FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\par-1UAS.cSa
Open Close
General Information
Symbol
Dmel\par-1UAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sun
FlyBase ID
FBal0146942
Feature type
allele
Associated gene
Dmel\par-1
Associated Insertion(s)
Carried in Construct
P{UAS-par-1.S}
Also Known As
UAS-PAR-1, UAS-dpar-1
Key Links
Transgenic product class
wild_type
(Sun et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sun et al., 2001)
Mutagen
in vitro construct
(Sun et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-par-1.S}
Cytology
Description

UASt regulatory sequences drive expression of the full length α form of par-1.

(Sun et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
par-1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Nishimura et al., 2004)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal 4 ventral denticle belt, with Scer\GAL4VP16.mat.αTub67C

      (Sun et al., 2001)

      abdominal 5 ventral denticle belt, with Scer\GAL4VP16.mat.αTub67C

      (Sun et al., 2001)

      axon | larval stage, with Scer\GAL4BG380

      (Barber et al., 2017)

      axon | larval stage, with Scer\GAL4elav.PU

      (Barber et al., 2017)

      axon | larval stage, with Scer\GAL4nSyb.PU

      (Barber et al., 2017)

      embryonic/larval neuromuscular junction, with Scer\GAL4Mhc.PW

      (Zhang et al., 2007)

      embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4BG380

      (Barber et al., 2017)

      eye, with Scer\GAL4elav-C155

      (Nishimura et al., 2004)

      eye, with Scer\GAL4GMR.PF

      (Wu et al., 2011)

      eye, with Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      eye, with Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      eye, with Scer\GAL4hs.PB

      (Nishimura et al., 2004)

      eye photoreceptor cell, with Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      interommatidial bristle, with Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      interommatidial bristle, with Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      microtubule, with Scer\GAL4C-765

      (Harumoto et al., 2010)

      NMJ bouton, with Scer\GAL4Mhc.PW

      (Zhang et al., 2007)

      NMJ bouton | larval stage, with Scer\GAL4BG380

      (Barber et al., 2017)

      ommatidium, with Scer\GAL4GMR.PF

      (Wu et al., 2011)

      ommatidium, with Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      ommatidium, with Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      photoreceptor cell & neuron, with Scer\GAL4elav-C155

      (Nishimura et al., 2004)

      photoreceptor cell & neuron, with Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      photoreceptor cell & neuron, with Scer\GAL4hs.PB

      (Nishimura et al., 2004)

      presynaptic active zone, with Scer\GAL4Mhc.PW

      (Zhang et al., 2007)

      presynaptic active zone | larval stage, with Scer\GAL4BG380

      (Barber et al., 2017)

      subsynaptic reticulum, with Scer\GAL4Mhc.PW

      (Zhang et al., 2007)

      synaptic vesicle, with Scer\GAL4Mhc.PW

      (Zhang et al., 2007)

      T-bar | larval stage, with Scer\GAL4BG380

      (Barber et al., 2017)

      wing hair, with Scer\GAL4C-765

      (Harumoto et al., 2010)
      Detailed Description
      Statement
      Reference

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4BG380, Scer\GAL4nSyb.PU or Scer\GAL4elav.PU, but not Scer\GAL4G7, in larvae leads to specific accumulation of a subset of synaptic cargo in axons, as compared to controls.

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4BG380 in larval neuromuscular junctions leads to a decrease in the number of active zones at the synapses, a decrease in the number of T-bars per active zone at the synapses, a decrease in the size of synaptic boutons, a reduction in the frequency (but not the amplitude) of mini excitatory junction potentials (mEJP), a reduction in excitatory junction potential (EJP) amplitude, and a decrease in quantal content, but does not affect synaptic span or density, and does not affect axonal mitochondrial transport nor the levels or size of mitochondria, as compared to controls.

      (Barber et al., 2017)

      Overexpression of par-1Scer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PF leads to a rough-eye phenotype.

      (Wu et al., 2011)

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4C-765 results in misorientation of wing hairs primarily in a proximal region up to the L3-1 location (position of the most proximal dorsal campaniform sensillum on vein L3), with wing hair polarity being normal more distal to the L3-1 location. These animals show defects in the directional preferences of growing microtubules in wing cells 24 hours after puparium formation; in the proximal region up to the L3-1 location, subpopulations of proximodistal oriented microtubules are decreased with a concomitant increase in those of anteroposterior oriented ones, and in addition the proximodistal asymmetry of microtubule polarity is milder than in wild type and is no longer statistically significant in the mutant animals.

