FlyBase Allele Report: Dmel\ft[k07918]

ft^k07918 mutants show planar cell polarity defects in the wings that extend distal to the posterior crossvein. The wings are foreshortened along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.

(Matakatsu and Blair, 2006)

When ft^k07918 somatic clones are made in the wing, clones contain significantly more cells than wild-type clones. These clones grow preferentially in the proximal part of the wing. When ft^k07918 mutant wing and eye imaginal discs are made with clones, an overgrowth phenotype is seen.

(Garoia et al., 2005)

The average number of crystal cells per embryo is reduced (to 16) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

(Milchanowski et al., 2004)

Homozygous ft^k07918 larvae show a small delay (1-2 days) to pupariation, and 60% of pupae reach pharate stages. Wing discs have a higher density

(1.2 times) than seen i wild-type. The pharate adults show abnormalities

in cuticular patterns and structures in all appendages. The adult

wing of pharates are about 1.5 time the size of wild-type, with a higher

cell number (2.9 times) and a higher trichome density (1.9 times).

The shape of the wing is broad and short, enlarged in the anterior

posterior axis compared with controls. The basal wing region is more

affected than distal regions, with vein abnormalities and abnormal

trichome polarity. The chaetae pattern of the wing margin and the

position of the vein sensillae are fairly normal, although chaetae

are blunter and thicker. Some apoptotic cells are seen in the wing

blade.

Somatic wing clones of homozygous ft^k07918 in a M(2)24F¹ background

tend to fill one or several intervein sectors but a fraction do cross

the dorsal ventral boundary. The clones tend to fill proximal regions

of the wing. The distal borders of clones are rounded, and are smooth

in contrast with the indented borders found in control clones. In

all of these features ft^k07918 and ft^G-rv are more extreme

than ft⁴. Clones can also cause outgrowths in the wing, generally

in the proximal part of the wing blade. There are blisters that evaginate

from the wing surface and have irregular trichome polarities, usually

perpendicular to the clone border. Trichome density in clones is higher

than wild-type clones - about 2.2 times that of wild-type, indicating

that cell size is smaller than wild-type. Mutant clones that overlap

veins differentiate thicker than normal vein ribbons. Chaetae within

clones are smaller and blunter than wild-type.

Somatic clones in the notum (of homozygous ft^k07918 in a M(2)24F¹

background) are large, with more cells than control clones, leading

to an increase in the total notum surface. They contain many more

chaetae (1.7 times wild-type) and higher cell density (1.4 times).

Similar deviations occur in the legs and head capsule. tergite clones

however show normal patterns of pigment chaetae and trichomes.

ft^k07918/ft^k07918 somatic clones in the wing, in a rho^ve-1/vn¹

background cover smaller territories than clones in wild-type. The

clones appear in central and proximal regions of the wing blade, but

distalise more than control ft^k07918 clones. Clones may

cover presumptive vein regions but never fail to differentiate vein

histotype.

ft^k07918/ft^k07918 somatic clones in the wing, in a Egfr^t1

background are smaller than clones in a wild-type background. ft^k07918/ft^k07918

somatic clones in the wing, in a Egfr background show more cells

than twin clones.

ft^k07918/ft^k07918 somatic clones in the wing, in a N^55e11/+

or a N^Ax-16/+ background do not depart from those in wild-type

wings.

wg^NZ/ft^k07918 somatic clones exhibit an additive phenotype

of the two alleles.

nub¹/ft^k07918 somatic clones are small and cause overgrowth,

as in ft^k07918 clones, but retain the extreme accommodation phenotype

of nub¹.

Su(H)^k07904/ft^k07918 somatic clones show additive phenotypes.

In ds^38k,ft^k07918 clones only epistatic effects of ft^k07918

are seen.

ft^k07918/ft⁴ and ft^k07918/ft^G-rv larvae show a delay

in pupariation of 1-2 days and have a maximal disc size similar to

that of ft^k07918 homozygotes.

ft^k07918,Su(H)^k07904 doubly mutant discs show a phenotype intermediate

between ft^k07918 and Su(H)^k07904 phenotypes.

(Garoia et al., 2000)

Mutants show lethal overgrowth of the brain and imaginal discs.

(Tick et al., 1999)

Mutants exhibit visible disc abnormalities: discs are overgrown. Clones induced in wild type discs are abnormal: clones are larger than controls. Clones in the wing cause extra veins and vein thickening.

(Roch et al., 1998)

Lethality occurs during pupal and pharate adult stages. Brain is occasionally larger than wild type. Leg discs develop extra folds, hyperplastic outgrowth.

