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General Information
Symbol
Dmel\Pka-C1E95
Species
D. melanogaster
Name
FlyBase ID
FBal0042698
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DC0E95, DCOE95, PKAE95
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Somatic clones of Pka-C1E95 homozygous cells in the posterior compartment of the adult wing blade persist after surrounding heterozygous or wild-type cells have died.

Homozygous clones in the notum induce formation of macrochaetae in adjacent wild-type cells. Only wild-type cells on or lateral to the dorsocentral (DC) meridian respond to the nearby presence of a homozygous clone by making extra macrochaetae. Most clones in the scutum lateral or medial to the DC meridian form pits, invaginations into the cuticle surface. In the medial prescutum, most homozygous clones form normal patterns containing only microchaetae and these clones do not contain macrochaetae.

Homozygous clones in the wing induce the formation of supernumerary wings. Within the supernumerary wing, the homozygous cells contribute to intervein tissue that normally forms posteriorly to vein 3.

Clones in the anterior compartment of the adult abdomen develop normally or almost normally when they arise near the anterior/posterior compartment boundary. Clones that arise more anteriorly in the a3 and a4 regions, form cuticle characteristic of a more posterior position (a5) within the anterior compartment, and the hairs and bristles produced are more crowded than in the surrounding wild-type tissue. This transformation is autonomous. The polarity of hairs and bristles both within and outside the clone is abnormal. Anterior clones are under-represented. Clones that are doubly mutant for Pka-C1E95 and wgl-17, or triply mutant for Pka-C1E95, wgl-17 and dppunspecified have the same phenotype as single mutant Pka-C1E95 clones. Clones that arise in the a2 anterior region of the anterior compartment are transformed to make naked a1 cuticle.

Homozygous mutant clones in the wing produce supernumerary double anterior wings and in the leg anterior clones both ventrally and dorsally cause supernumerary double anterior hindlegs.

Ommatidia within a clone are invariably mutant, ommatidia may have extra or less photoreceptor cells, appear to be of either chiral form and in a random orientation. Ommatidia in a region posterior to a clone are of the inappropriate chiral form but they are still mirror reflection either side of the equator. This presumable reflects the backward flow of the ectopic wave before it meets the anteriorly advancing endogenous wave. Ommatidia anterior to the clones have the correct chirality and orientation as the morphogenetic ectopic wave is moving in the correct posterior to anterior direction. It is unclear of ommatidial chirality is perturbed in regions where the morphogenetic wave flows perpendicular to the normal direction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Pka-C1E95 has visible | recessive phenotype, suppressible by dppH61

NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Pka-C1E95 has adult cuticle & abdomen | somatic clone phenotype, non-suppressible by Df(2L)RF

Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

When Pka-C1E95, Df(2L)RF (which removes the genes Wnt4, Wnt6 and Wnt10) homozygous clones are made in the adult abdomen, the polarity is reversed in the same way as Pka-C1E95 clones are.

Pka-C1E95, sggM11 smo3 triple mutant clones in the wing disc exhibit some outgrowths from the notum but no significant wing pattern duplications. Wing discs with Pka-C1E95, smo3 clones have large expansions affecting the anterior compartment of the wing disc.

The non-autonomous extra macrochaetae phenotype of homozygous clones is suppressed if the clones are also homozygous for dppH61. Unlike Pka-C1E95 clones, sgg32 Pka-C1E95 double mutant clones in the medial prescutum produce macrochaetae autonomously, and unlike sgg32 clones, the double mutant clones have a symmetrical bristle pattern. In the posterior scutum, sgg32 Pka-C1E95 double mutant clones have a scutellar phenotype very similar to that of Pka-C1E95 clones. In the anterior scutum, sgg32 Pka-C1E95 double mutant clones no longer show the non-autonomous induction of macrochaetae or formation of pits characteristic of Pka-C1E95 clones. Formation of macrochaetae within the double mutant clones is increased relative to sgg32 clones.

Clones double mutant for dppH61 Pka-C1E95 do not cause supernumerary structures in the leg and wing. Clones positioned at the compartment boundary exhibit long range effects causing a marked reduction in wing size and loss of vein pattern in anterior and posterior compartments. Short range effects include inducing immediate neighbours to form ectopic veins and margin bristles, the clones fail to differentiate wing veins in a cell autonomous fashion. dppd12 Pka-C1E95 also fail to form veins in the anterior compartment. wgl-17 Pka-C1E95 clones cause local outgrowths of leg tissue and on rare occasions form of supernumerary legs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Global rearrangements of the wing and leg occur as the clones express dpp.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (16)