      (Harumoto et al., 2010)

      Adult eyes expressing par-1Scer\UAS.cSa under the control of Scer\GAL4GMR.PS display a reduced, rough-eye phenotype with disordered ommatidia and missing bristles. Retinal sections show disorganized ommatidia with marked disorganization of photoreceptor neurons.

      (Chatterjee et al., 2009)

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW results in an approximately 60% decrease in the number of type I boutons at the neuromuscular junction of muscle 6/7 and the overall structure of the synaptic nerve terminals is simplified compared to controls. There is a dramatic loss of subsynaptic reticulum in the mutant boutons compared to controls and the mutant presynaptic regions show a moderate decrease in synaptic vesicle density and active zone number.

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4elav.PU has no significant effect on the number of boutons at the neuromuscular junction of muscle 6/7.

      The frequency and amplitude of the miniature excitatory junctional current are reduced by 60% and 40% respectively compared to controls in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW. The amplitude of the evoked excitatory junctional current is reduced by 44% compared to controls in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW. The quantal content is not significantly altered in these animals.

      (Zhang et al., 2007)

      Flies expressing a strong par-1Scer\UAS.cSa expression line under the control of Scer\GAL4hs.2sev have severely reduced eyes, with fused ommatidia and missing interommatidial bristles. Flies expressing a weak or mild par-1Scer\UAS.cSa expression line under the control of Scer\GAL4hs.2sev have slightly reduced eyes which appear rough and have disordered ommatidia and occasional missing bristles. Flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4hs.2sev show loss of photoreceptor neurons (a 0.9% loss in photoreceptor neurons is seen in a weak par-1Scer\UAS.cSa expression line). Flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4elav-C155 have a mild rough eye phenotype and show loss of photoreceptor neurons. Flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4hs.PB (using daily heat shock treatment starting from the first larval instar) have rough eyes and show loss of photoreceptor neurons. Heat shock starting from pupal or young adult stages also results in a photoreceptor loss phenotype in these flies. Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4Cha.7.4 has little effect on the cholinergic neurons.

      (Nishimura et al., 2004)

      Expression of par-1Scer\UAS.cSa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in an cuticle phenotype in which the 4th or 5th abdominal denticle belt is replaced by naked cuticle.

      (Sun et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PF/par-1UAS.cSa is an enhancer of eye phenotype of Hsap\DAPK1UAS.cWa

      (Wu et al., 2011)

      par-1UAS.cSa, Scer\GAL4GMR.PF is an enhancer of ommatidium phenotype of Hsap\DAPK1UAS.cWa, Scer\GAL4GMR.PF

      (Wu et al., 2011)

      Scer\GAL4GMR.PF/par-1UAS.cSa is an enhancer of eye phenotype of Hsap\DAPK1K42A.UAS

      (Wu et al., 2011)

      par-1UAS.cSa, Scer\GAL4GMR.PF is an enhancer of ommatidium phenotype of Hsap\DAPK1K42A.UAS, Scer\GAL4GMR.PF

      (Wu et al., 2011)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of ommatidium phenotype of Hsap\MAPTUAS.wt, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of eye phenotype of Hsap\MAPTUAS.wt, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of interommatidial bristle phenotype of Hsap\MAPTUAS.wt, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of rhabdomere phenotype of Hsap\MAPTUAS.wt, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of interommatidial bristle phenotype of Hsap\MAPTUAS.S2A, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of eye phenotype of Hsap\MAPTUAS.S2A, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4GMR.PS is an enhancer of retina phenotype of Hsap\MAPTUAS.S2A, Scer\GAL4GMR.PS

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of ommatidium phenotype of Hsap\MAPTGMR.Ex.PJ

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of eye phenotype of Hsap\MAPTGMR.Ex.PJ

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of interommatidial bristle phenotype of Hsap\MAPTGMR.Ex.PJ

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of rhabdomere phenotype of Hsap\MAPTGMR.Ex.PJ

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of ommatidium phenotype of Hsap\MAPTGMR.S11A

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of eye phenotype of Hsap\MAPTGMR.S11A

      (Chatterjee et al., 2009)

      Scer\GAL4GMR.PS/par-1UAS.cSa is an enhancer of interommatidial bristle phenotype of Hsap\MAPTGMR.S11A

      (Chatterjee et al., 2009)

      par-1UAS.cSa, Scer\GAL4hs.2sev is an enhancer of eye phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      par-1UAS.cSa, Scer\GAL4hs.2sev is an enhancer of eye phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      par-1UAS.cSa, Scer\GAL4hs.2sev is an enhancer of eye photoreceptor cell & neuron phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4hs.2sev

      (Nishimura et al., 2004)

      par-1UAS.cSa, Scer\GAL4ChAT.7.4 is an enhancer of neuron phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4ChAT.7.4

      (Nishimura et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tauMR22/+ fails to suppress the axonal transport defects seen in flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4BG380.

