FlyBase Allele Report: Dmel\SmD3[k15401]

General Information

Symbol

Dmel\SmD3^k15401

Species

D. melanogaster

Name

FlyBase ID

FBal0100397

Feature type

allele

Associated gene

Dmel\SmD3

Associated Insertion(s)

P{lacW}SmD3^k15401

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Schenkel et al., 2002, Schenkel et al., 1999, BDGP Project Members, 1994-1999, Torok et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{lacW}SmD3^k15401

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Torok et al., 1993)

lethal - all die before end of larval stage (with SmD3^EP2176)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k09037)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k09540)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k11803)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k13107)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^lex47)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^ΔLR51)

(Schenkel et al., 2002)

lethal - all die before end of larval stage | recessive

(Schenkel et al., 2002)

some die during larval stage (with SmD3^EP2176)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k09037)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k09540)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k11803)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k13107)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^lex47)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^ΔLR51)

(Schenkel et al., 2002)

some die during larval stage | recessive

(Schenkel et al., 2002)

Phenotype Manifest In

embryonic/larval lymph gland

(Torok et al., 1993)

larval brain

(Torok et al., 1993)

Detailed Description

Statement

Reference

Brain, imaginal disc and blood cell neoplastic phenotype.

(Mechler, 1994)

Lethality occurs during larval and pupal stages. Brain is smaller than wild type with abnormal shape. Discs are smaller than wild type with abnormal folding. Both discs and brain can exceed normal size at the end of larval life. Lymph gland retains lobular structure.

(Torok et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

SmD3^ΔLR51

(Schenkel et al., 2002)

SmD3^EP2176

(Schenkel et al., 2002)

SmD3^lex47

(Schenkel et al., 2002)

SmD3^k09037

(Schenkel et al., 2002)

SmD3^k09540

(Schenkel et al., 2002)

SmD3^k11803

(Schenkel et al., 2002)

SmD3^k13107

(Schenkel et al., 2002)

Rescued by

SmD3^k15401 is rescued by SmD3^+t2.3

(Schenkel et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

102962

y¹ w^67c23 ; P{lacW}SmD3^k15401 / CyO

Notes on Origin

Discoverer

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

The P{lacW}SmD3^k15401 insertion appears to affect only SmD3 function and not Oda function (despite being within an Oda intron), since the lethality of P{lacW}SmD3^k15401 homozygotes is fully rescued by the P{SmD3.+t2.3} construct which contains the complete SmD3 gene. Complements: Oda^EP2104.

(Schenkel et al., 2002)

P-element excision demonstrates the insertion to be the cause of lethality.

(Torok et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

SmD3^k15401

guf^k15401

l(2)154/1

(Mechler, 1994, Torok et al., 1993)

l(2)k09037^k15401

l(2)k154-01

(Schenkel et al., 2002, Schenkel et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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