FlyBase Allele Report: Dmel\Ote[PK]

General Information

Symbol

Dmel\Ote^PK

Species

D. melanogaster

Name

FlyBase ID

FBal0031453

Feature type

allele

Associated gene

Dmel\Ote

Associated Insertion(s)

Carried in Construct

Also Known As

ote^halPK

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Barton et al., 2013)

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: R127term.

(Kitzman et al., 2022)

Amino acid replacement: ??term.

(Barton et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:18,276,701..18,276,701 [-]

Nucleotide change:

C18276701T

Amino acid change:

R127term | Ote-PA

Reported amino acid change:

R127term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Kitzman et al., 2022)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | adult stage (with Ote^B279)

(Barton et al., 2016)

abnormal developmental rate | female | larval stage (with Ote^B279)

(Barton et al., 2016)

abnormal size | third instar larval stage (with Ote^B279)

(Barton et al., 2016)

decreased cell number | adult stage | progressive (with Ote^B279)

(Barton et al., 2016)

decreased fecundity | female (with Ote^B279)

(Barton et al., 2016)

decreased fecundity | male | progressive (with Ote^B279)

(Barton et al., 2016)

decreased size | adult stage | female (with Ote^B279)

(Tsatskis et al., 2020)

female sterile

(Schupbach and Wieschaus, 1991)

female sterile (with Ote^B279)

(Tsatskis et al., 2020)

male semi-sterile | progressive (with Ote^B279)

(Tsatskis et al., 2020)

viable (with Ote^B279)

(Barton et al., 2014)

Phenotype Manifest In

escort cell | adult stage (with Ote^B279)

(Barton et al., 2016)

female germline cell (with Ote^B279)

(Barton et al., 2013)

female germline cell | adult stage (with Ote^B279)

(Barton et al., 2016)

female germline stem cell | adult stage (with Ote^B279)

(Barton et al., 2016)

fusome (with Ote^B279)

(Barton et al., 2013)

fusome (with Ote^EMS)

(Barton et al., 2013)

germarium (with Ote^B279)

(Barton et al., 2013)

germarium (with Ote^EMS)

(Barton et al., 2013)

germarium cap cell | adult stage | progressive (with Ote^B279)

(Barton et al., 2016)

germinal proliferation center hub | adult stage | progressive (with Ote^B279)

(Barton et al., 2016)

hub cell | adult stage (with Ote^B279)

(Barton et al., 2016)

male germline cell | adult stage (with Ote^B279)

(Barton et al., 2016)

male germline cell | third instar larval stage (with Ote^B279)

(Barton et al., 2016)

male germline stem cell | adult stage (with Ote^B279)

(Barton et al., 2016)

ovary | adult stage (with Ote^B279)

(Tsatskis et al., 2020)

spectrosome (with Ote^B279)

(Barton et al., 2013)

spectrosome (with Ote^EMS)

(Barton et al., 2013)

terminal filament | larval stage (with Ote^B279)

(Barton et al., 2016)

testis | third instar larval stage (with Ote^B279)

(Barton et al., 2016)

Detailed Description

Statement

Reference

Ote^B279/Ote^PK adults exhibit small ovaries.

(Tsatskis et al., 2020)

Ote^PK/Ote^B279 mutant females display a severe reduction in the number of eggs laid per day, and males are fertile in the first 6 days of adulthood but exhibit significantly decreased fertility with age. Ovaries from Ote^PK/Ote^B279 mutant adult females contain normal numbers of terminal filament cells, but decreased numbers of cap cells and escort cells, and some mutants show loss of germline stem cells, and absence of differentiating germ cells, as compared to wild type. Cap cell number further declines with age, and the decreased cap cell number is more pronounced in mutant germaria that do not contain germ cells. In the larval ovary, Ote^PK/Ote^B279 mutants display delayed terminal filament formation. A subset of Ote^PK/Ote^B279 mutant adult males display testis phenotypes, including varying degrees of germ cell and germline stem cell loss that worsen with age, decreased cell adhesion in the niche, on average fewer (but more variability in number of) hub cells in the niche, and increasing variability in hub size with age, but no defect in somatic cyst stem cell maintenance, as compared to wild type. There is no association of germ cell loss with canonical apoptosis. Male gonads are smaller in Ote^PK/Ote^B279 mutant third instar larvae than in wild type, and the niches in mutants have reduced germline stem cell numbers compared to wild type.

