FlyBase Allele Report: Dmel\dsh[UAS.cAa]

General Information

Symbol

Dmel\dsh^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Axelrod

FlyBase ID

FBal0049443

Feature type

allele

Associated gene

Dmel\dsh

Associated Insertion(s)

Carried in Construct

P{UAS-dsh.A}

Also Known As

UAS-dsh, UAS.Dsh, UASdsh

Key Links

Transgenic product class

cDNA

(Axelrod et al., 1996)

wild_type

(Axelrod et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Axelrod et al., 1996)

wild_type

(Axelrod et al., 1996)

Mutagen

in vitro construct

(Axelrod et al., 1998, Axelrod et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-dsh.A}

Cytology

Description

Expression of dsh cDNA is governed by UAS regulatory sequences.

(Axelrod et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR.PF

(Sato et al., 2006)

lethal | heat sensitive, with Scer\GAL4^ptc-559.1

(Lee and Adler, 2002)

visible, with Scer\GAL4^GMR.PF

(Waldrop et al., 2006, Rousset et al., 2001)

visible, with Scer\GAL4^ptc-559.1

(Axelrod et al., 1996)

visible, with Scer\GAL4^rho-rhl

(Axelrod et al., 1996)

visible, with Scer\GAL4^Ubx.PdC

(Phillips et al., 1999)

Phenotype Manifest In

embryonic/first instar larval cuticle, with Scer\GAL4^prd.RG1

(Metcalfe et al., 2010)

embryonic/first instar larval cuticle, with Scer\GAL4^{VP16.mat.αTub67C}

(Sun et al., 2001, Axelrod et al., 1998)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Chung et al., 2009)

eye, with Scer\GAL4^GMR.PF

(Waldrop et al., 2006, Rousset et al., 2001)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Sato et al., 2006)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Rousset et al., 2001)

lamina, with Scer\GAL4^GMR.PF

(Sato et al., 2006)

macrochaeta, with Scer\GAL4^ptc-559.1

(Lee and Adler, 2002)

macrochaeta, with Scer\GAL4^Ubx.PdC

(Phillips et al., 1999)

macrochaeta & scutellum, with Scer\GAL4^ptc-559.1

(Axelrod et al., 1996)

microchaeta & scutellum, with Scer\GAL4^ptc-559.1

(Axelrod et al., 1996)

microchaeta & scutum, with Scer\GAL4^rho-rhl

(Axelrod et al., 1996)

neuron, with Scer\GAL4^GMR.PF

(Sato et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Rousset et al., 2001)

sensory mother cell & dorsal mesothoracic disc | ectopic, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

stalk follicle cell | increased number | heat sensitive, with Scer\GAL4^hs.PB

(Song and Xie, 2003)

ventral denticle belt, with Scer\GAL4^prd.RG1

(Metcalfe et al., 2010)

ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Sun et al., 2001)

wing hair & 1st posterior cell, with Scer\GAL4^ptc-559.1

(Lee and Adler, 2002)

Detailed Description

Statement

Reference

Overexpression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^prd.RG1 in embryos produces clear expansions of the naked cuticle zones that are interspersed between the ventral denticle belts of wild-type embryos.

(Metcalfe et al., 2010)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^btl.PS results in dorsal trunks that are shorter than normal and are discontinuous.

(Chung et al., 2009)

Expression of dsh^Scer\UAS.cAa in the retina behind the furrow using Scer\GAL4^GMR.PF results in the projection of ventral R axons into the dorsal lamina.

(Sato et al., 2006)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^hs.PB using four days of pulsed heat shock treatment consistently produces more follicle cells that accumulate between egg chambers. These extra follicle cells form long stalks with multiple rows of cells instead of one row in the wild type.

(Song and Xie, 2003)

When expression is driven by Scer\GAL4^ptc-559.1 at 18^oC adults often eclose and a multiple wing hair phenotype results. In addition, ecoptic bristles are found.

(Lee and Adler, 2002)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^dpp.blk1 inhibits furrow initiation in the eye disc.

(Kumar and Moses, 2001)

dsh^Scer\UAS.cAa when driven by Scer\GAL4^GMR.PF produces small bristle-less eyes devoid of ommatidia.

(Rousset et al., 2001)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in loss of denticles and expansion of smooth cuticle.

(Sun et al., 2001)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^da.G32 results in a phenotype identical to that produced by overexpression of wg.

(Buratovich et al., 2000)

Expression of dsh^Scer\UAS.cAa driven by Scer\GAL4^109(2)80 does not give rise to any detectable dendritic phenotypes.

(Gao et al., 2000)

When expression of dsh^Scer\UAS.cAa is driven by Scer\GAL4^sd-SG29.1, the whole of the anterior compartment of the wing disc is covered in evenly spaced SOPs (sensory organ precursors).

