁸² animals, but not the chorion phenotype of eggs laid by bwk⁰⁸⁴⁸² mothers.

(Roch et al., 2002)

Egg chambers from grk²; bwk⁰⁸⁴⁸² females have single dorsal filaments, as for grk² homozygotes, but they resemble bwk filaments, short and ragged. Homozygous embryos are bicaudal.

(Rittenhouse and Berg, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

cic¹

(Roch et al., 2002)

Rescued by

cic⁰⁸⁴⁸²/cic⁷ is rescued by cic^L.RD.Tag:HA

(Rodríguez-Muñoz et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10309

ry⁵⁰⁶ P{PZ}cic⁰⁸⁴⁸²/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Complements: cic¹.

(Jimenez et al., 2000)

Excision alleles indicate bwk is important for other developmental processes: some lines are pupal lethal (flies never emerge from the pupal case) and one line has an extra wing vein phenotype.

(Rittenhouse and Berg, 1994)

Excision of the P{PZ} element reverts both the eggshell and embryo phenotypes to wild type indicating the insertion is responsible for the phenotype. Five excision lines have additional phenotypes: in one line wings of homozygous flies curve down and have extra veins and the remaining four are lethal.

(Rittenhouse and Berg, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

bwk⁰⁸⁴⁸²

(Andersen and Horne-Badovinac, 2016, Neuman-Silberberg, 2007)

bwk⁸⁴⁸²

(Rodríguez-Muñoz et al., 2022, Dorman et al., 2004, Tran and Berg, 2003, Roch et al., 2002, Jimenez et al., 2000)

cic⁰⁸⁴⁸²

fs(3)08482

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (15)

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