FlyBase Allele Report: Dmel\tkv[Q253D.UAS.cLa]

General Information

Symbol

Dmel\tkv^{Q253D.UAS.cLa}

Species

D. melanogaster

Name

FlyBase ID

FBal0051062

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.Q253D.L}

Also Known As

UAS-tkv*, UAS-tkv^QD

Key Links

Transgenic product class

missense_variant

(Deshpande et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Deshpande et al., 2014)

Mutagen

in vitro construct

(de Celis, 1997, Lecuit et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-tkv.Q253D.L}

Cytology

Description

Amino acid replacement: Q253D.

(Deshpande et al., 2014)

UAS regulatory sequences drive expression of a constitutively active form of tkv.

(Lecuit et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^Ser.PGF

(Kim et al., 2006)

some die during pupal stage | heat sensitive, with Scer\GAL4^Ser.PGF

(Kim et al., 2006)

visible, with Scer\GAL4^GMR.PF

(Bach et al., 2003)

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^CY2

(Zartman et al., 2008)

dorsal tracheal branch primordium, with Scer\GAL4^btl.PS

(Dorfman et al., 2002)

egg operculum, with Scer\GAL4^CY2

(Zartman et al., 2008)

embryonic/first instar larval cuticle, with Scer\GAL4^69B

(Fernandez et al., 2000)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^69B

(Llimargas and Casanova, 1997)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Llimargas and Casanova, 1997, Wappner et al., 1997)

embryonic/larval tracheal lateral trunk, with Scer\GAL4^69B

(Llimargas and Casanova, 1997)

embryonic/larval visceral tracheal branch, with Scer\GAL4^btl.PS

(Wappner et al., 1997)

eye, with Scer\GAL4^GMR.PF

(Bach et al., 2003)

germline cell, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

gonad, with Scer\GAL4^{VP16.nanos.UTR}

(Deshpande et al., 2014)

larval muscle system, with Scer\GAL4^btl.PS

(Franch-Marro and Casanova, 2000)

tracheal pit, with Scer\GAL4^btl.PS

(Franch-Marro and Casanova, 2000)

tracheocyte, with Scer\GAL4^69B

(Llimargas and Casanova, 1997)

tracheocyte, with Scer\GAL4^btl.PS

(Llimargas and Casanova, 1997)

wing, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

wing, with Scer\GAL4^bs-1348

(de Celis, 1997)

wing, with Scer\GAL4^C-580

(de Celis, 1997)

wing, with Scer\GAL4^Ser.PGF

(Kim et al., 2006)

wing vein | ectopic, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

wing vein | ectopic, with Scer\GAL4^bs-1348

(de Celis, 1997)

wing vein | ectopic, with Scer\GAL4^C-580

(de Celis, 1997)

Detailed Description

Statement

Reference

Expression of tkv^{Q253D.Scer\UAS.cLa} in the germline, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, does not generate any abnormalities in somatic development. There are little if any defects in germ cell migration and most primordial germ cells are properly aligned with the somatic germ cell precursors in stage 13 embryos. These mutants exhibit a substantial reduction in the number of germ cells on the coalesced gonad, apparently due to apoptosis.

(Deshpande et al., 2014)

Expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^CY2 results in eggshells that lack dorsal appendages and have an operculum that is increased in size.

(Zartman et al., 2008)

tkv^{Q253D.Scer\UAS.cLa}; Scer\GAL4^Ser.PGF animals raised at 25^oC die as pupae. Those raised at 18^oC survive to adulthood but have deformed, wrinkled wings.

(Kim et al., 2006)

Flies expressing tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^GMR.PF have rough, glassy eyes.

(Bach et al., 2003)

The number of cells in the lateral trunk anterior branch is not altered by expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^btl.PS. There is an excess of dorsal branch cells in these embryos.

(Dorfman et al., 2002)

When tkv^{Q253D.Scer\UAS.cLa} is driven by Scer\GAL4⁶⁰⁴ extravein tissue surrounds wing vein L5, which severely distorts and thickens the wing.