(Brennan et al., 1999)

Clones in the notum expressing dsh^Scer\UAS.cAa under the control of Scer\GAL4^Ubx.PdC develop extra macrochaetae.

(Phillips et al., 1999)

Embryos expressing dsh^Scer\UAS.cAa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} have naked cuticle.

(Axelrod et al., 1998)

When driven by Scer\GAL4^ptc-559.1 duplication of the wg-dependent scutellar bristles occurs. Scer\GAL4^rho-rhl-mediated expression causes an increase in the density of macrochaetae on the notum.

(Axelrod et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, dsh^UAS.cAa has visible phenotype, suppressible by Scer\GAL4^GMR.PF/nkd^UAS.cZa

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has visible phenotype, suppressible | partially by nkd^{ΔR1S.UAS.EGFP}/Scer\GAL4^GMR.PF

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has visible phenotype, suppressible by nkd^{R1S.UAS.Tag:MYC}/Scer\GAL4^GMR.PF

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has visible phenotype, suppressible by nkd^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Rousset et al., 2001)

Other

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has visible phenotype

(Quijano et al., 2011)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has abnormal planar polarity phenotype

(Lee and Adler, 2002)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, in^IH56 has abnormal planar polarity phenotype

(Lee and Adler, 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, dsh^UAS.cAa has eye phenotype, suppressible by Scer\GAL4^GMR.PF/nkd^UAS.cZa

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has eye phenotype, suppressible | partially by nkd^{ΔR1S.UAS.EGFP}/Scer\GAL4^GMR.PF

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has eye phenotype, suppressible by nkd^{R1S.UAS.Tag:MYC}/Scer\GAL4^GMR.PF

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has interommatidial bristle phenotype, suppressible by nkd^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Rousset et al., 2001)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has eye phenotype, suppressible by nkd^UAS.Tag:MYC/Scer\GAL4^GMR.PF

(Rousset et al., 2001)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, dsh^UAS.cAa has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/fz2^{ECD.GPI.UAS.Tag:MYC}

(Rousset et al., 2001)

Scer\GAL4^GMR.PF, dsh^UAS.cAa has interommatidial bristle phenotype, non-suppressible by Scer\GAL4^GMR.PF/fz2^{ECD.GPI.UAS.Tag:MYC}

(Rousset et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^prd.RG1/dsh^UAS.cAa is a suppressor of ventral denticle belt | maternal effect phenotype of arr²

(Metcalfe et al., 2010)

Scer\GAL4^prd.RG1/dsh^UAS.cAa is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of arr²

(Metcalfe et al., 2010)

dsh^UAS.cAa/Scer\GAL4^da.G32 is a suppressor of denticle | ectopic phenotype of wg^l-17

(Morel and Arias, 2004)

dsh^UAS.cAa/Scer\GAL4^da.G32 is a suppressor of embryonic leading edge cell primordium phenotype of wg^l-17

(Morel and Arias, 2004)

dsh^UAS.cAa/Scer\GAL4^da.G32 is a suppressor of embryonic/first instar larval cuticle phenotype of wg^l-17

(Morel and Arias, 2004)

dsh^UAS.cAa, Scer\GAL4^arm.PS is a suppressor | partially of embryonic/first instar larval cuticle phenotype of Axn^UAS.GFP, Scer\GAL4^arm.PS

(Cliffe et al., 2003)

dsh^UAS.cAa/Scer\GAL4^69B is a suppressor of amnioserosa phenotype of hep¹

(Kaltschmidt et al., 2002)

dsh^UAS.cAa/Scer\GAL4^69B is a suppressor of embryonic dorsal epidermis phenotype of hep¹

(Kaltschmidt et al., 2002)

dsh^UAS.cAa/Scer\GAL4^69B is a suppressor of embryonic leading edge cell & actin filament phenotype of hep¹

(Kaltschmidt et al., 2002)

dsh^UAS.cAa/Scer\GAL4^69B is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of hep¹

(Kaltschmidt et al., 2002)

Scer\GAL4^prd.RG1/dsh^UAS.cAa is a suppressor of denticle phenotype of arr^unspecified

(Wehrli et al., 2000)

Scer\GAL4^prd.RG1/dsh^UAS.cAa is a suppressor of embryonic epidermis phenotype of arr^unspecified

(Wehrli et al., 2000)

dsh^UAS.cAa is a suppressor of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Other

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing sensillum phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing margin bristle | ectopic phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing vein L3 | distal phenotype

(Quijano et al., 2011)

Scer\GAL4^prd.RG1, dsh^UAS.cAa, wg^l-17 has embryonic/first instar larval cuticle | maternal effect phenotype

(Metcalfe et al., 2010)

Scer\GAL4^prd.RG1, dsh^UAS.cAa, wg^l-17 has ventral denticle belt | maternal effect phenotype

(Metcalfe et al., 2010)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has macrochaeta phenotype

(Lee and Adler, 2002)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, fy² has wing hair & 1st posterior cell phenotype

(Lee and Adler, 2002)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, in^IH56 has macrochaeta phenotype

(Lee and Adler, 2002)

Scer\GAL4^ptc-559.1, dsh^UAS.cAa, in^IH56 has wing hair & 1st posterior cell phenotype

(Lee and Adler, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dsh^Scer\UAS.cAa and Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 results in wings with no L1 or L3 sensilla, ectopic mechanosensory bristles on the dorsal margin and expansion of the L3 vein distally.

