FlyBase Allele Report: Dmel\trio[S036810]

General Information

Symbol

Dmel\trio^S036810

Species

D. melanogaster

Name

FlyBase ID

FBal0100112

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

P{lacW}trio^S036810

Carried in Construct

Also Known As

l(3)s036810, trio^P0368/10, 0368/10

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Awasaki et al., 2000, Liebl et al., 2000, Newsome et al., 2000)

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}trio^S036810

Cytology

Description

Insertion of P{lacW} in the intron that separates the coding sequence of glycine at amino acid 1756. P{lacW} is orientated such that the direction of transcription of the Ecol\lacZ is the same as that of trio.

(Awasaki et al., 2000)

Insertion of P{lacW} into an intron in the coding region of the P{lacW} transcription unit, between coding exons 7 and 8.

(Liebl et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:1,003,654..1,009,321 [-]

(Awasaki et al., 2000, Liebl et al., 2000, Newsome et al., 2000, Szeged Stock Center, 1998-, Deak et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Df(3L)Fpa1)

(Awasaki et al., 2000)

lethal | recessive

(Szeged Stock Center, 1998-)

partially lethal - majority die (with Df(3L)Fpa1)

(Awasaki et al., 2000)

partially lethal - majority die | recessive

(Deak et al., 1997)

partially lethal - majority live (with trio^M89)

(Dean et al., 2013)

some die during P-stage (with trio^M89)

(Dean et al., 2013)

sterile

(Awasaki et al., 2000)

viable

(Awasaki et al., 2000)

viable (with trio^M89)

(Liebl et al., 2000)

visible (with trio^M89)

(Liebl et al., 2000)

Phenotype Manifest In

adult mushroom body (with Df(3L)Fpa1)

(Awasaki et al., 2000)

adult mushroom body (with trio^E4.1)

(Awasaki et al., 2000)

ellipsoid body (with Df(3L)Fpa1)

(Awasaki et al., 2000)

ellipsoid body (with trio^E4.1)

(Awasaki et al., 2000)

mushroom body medial lobe (with Df(3L)Fpa1)

(Awasaki et al., 2000)

mushroom body medial lobe (with trio^E4.1)

(Awasaki et al., 2000)

mushroom body vertical lobe (with Df(3L)Fpa1)

(Awasaki et al., 2000)

mushroom body vertical lobe (with trio^E4.1)

(Awasaki et al., 2000)

wing (with Df(3L)Fpa1)

(Awasaki et al., 2000)

wing (with trio^M89)

(Liebl et al., 2000)

Detailed Description

Statement

Reference

Shows reduced locomotor activity and failure of wing extension when heterozygous with Df(3L)Fpa1. In both trio^S036810/trio^E4.1 and trio^S036810/Df(3L)Fpa1 the LV and LM lobes of the mushroom bodies are abnormally developed. In the adult mushroom bodies the α, β abd γ lobes are altered. The ellipsoid body is also affected, with variable severity. The axons extend to the correct region but fail to form their arborization pattern.

(Awasaki et al., 2000)

Heterozygotes of trio^S036810/trio^M89 show a wing blister phenotype but are otherwise wild type.

(Liebl et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

trio^M89/trio^S036810 has partially lethal - majority live phenotype, enhanceable by seq^9.17/seq[+]

(Dean et al., 2013)

trio^M89/trio^S036810 has some die during P-stage phenotype, enhanceable by seq^9.17/seq[+]

(Dean et al., 2013)

trio^M89/trio^S036810 has lethal phenotype, enhanceable by Abl¹/Abl[+]

(Liebl et al., 2000)

trio^M89/trio^S036810 has lethal phenotype, enhanceable by Abl⁴/Abl[+]

(Liebl et al., 2000)

trio^M89/trio^S036810 has lethal phenotype, enhanceable by fax^M7/fax[+]

(Liebl et al., 2000)

NOT Enhanced by

Statement

Reference

trio^M89/trio^S036810 has partially lethal - majority live phenotype, non-enhanceable by Df(2R)BSC273/+

(Dean et al., 2013)

trio^M89/trio^S036810 has some die during P-stage phenotype, non-enhanceable by Df(2R)BSC273/+

(Dean et al., 2013)

trio^M89/trio^S036810 has partially lethal - majority live phenotype, non-enhanceable by seq^vr5-5/seq[+]

(Dean et al., 2013)

trio^M89/trio^S036810 has some die during P-stage phenotype, non-enhanceable by seq^vr5-5/seq[+]

(Dean et al., 2013)

trio^M89/trio^S036810 has partially lethal - majority live phenotype, non-enhanceable by seq[+]/seq^vr5-48

(Dean et al., 2013)

trio^M89/trio^S036810 has some die during P-stage phenotype, non-enhanceable by seq[+]/seq^vr5-48

(Dean et al., 2013)

Enhancer of

Statement

Reference

trio^M89/trio^S036810 is an enhancer of abnormal neuroanatomy phenotype of fra⁴

(Forsthoefel et al., 2005)

trio[+]/trio^S036810 is an enhancer of abnormal neuroanatomy phenotype of fra⁴

(Forsthoefel et al., 2005)

Other

Statement

Reference

seq^9.17/seq[+], trio^M89/trio^S036810 has abnormal feeding behavior phenotype

(Dean et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

trio^M89/trio^S036810 has larval longitudinal connective phenotype, enhanceable by Abl¹/Abl[+]

(Liebl et al., 2000)

Enhancer of

Statement

Reference

trio^M89/trio^S036810 is an enhancer of symmetrical commissure phenotype of fra⁴

(Forsthoefel et al., 2005)

trio[+]/trio^S036810 is an enhancer of symmetrical commissure phenotype of fra⁴

(Forsthoefel et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

seq^9.17 dominantly exacerbates the pre-pupal lethality of trio^M89/trio^S036810. However, survival to third instar larvae is indistinguishable between wild-type and the mutants. The seq^9.17/+; trio^M89/trio^S036810 larvae retain the forager-like feeding behaviour at a time (90-93 hours after hatching) when their siblings and all control larvae have already transitioned to wanderers.

(Dean et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

trio^2p2.1

(Saito et al., 2002)

trio^2Z4.1

(Saito et al., 2002)

trio^2Y1.1

(Saito et al., 2002)