FlyBase Allele Report: Dmel\ago[1]

General Information

Symbol

Dmel\ago¹

Species

D. melanogaster

Name

FlyBase ID

FBal0126458

Feature type

allele

Associated gene

Dmel\ago

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Moberg et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q1195term.

(Moberg et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,248,026..4,248,026 [-]

Nucleotide change:

C4248026T

Amino acid change:

Q1195term | ago-PA; Q1195term | ago-PB; Q1195term | ago-PC

Reported amino acid change:

Q1195term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Moberg et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval dorsal tracheal branch (with ago³)

(Mortimer and Moberg, 2007)

embryonic/larval ganglionic tracheal branch (with ago³)

(Mortimer and Moberg, 2007)

embryonic/larval ganglionic tracheal branch (with ago^Δ3-7)

(Mortimer and Moberg, 2013)

embryonic/larval tracheal dorsal trunk (with ago³)

(Mortimer and Moberg, 2009, Mortimer and Moberg, 2007)

embryonic/larval tracheal lateral trunk (with ago³)

(Mortimer and Moberg, 2007)

eye | somatic clone

(Moberg et al., 2004)

larval lateral G tracheal branch (with ago^Δ3-7)

(Mortimer and Moberg, 2013)

larval lateral H tracheal branch (with ago^Δ3-7)

(Mortimer and Moberg, 2013)

larval RP2 motor neuron | ectopic

(Bhat and Apsel, 2004)

larval tracheal system (with ago³)

(Mortimer and Moberg, 2007)

nurse cell & nucleus | germ-line clone

(Doronkin et al., 2003)

nurse cell | germline clone

(Doronkin et al., 2003)

tracheal fusion cell (with ago³)

(Mortimer and Moberg, 2007)

tracheal lumen (with ago³)

(Mortimer and Moberg, 2007)

tracheal primordium (with ago³)

(Mortimer and Moberg, 2007)

tracheole (with ago^Δ3-7)

(Mortimer and Moberg, 2013)

tracheole | increased number (with ago^Δ3-7)

(Mortimer and Moberg, 2013)

wing disc | somatic clone

(Moberg et al., 2004)

Detailed Description

Statement

Reference

ago¹/ago^Δ3-7 third instar larvae have tracheal defects, with the most prevalent phenotype being an approximate doubling of the number of cytoplasmic branches elaborated from multiple subtypes of terminal cells, including the lateral LH and LG terminal cells. Other defects include terminal branch tangles and the development of 'ringlet'-shaped ganglionic branches, which are seen in approximately 25% of larvae.

(Mortimer and Moberg, 2013)

ago¹/ago³ transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.

ago¹/Df(3L)Exel9000 transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.

ago¹/ago³ mutant embryos exhibit defects in the developing trachea. The earliest of these are interruptions or 'breaks' in the continuity of the tracheal lumen. Approximately 70% of these mutants display breaks throughout the tracheal system and are prominent in the dorsal trunk, the lateral trunk, and between dorsal branches of opposing tracheal placodes. The dorsal branches and ganglionic branches also appear to show misrouting. The second prominent tracheal phenotype (occurring in 25% of embryos) is excess lumen convolution through the primary and secondary branches. Mutants have approximately the same number of nuclei per dorsal trunk segment, indicating that the primary effect of ago loss in the dorsal trunk is not on cell number, but on fusion cell migration and fusion.

(Mortimer and Moberg, 2007)

In ago¹ homozygous embryos, 2% of hemisegments have two RP2 sibs and one RP2 neuron, while others have two RP2 neurons and one sib, rather than the one cell of each type seen in each segment in wild-type embryos.

(Bhat and Apsel, 2004)

Homozygous mutant clones in the wing disc and in the adult eye are consistently larger and contain more cells than their respective twin spots. Despite the faster rate of cell division in mutant cells, cells of homozygous wing disc clones are not decreased in size compared to control cells.

(Moberg et al., 2004)

When ago¹ mutant clones are induced in the ovary, some mutant cysts have only a single, large, highly polyploid germ-line cell.

(Doronkin et al., 2003)

Adult eyes mosaic for mutations in ago are composed mostly of mutant tissue, exhibiting a proliferative advantage over their wild-type twin-spots.

(Moberg et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ago^Δ3-7/ago¹ has lethal phenotype, suppressible | partially by sima[+]/sima^KG07607

(Mortimer and Moberg, 2013)

Suppressor of

Statement

Reference

ago[+]/ago¹ is a suppressor of female sterile | recessive phenotype of Myc^dm-1

(Moberg et al., 2004)

ago[+]/ago¹ is a suppressor of decreased cell number | recessive phenotype of Myc^dm-1

(Moberg et al., 2004)

ago[+]/ago¹ is a suppressor of decreased body size | recessive phenotype of Myc^dm-1

(Moberg et al., 2004)

ago[+]/ago¹ is a suppressor of decreased body size | recessive | female phenotype of Myc^dm-1