(Torok et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ft^k07918 has increased cell number | somatic clone phenotype, enhanceable by rho^UAS.cdCa/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

ft^k07918 has increased cell number | somatic clone phenotype, enhanceable by Raf^UAS.F179/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

NOT Enhanced by

Statement

Reference

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-enhanceable by Raf^K497M.UAS/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-enhanceable by rho^UAS.cdCa/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-enhanceable by Raf^UAS.F179/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

Suppressed by

Statement

Reference

ft^k07918 has increased cell number | somatic clone phenotype, suppressible by Raf^K497M.UAS/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

NOT suppressed by

Statement

Reference

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-suppressible by Raf^K497M.UAS/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-suppressible by Raf^UAS.F179/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

ft^k07918 has abnormal planar polarity | somatic clone phenotype, non-suppressible by rho^UAS.cdCa/Scer\GAL4^Bx-MS1096

(Garoia et al., 2005)

Other

Statement

Reference

Myc^P0, ft^k07918 has abnormal cell growth | somatic clone phenotype

(Garoia et al., 2005)

ap^rK568, ft^k07918 has lethal | recessive phenotype

(Garoia et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

ft^k07918 has wing disc phenotype, enhanceable by nub²

(Garoia et al., 2000)

NOT Enhanced by

Statement

Reference

ft^k07918 has wing | somatic clone phenotype, non-enhanceable by tkv¹

(Garoia et al., 2005)

ft^k07918 has wing disc phenotype, non-enhanceable by Egfr^E3

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-enhanceable by rho^ve-1

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-enhanceable by rl^Sem

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-enhanceable by vn¹

(Garoia et al., 2000)

NOT suppressed by

Statement

Reference

ft^k07918 has wing | somatic clone phenotype, non-suppressible by tkv¹

(Garoia et al., 2005)

ft^k07918 has wing disc phenotype, non-suppressible by Egfr^E3

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-suppressible by rho^ve-1

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-suppressible by rl^Sem

(Garoia et al., 2000)

ft^k07918 has wing disc phenotype, non-suppressible by vn¹

(Garoia et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

When ft^k07918 somatic clones are made in the wing in a rho^{Scer\UAS.cdCa} or phl^{Scer\UAS.F179} background (driven by Scer\GAL4^Bx-MS1096) clones are several times larger than ft^k07918 clones in a wild-type background. The clones also show a homogeneous distribution in the wing. The polarity defects seen in ft^k07918 clones in a wild-type background are not affected. This enhancement of the ft^k07918 overgrowth phenotype is also seen when mutant eye and wing discs are made with clones. ft^k07918 clones are induced in a dm^P0 background at 72+-2h are severely reduced compared to those induced in wild-type. When ft^k07918 clones are induced in a tkv¹ background the size and allocation of clones is unaffected.

(Garoia et al., 2005)

ap^rK568, ft^k07918 double homozygotes are lethal (L1-2 phenoeffective phase). The double mutant combination nub², ft^k07918 causes a strong delay in pupation, allowing the wing discs to be considerably enlarged. These discs are larger than seen in ft^k07918 alone. The addition of Egfr^E3, Egfr^E3, vn¹ or rho^ve-1 has no effect on the ft^k07918 wing disc phenotype.

(Garoia et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ft^k07906

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10692

y¹ w^67c23; P{lacW}Glyp^k07918 ft^k07918/CyO

Kyoto

111230

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Glyp^k07918 ft^k07918 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

Separable from: P{lacW}GlyP^k07918. The overgrowth phenotype and lethality of ft^k07918 are due to a second site mutation on the "l(2)k07918" chromosome and are not associated with the P{lacW}GlyP^k07918 insertion.

(Tick et al., 1999)

Induced with: P{lacW}GlyP^k07918.

(Gelbart, 1999.7.20)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Lethality not caused by P{lacW}GlyP^k07918.

(Gelbart, 1999.7.20)

P-element excision demonstrates the insertion to be the cause of lethality.

(Torok et al., 1993)

Complements: l(2)k09624^k09624. Complements: l(2)k09932^k09932. Complements: l(2)k11021^k11021.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

79/18

(Roch et al., 1998)

P10692

(Milchanowski et al., 2004)

fat^k07918

(Gambis et al., 2011)

ft¹⁸

(Matakatsu and Blair, 2012, Matakatsu and Blair, 2006, Garoia et al., 2005, Garoia et al., 2000)

ft^k07918

(Matakatsu and Blair, 2006)

k07918

(Weber et al., 2012)

l(2)k07918^k07918

(Johannes and Preiss, 2002, Gelbart, 1999.7.20, Tick et al., 1999, BDGP Project Members, 1994-1999, Torok et al., 1993)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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