FlyBase Allele Report: Dmel\SmD3[k11803]

(Schenkel et al., 2002, Schenkel et al., 1999, Spradling et al., 1999, Salzberg et al., 1996, Kania et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999)

lethal - all die before end of larval stage (with SmD3^EP2176)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k09037)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k13107)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^k15401)

(Schenkel et al., 2002)

lethal - all die before end of larval stage (with SmD3^ΔLR51)

(Schenkel et al., 2002)

lethal - all die during embryonic stage (with SmD3^k09540)

(Schenkel et al., 2002)

lethal - all die during embryonic stage (with SmD3^lex47)

(Schenkel et al., 2002)

lethal - all die during embryonic stage | recessive

(Schenkel et al., 2002, Salzberg et al., 1996, Kania et al., 1995)

some die during embryonic stage (with SmD3^k09540)

(Schenkel et al., 2002)

some die during embryonic stage (with SmD3^lex47)

(Schenkel et al., 2002)

some die during embryonic stage | recessive

(Schenkel et al., 2002, Salzberg et al., 1996, Kania et al., 1995)

some die during larval stage (with SmD3^EP2176)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k09037)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k13107)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^k15401)

(Schenkel et al., 2002)

some die during larval stage (with SmD3^ΔLR51)

(Schenkel et al., 2002)

Phenotype Manifest In

chordotonal organ

(Schenkel et al., 2002)

embryonic visceral muscle cell

(Salzberg et al., 1996)

larval somatic muscle cell

(Salzberg et al., 1996)

larval ventral nerve cord

(Schenkel et al., 2002)

presumptive embryonic/larval central nervous system

(Salzberg et al., 1996)

presumptive embryonic/larval peripheral nervous system

(Salzberg et al., 1996, Kania et al., 1995)

Detailed Description

Statement

Reference

Mutant embryos show defects in the lateral chordotonal organs and the ventral nerve cord.

(Schenkel et al., 2002)

Smaller number of PNS neurons express 22C10 in homozygous embryos and PNS axons dispay severe defects in growth cone guidance and fasciculation. Most noticeable phenotype is splitting of the intersegmental nerve into two fascicles, the ISN 'loop' phenotype. Defects in the CNS are similar to those observed in the PNS. Numerous muscles are missing, displaced or exhibit aberrant shapes. Embryos also contain many mononucleate myoblasts that fail to fuse into muscle syncytia. Defects also in the visceral and cardiac musculature.

(Salzberg et al., 1996)

Homozygous embryos exhibit loss of neurons.

(Kania et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

SmD3^ΔLR51

(Schenkel et al., 2002)

SmD3^EP2176

(Schenkel et al., 2002)

SmD3^lex47

(Schenkel et al., 2002)

SmD3^k09037

(Schenkel et al., 2002)

SmD3^k09540

(Schenkel et al., 2002)

SmD3^k13107

(Schenkel et al., 2002)

SmD3^k15401

(Schenkel et al., 2002)

Rescued by

SmD3^k11803 is rescued by SmD3^+t2.3

(Schenkel et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

I. Kiss.

Comments

The P{lacW}SmD3^k11803 insertion appears to affect only SmD3 function and not Oda function (despite being within an Oda intron), since the lethality of P{lacW}SmD3^k11803 homozygotes is fully rescued by the P{SmD3.+t2.3} construct which contains the complete SmD3 gene. Complements: Oda^EP2104.

(Schenkel et al., 2002)

P{lacW} insertion can be excised and revert the lethal phenotype to wild type.

(Kania et al., 1995)

Precise or near precise excision of the P{lacW} insertion reverts both the lethality and the PNS phenotype, demonstrating the insertion causes the phenotype.

(Salzberg et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

118/3

(Salzberg et al., 1996, Kania et al., 1995)

SmD3^k11803

guf^P118-3

(Salzberg et al., 1996)

guf^k11803

l(2)k118-03

(Gonsalvez et al., 2010, Schenkel et al., 2002, Schenkel et al., 1999)

l(2)k11803

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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