(Morey et al., 2001)

Embryos expressing tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^69B show partial dorsalisation of their cuticles.

(Fernandez et al., 2000)

When tkv^{Q253D.Scer\UAS.cLa} is driven by Scer\GAL4^btl.PS tracheal cells are induced to migrate in the dorsal ventral axis. The gap between the most dorsal muscle precursors and the more ventral ones are missing.

(Franch-Marro and Casanova, 2000)

When expressed via Scer\GAL4^69B, tracheal tree is modified such that only dorsoventral tracheal branches arise while longitudinal branches fail to form. When expressed via Scer\GAL4^btl.PS, causes failure of the tracheal cells to migrate anteroposteriorly to form the dorsal trunk. The visceral branches and the lateral trunk usually form.

(Llimargas and Casanova, 1997)

Scer\GAL4^btl.PS-mediated expression diverts cells of the dorsal trunk and visceral branch primordia to the dorsal branch.

(Wappner et al., 1997)

Produces ectopic vein tissue in the wing when expressed using Scer\GAL4^C-580 or Scer\GAL4^bs-1348.

(de Celis, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-enhanceable by Stat92E^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-enhanceable by dome^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-enhanceable by hop^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-enhanceable by upd1^unspecified

(Bach et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, suppressible by gl^unspecified

(Bach et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-suppressible by Stat92E^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-suppressible by dome^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-suppressible by hop^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has visible phenotype, non-suppressible by upd1^unspecified

(Bach et al., 2003)

Suppressor of

Statement

Reference

tkv^{Q253D.UAS.cLa}/Scer\GAL4^ey.PH is a suppressor | partially of visible phenotype of upd1^unspecified/Df(1)os1A2

(Bach et al., 2003)

tkv^{Q253D.UAS.cLa}/Scer\GAL4^btl.PS is a suppressor | partially of abnormal size phenotype of Scer\GAL4^btl.PS, arm^{S10.UAS.Tag:MYC}

(Llimargas, 2000)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-enhanceable by Stat92E^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-enhanceable by dome^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-enhanceable by hop^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-enhanceable by upd1^unspecified

(Bach et al., 2003)

Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, non-enhanceable by Sps1^k11320

(Morey et al., 2001)

Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa} has wing phenotype, non-enhanceable by Sps1^k11320

(Morey et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, tkv^{Q253D.UAS.cLa} has wing phenotype, suppressible by Tollo^UAS.cKa

(Kim et al., 2006)

Scer\GAL4^GMR.PU, tkv^{Q253D.UAS.cLa} has eye phenotype, suppressible by Tollo^UAS.cKa/Scer\GAL4^GMR.PU

(Kim et al., 2006)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, suppressible by gl^unspecified

(Bach et al., 2003)

Scer\GAL4^btl.PS, tkv^{Q253D.UAS.cLa}, vvl^GA3 has tracheocyte phenotype, suppressible by btl^hs.PM

(Llimargas and Casanova, 1997)

Scer\GAL4^btl.PS, tkv^{Q253D.UAS.cLa}, vvl^GA3 has larval tracheal system phenotype, suppressible by btl^hs.PM

(Llimargas and Casanova, 1997)

Scer\GAL4^C-580, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, suppressible by E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^C-580

(de Celis, 1997)

Scer\GAL4^bs-1348, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, suppressible by E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^bs-1348

(de Celis, 1997)

Scer\GAL4^C-580, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, suppressible by Raf^DN.UAS, Scer\GAL4^C-580

(de Celis, 1997)

Scer\GAL4^bs-1348, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, suppressible by Raf^DN.UAS, Scer\GAL4^bs-1348

(de Celis, 1997)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-suppressible by upd1^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-suppressible by Stat92E^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-suppressible by dome^unspecified

(Bach et al., 2003)

Scer\GAL4^GMR.PF, tkv^{Q253D.UAS.cLa} has eye phenotype, non-suppressible by hop^unspecified

(Bach et al., 2003)

Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa} has wing phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa} has wing vein | ectopic phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

