FlyBase Allele Report: Dmel\dco[S091510]

General Information

Symbol

Dmel\dco^S091510

Species

D. melanogaster

Name

FlyBase ID

FBal0100078

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

P{lacW}dco^S091510

Carried in Construct

Also Known As

dco^P915

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zilian et al., 1999)

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}dco^S091510

Cytology

Description

Insertion of P{lacW} into the second (5' UTR) intron of the dco transcription unit, 766bp downstream of its 5' end.

(Zilian et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:31,059,608..31,059,608 [-]

(Zilian et al., 1999, Szeged Stock Center, 1998-, Deak et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Zilian et al., 1999, Szeged Stock Center, 1998-)

lethal - all die before end of pupal stage | recessive

(Deak et al., 1997)

some die during pupal stage | recessive

(Deak et al., 1997)

Phenotype Manifest In

adult external head

(Zilian et al., 1999)

imaginal disc

(Zilian et al., 1999)

tarsal segment

(Zilian et al., 1999)

Detailed Description

Statement

Reference

dco^S091510/dco^S139602 hypomorphs are developmentally delayed. They exhibit a dramatic increase in apoptosis in the wing discs compared to wild-type. Elevated apoptosis (assessed through the use of TUNEL) is also found in the leg and haltere discs, while no increase is detected in the eye imaginal discs.

(Guan et al., 2007)

Homozygotes show lethality during larval or early pupal stages, abnormal discs with granular zone and abnormal folding. dco³ in combination with dco^le88, dco^i3-193, dco^S053813, dco^S139602, dco^S091510 and dco^P103 : show lethality during larval or early pupal stages with typical dco³ phenotype (swollen tarsi with or without head defects). Discs overgrown. dco¹⁸ in combination with dco^S139602 or dco^S091510 : show lethality during larval or early pupal stages with a discless phenotype. dco^le88 in combination with dco^S091510 : show lethality during larval or early pupal stages with a small discs phenotype. dco^i3-193 in combination with dco^S091510 : show lethality during larval or early pupal stages with small discs phenotype. dco^S053813 in combination with dco^S091510 : show lethality during larval or early pupal stages with a small disc phenotype. dco^S139602 in combination with dco^S091510 : show lethality during larval or early pupal stages, normal discs. dco^S091510 in combination with dco^P103 : adult viable with normal phenotype, normal discs. dco^S091510 in combination with dco^S144701 : adult viable with normal phenotype.

(Zilian et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Levels of apoptosis in dco^S091510/dco^S139602 hypomorphs are reduced in a Df(3L)H99 background. This is correlated with marked acceleration in disc growth and larval development (from 8-10 days to 5-6 days). These Df(3L)H99/+, dco^S091510/dco^S139602 animals have increased survival into late pupal stages, and the veination pattern of the majority of these animals is simlar to wild-type. Occasional escapers survive to adulthood and display normal legs and wrinkled or blistered wings. These results suggest that the developmental delay in disc growth and larval development seen in dco^S091510/dco^S139602 mutants is largely caused by elevated apoptosis.