(Quijano et al., 2011)

None of the embryos that are mutant for both maternal and zygotic arr² and express Scer\GAL4^prd.RG1>dsh^Scer\UAS.cAa exhibit broad stripes of excess cuticle.

Scer\GAL4^prd.RG1>dsh^Scer\UAS.cAa-overexpressing embryos are readily identifiable amongst wg^l-17 homozygotes by their four wide naked zones interspersed between their denticle bands.

(Metcalfe et al., 2010)

Co-expression of nkd^{ΔR1S.Scer\UAS.T:Avic\GFP-EGFP} only weakly rescues the eye phenotype caused by expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF.

(Waldrop et al., 2006)

dsh^Scer\UAS.cAa; Scer\GAL4^da.G32 partially suppresses the transformation of naked ventral cuticle to denticles and the shortening of the cuticle seen in wg^l-17 homozygous embryos. The dorsal side of these cuticles have some 'warts'. dsh^Scer\UAS.cAa; Scer\GAL4^da.G32 suppresses the loss of dorsal ventral polarity in the leading edge cells of stage 13 wg^l-17 homozygous embryos, but only weakly rescues elongation of these cells.

(Morel and Arias, 2004)

The addition of dsh^Scer\UAS.cAa partially restores areas of naked cuticle to Axn^Scer\UAS.cHa, Scer\GAL4^arm.PS mutants. Also an abundance of small denticles are seen.

(Cliffe et al., 2003)

The dorsal hole phenotype, loss of leading edge actin cable, and loss of adhesion between the amnioserosa and dorsal epidermis of hep¹ mutant embryos are all partially suppressed by Scer\GAL4^69B with dsh^Scer\UAS.cAa.

(Kaltschmidt et al., 2002)

The multiple hair cell phenotype of dsh^Scer\UAS.cAa expressed from Scer\GAL4^ptc-559.1 is additive with the multiple hair phenotypes of fy² and in^IH56.

(Lee and Adler, 2002)

nkd^{Scer\UAS.T:Hsap\MYC} strongly suppresses the dsh^Scer\UAS.cAa, Scer\GAL4^GMR.PF phenotype restoring numerous bristles and ommatidia.

The addition of fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} to dsh^Scer\UAS.cAa, Scer\GAL4^GMR.PF results in some eye necrosis, but has a negligible effect on eye phenotype caused by dsh^Scer\UAS.cAa and Scer\GAL4^GMR.PF alone.

nkd^Scer\UAS.cZa strongly suppresses the dsh^Scer\UAS.cAa, Scer\GAL4^GMR.PF phenotype restoring numerous bristles and ommatidia. The addition of fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} to dsh^Scer\UAS.cAa, Scer\GAL4^GMR.PF results in some eye necrosis, but has a negligible effect on eye phenotype caused by dsh^Scer\UAS.cAa and Scer\GAL4^GMR.PF alone.

(Rousset et al., 2001)

Expression of dsh^Scer\UAS.cAa under the control of Scer\GAL4^{arm.T:Hsim\VP16} in an arm³ background gives rise to a cuticle with many small denticles, but with the length of an arm³ single mutant.

(Cox et al., 2000)

Smooth cuticle is restored in alternate segments to arr^unspecified embryos lacking maternal and zygotic arr function when dsh^Scer\UAS.cAa is expressed in these embryos under the control of Scer\GAL4^prd.RG1.

(Wehrli et al., 2000)

Strongly suppresses the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dsh^UAS.cAa/Scer\GAL4^da.G32 rescues dsh³

(Morel and Arias, 2004)

Comments

dsh^Scer\UAS.cAa; Scer\GAL4^da.G32 rescues the cuticles of embryos from dsh³ germline clones to normal length and suppresses the hole anterodorsal cuticle hole.

(Morel and Arias, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Used to show that over-expression of dsh (hence an increase in wg signalling) in the posterior wing compartment inhibits wing margin N activity.

(Rulifson et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dsh^Scer\UAS.cAa

dsh^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Axelrod

(Axelrod et al., 1996)

Secondary FlyBase IDs

References (30)

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