Scer\GAL4^69B, tkv^{Q253D.UAS.cLa} has embryonic/first instar larval cuticle phenotype, non-suppressible | germline clone by Shark¹

(Fernandez et al., 2000)

Suppressor of

Statement

Reference

tkv^{Q253D.UAS.cLa} is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, Tollo^UAS.cKa

(Kim et al., 2006)

tkv^{Q253D.UAS.cLa} is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, Tollo^ex.UAS

(Kim et al., 2006)

tkv^{Q253D.UAS.cLa}/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Tollo^UAS.cKa

(Kim et al., 2006)

tkv^{Q253D.UAS.cLa}/Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of upd1^unspecified/Df(1)os1A2

(Bach et al., 2003)

tkv^{Q253D.UAS.cLa}/Scer\GAL4^btl.PS is a suppressor | partially of embryonic/larval tracheal dorsal trunk phenotype of Scer\GAL4^btl.PS, arm^{S10.UAS.Tag:MYC}

(Llimargas, 2000)

NOT Suppressor of

Statement

Reference

tkv^{Q253D.UAS.cLa}/Scer\GAL4^69B is a non-suppressor of embryo | germline clone | dorsal closure stage phenotype of Shark¹

(Fernandez et al., 2000)

Other

Statement

Reference

Scer\GAL4^btl.PS, elB^EP2039, tkv^{Q253D.UAS.cLa} has tracheal lateral trunk anterior branch primordium phenotype

(Dorfman et al., 2002)

Scer\GAL4^btl.PS, tkv^{Q253D.UAS.cLa}, vvl^GA3 has larval tracheal system phenotype

(Llimargas and Casanova, 1997)

Scer\GAL4^btl.PS, tkv^{Q253D.UAS.cLa}, vvl^GA3 has tracheocyte phenotype

(Llimargas and Casanova, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The deformed, wrinkled wing phenotype seen in tkv^{Q253D.Scer\UAS.cLa}; Scer\GAL4^Ser.PGF flies is completely suppresessed by Tollo^Scer\UAS.cKa or Tollo^ex.Scer\UAS.

(Kim et al., 2006)

The small eye phenotype of upd1^unspecified/Df(1)os1A2 flies is partly suppressed by expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^ey.PH. The rough eye phenotype caused by expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^GMR.PF is strongly suppressed by gl^unspecified. The eye phenotype caused by expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^GMR.PF is partially modified by Mad^unspecified.

(Bach et al., 2003)

Co-expression of both elB^EP2039 and tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^btl.PS results in an excess of lateral trunk anterior branch cells in the embryo.

(Dorfman et al., 2002)

The cuticle phenotype of embryos expressing tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^69B is not altered if they are derived from homozygous shark¹ female germ-line clones (and fertilised by a shark⁺ sperm). The dorsal closure defects of homozygous shark¹ embryos derived from shark¹ female germline clones are not rescued by expression of tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^69B.

(Fernandez et al., 2000)

When tkv^{Q253D.Scer\UAS.cLa} and arm^{S10.Scer\UAS.T:Hsap\MYC} are co-expressed using Scer\GAL4^btl.PS, a partial restoration of the dorsal trunk is seen in the tracheal system.

(Llimargas, 2000)

When expressed via Scer\GAL4^btl.PS in a vvl^GA3 mutant background, no migration of tracheal cells occurs unless btl is also expressed, via btl^hs.PM.

(Llimargas and Casanova, 1997)

The ectopic vein phenotype seen when tkv^{Q253D.Scer\UAS.cLa} expressed using Scer\GAL4^C-580 or Scer\GAL4^bs-1348 is reduced in combination with HLHmβ^{Scer\UAS.cdCa} or phl^DN.Scer\UAS.

(de Celis, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Tkv^*

(Wu and Cohen, 1999)

UAS-tkv^*

tkv^{Q253D.Scer\UAS.cLa}

tkv^{Q253D.UAS.cLa}

Name Synonyms

Secondary FlyBase IDs

References (26)

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