(Barton et al., 2016)

Ovaries from Ote^EMS/Ote^PK and Ote^B279/Ote^PK females have a range of mutant germarial phenotypes. These include germaria containing no germ cells, germaria containing fusomes only, germaria containing both spectrosomes and fusomes (the vast majority of this class carry a single developing cyst connected by an incompletely branched fusome) and germaria containing spectrosomes only (germaria in this class contain a higher number of spectrosome containing cells than normal). The "empty germaria" and "spectrosomes only" classes are recovered at high frequency.

Ovaries of Ote^B279/Ote^PK third instar larvae have a normal cellular organisation, with approximately 30% fewer primordial germ cells (PGCs) than age-matched control ovaries. The mutant PGCs all contain spectrosomes with no evidence of branching. Niches are formed in the mutant gonads at the pupal stage and occupied by germ cells, as occurs in wild type. However, the mutant pupal gonads do not contain differentiated egg chambers (egg chambers are established in wild type pupal gonads).

(Barton et al., 2013)

No egg chambers.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Nemp[+]/Nemp^RR1, Ote^PK/Ote^B279 has decreased cell number | female | adult stage phenotype

(Tsatskis et al., 2020)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has decreased occurrence of cell division | larval stage phenotype

(Barton et al., 2014)

MAN1^Δ26/MAN1^Δ81, Ote^PK/Ote^B279 has lethal phenotype

(Barton et al., 2014)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has some die during P-stage phenotype

(Barton et al., 2014)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has majority die during larval stage phenotype

(Barton et al., 2014)

MAN1^Δ26/MAN1^Δ81, Ote^PK/Ote^B279 has some die during P-stage phenotype

(Barton et al., 2014)

MAN1^Δ26/MAN1^Δ81, Ote^PK/Ote^B279 has some die during larval stage phenotype

(Barton et al., 2014)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has lethal phenotype

(Barton et al., 2014)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has abnormal mitotic cell cycle phenotype

(Barton et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Nemp[+]/Nemp^RR1, Ote^PK/Ote^B279 has ovary | adult stage phenotype

(Tsatskis et al., 2020)

Nemp[+]/Nemp^RR1, Ote^PK/Ote^B279 has female germline cell | adult stage | decreased number phenotype

(Tsatskis et al., 2020)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has larval brain | larval stage phenotype

(Barton et al., 2014)

Ote^PK/Ote^B279, bocks^Δ10/bocks^Δ66 has imaginal disc phenotype

(Barton et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Nemp^RR1/+; Ote^B279/Ote^PK ovaries exhibit a reduction in the number of Vasa[+] germ cells.

(Tsatskis et al., 2020)

Ote^B279/Ote^PK ; bocks^Δ10/bocks^Δ66 larval brains are small, with few proliferating cells, and imaginal discs are small or absent.

Ote^B279/Ote^PK ; MAN1^Δ81/MAN1^Δ26 larvae have normal sized brains and imaginal discs.

(Barton et al., 2014)

Germ cells with fusomes are found in ovaries one day after heat shock in Ote^PK/Ote^B279 females carrying bam^hs.PO. Three days after heat shock the mutant germaria are largely devoid of germ cells and the ovaries contain a very low number of egg chambers.

(Barton et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Ote^PK/Ote^B279 is partially rescued by Ote^nanos.PB

(Barton et al., 2016)

Not rescued by

Ote^PK/Ote^B279 is not rescued by Ote^UASp.cBa/Scer\GAL4^en.PU

(Barton et al., 2016)

Ote^PK/Ote^B279 is not rescued by Ote^UASp.cBa/Scer\GAL4^bab1-Gal4

(Barton et al., 2016)

Ote^PK/Ote^B279 is not rescued by Ote^UASp.cBa/Scer\GAL4^C587

(Barton et al., 2016)

Comments

Expression of Ote^nos.PB restores fertility, rescues the germline stem cell maintenance, differentiation germ cell number, somatic cell number and cell adhesion phenotypes of both Ote^PK/Ote^B279 mutant males and females, but does not fully restore hub size in mutant males.

Expression of Ote^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^en.PU, Scer\GAL4^bab1-Gal4, or Scer\GAL4^C587 fails to rescue the sterility, loss of germline stem cells, lack of differentiating germ cells, or loss of cap cells observed in Ote^PK/Ote^B279 mutant females.

(Barton et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605586

y¹ w^*; Ote^PK/CyO; P{UASp-Ote.B}51-3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Ote^PK

emerin^PK

(Kitzman et al., 2022)

hal^PK13

ote^PK

(Jones et al., 2024, Kitzman et al., 2022, Duan et al., 2020, Tsatskis et al., 2020, Barton et al., 2016, Barton et al., 2014, Barton et al., 2013)

ote^halPK

(Duan et al., 2020, Barton et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (8)

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