(Moberg et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^GMR.PU, ago¹ has eye phenotype, enhanceable by puf^EP3472, Scer\GAL4^GMR.PU

(Li et al., 2013)

Myc^UAS.cZa, Scer\GAL4^GMR.PU, ago¹ has eye phenotype, enhanceable by puf^EY03971, Scer\GAL4^GMR.PU

(Li et al., 2013)

ago³/ago¹ has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by Df(2R)Exel6060/+

(Mortimer and Moberg, 2009)

ago³/ago¹ has larval tracheal system phenotype, enhanceable by awd^j2A4/awd[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by awd^j2A4/awd[+]

(Mortimer and Moberg, 2007)

Suppressed by

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^GMR.PU, ago¹ has eye phenotype, suppressible by puf^A459

(Li et al., 2013)

ago^Δ3-7/ago¹ has tracheole | increased number phenotype, suppressible by sima[+]/sima^KG07607

(Mortimer and Moberg, 2013)

ago^Δ3-7/ago¹ has tracheole phenotype, suppressible by sima[+]/sima^KG07607

(Mortimer and Moberg, 2013)

ago^Δ3-7/ago¹ has larval lateral H tracheal branch phenotype, suppressible by sima[+]/sima^KG07607

(Mortimer and Moberg, 2013)

ago^Δ3-7/ago¹ has tracheole phenotype, suppressible by bnl[+]/bnl⁰⁰⁸⁵⁷

(Mortimer and Moberg, 2013)

ago^Δ3-7/ago¹ has larval lateral H tracheal branch phenotype, suppressible by bnl[+]/bnl⁰⁰⁸⁵⁷

(Mortimer and Moberg, 2013)

ago³/ago¹ has larval tracheal system phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval tracheal dorsal trunk phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal lumen phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval tracheal lateral trunk phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval dorsal tracheal branch phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal primordium phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval ganglionic tracheal branch phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal fusion cell phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal lumen phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval tracheal lateral trunk phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval dorsal tracheal branch phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal primordium phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval ganglionic tracheal branch phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has tracheal fusion cell phenotype, suppressible by trh¹⁰⁵¹²/trh[+]

(Mortimer and Moberg, 2007)

ago³/ago¹ has larval tracheal system phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

ago³/ago¹ has embryonic/larval tracheal dorsal trunk phenotype, suppressible by btl[+]/btl^dev1

(Mortimer and Moberg, 2007)

Enhancer of

Statement

Reference

ago[+]/ago¹ is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, puf^EY03971

(Li et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

sima^KG07607 dominantly delays the lethal phase of ago^Δ3-7/ago¹ transheterozygotes.

sima^KG07607 dominantly suppresses the excess and overlapping terminal tracheal branching seen in ago^Δ3-7/ago¹ larvae.

bnl⁰⁰⁸⁵⁷ dominantly suppresses the increase in number of terminal branches per lateral LH cell seen in ago¹/ago^Δ3-7 larvae.

(Mortimer and Moberg, 2013)

An ago¹ heterozygous background suppresses the CoVa^tend mutant clone adult eye phenotype.

(Mandal et al., 2010)

A trh¹⁰⁵¹² heterozygous background dominantly suppresses the tracheal phenotypes found in ago¹/ago³ mutants.

A btl^dev1 heterozygous background dominantly suppresses the tracheal phenotypes found in ago¹/ago³ mutants.

A awd^j2A4 heterozygous background strongly enhances both the penetrance (from 46% of embryos to greater than 80% of embryos) of the ago¹/ago³ mutant dorsal trunk phenotype and its expressivity among affected embryos. These embryos also show a much more severe disruption of the entire tracheal system compared to ago¹/ago³ mutants.

(Mortimer and Moberg, 2007)

Heterozygosity for ago¹ increase the female body length of dm¹ flies by about 12%. The wings of dm¹ ; ago¹/+ adults are about 15% larger than those of dm¹ adults. This suppression is due to an increase in the number of cells. dm¹ ; ago¹/+ females lay eggs that can give rise to viable larvae.

(Moberg et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ago³/ago¹ is rescued by ago^UAS.cMa/Scer\GAL4^btl.PS

(Mortimer and Moberg, 2007)

Comments

Expression of ago^Scer\UAS.cMa in the developing tracheal system under the control of Scer\GAL4^btl.PS in an ago¹/ago³ mutant background significantly reduces the frequency of dorsal trunk breaks, along with other tracheal phenotypes.

(Mortimer and Moberg, 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ago¹

(Vedelek et al., 2021, Kuang et al., 2020, Bivik Stadler et al., 2019, Suisse et al., 2017, Bivik et al., 2015, Bandura et al., 2013, Li et al., 2013, Mortimer and Moberg, 2013, Mandal et al., 2010, Mortimer and Moberg, 2009, Rhiner et al., 2009, Szuplewski et al., 2009, Mortimer and Moberg, 2007)

Name Synonyms

Secondary FlyBase IDs

References (18)

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