      (Barber et al., 2017)

      dlg17/+ enhances the reduction in the number boutons at the neuromuscular junction which is seen in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW.

      Co-expression of either dlg1WT.Scer\UAS.T:Avic\GFP or dlg1SA.Scer\UAS.T:Avic\GFP partially suppresses the the decrease in the number of boutons at the neuromuscular junction which is seen in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW.

      Co-expression of dlg1SD.Scer\UAS.T:Avic\GFP does not suppress the decrease in the number of boutons at the neuromuscular junction which is seen in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW.

      The loss of subsynaptic reticulum and reduction in synaptic vesicle density and active zone number that is seen at the neuromuscular junction in animals expressing par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW is largely rescued by co-expression of dlg1SA.Scer\UAS.T:Avic\GFP.

      The electrophysiological defects caused by expression of par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW are suppressed by co-expression of dlg1WT.Scer\UAS.T:Avic\GFP.

      The electrophysiological defects caused by expression of par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW are not suppressed by co-expression of dlg1SD.Scer\UAS.T:Avic\GFP.

      The defects in the frequency and amplitude of the miniature excitatory junctional current which are caused by expression of par-1Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW are suppressed by co-expression of dlg1SA.Scer\UAS.T:Avic\GFP. The double mutant animals show a slight increase in the amplitude of the evoked excitatory junctional current compared to wild-type controls, and the quantal content is also higher than normal.

      (Zhang et al., 2007)

      The eye degeneration phenotype seen in flies expressing a strong par-1Scer\UAS.cSa expression line under the control of Scer\GAL4hs.2sev is partly suppressed by Df(3R)R97/+.

      (Nishimura et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Coexpression of a low-expression Hsap\DAPK1Scer\UAS.cWa with par-1Scer\UAS.cSa in the developing eye, both under the control of Scer\GAL4GMR.PF, produces a synergistic interaction, resulting in markedly reduced eye size with fewer ommatidia.

      Coexpression of a low-expression Hsap\DAPK1K42A.Scer\UAS with par-1Scer\UAS.cSa in the developing eye, both under the control of Scer\GAL4GMR.PF, produces a synergistic interaction, resulting in markedly reduced eye size with fewer ommatidia.

      (Wu et al., 2011)

      The rough-eye phenotype produced by Scer\GAL4GMR.PS-mediated expression of Hsap\MAPTScer\UAS.wt is stronger when par-1Scer\UAS.cSa is co-expressed; the mild disorganization of the retina observed in Scer\GAL4GMR.PS, Hsap\MAPTScer\UAS.wt eyes is dramatically increased, resulting in smaller eyes with more missing bristles and fused ommatidia. Co-expression of par-1Scer\UAS.cSa also increases the severity of the ommatidial polarity and rhabdomere loss phenotypes seen in Scer\GAL4GMR.PS, Hsap\MAPTScer\UAS.wt retinas. These effects may be additive rather than synergistic.

      Coexpression of par-1Scer\UAS.cSa results in only a modest worsening of the Hsap\MAPTScer\UAS.S2A, Scer\GAL4GMR.PS rough-eye phenotype; the double transgenics have slightly more irregular eyes with disordered and occasional missing bristles. The internal retinal morphology of flies coexpressing par-1Scer\UAS.cSa and Hsap\MAPTScer\UAS.S2A is only slightly worse than that of flies expressing Hsap\MAPTScer\UAS.S2A alone, with slightly misshapen rhabdomeres and a moderate loss of photoreceptor neurons.

      The eye and retinal phenotypes seen in Hsap\MAPTGMR.Ex.PJ animals are enhanced by Scer\GAL4GMR.PS-mediated co-expression of par-1Scer\UAS.cSa.

      The Hsap\MAPTGMR.S11A small and rough eye phenotype is enhanced by Scer\GAL4GMR.PS-mediated co-expression of par-1Scer\UAS.cSa. Retinas of the double transgenics show dramatic disruption.

      (Chatterjee et al., 2009)

      Co-expression of a weak par-1Scer\UAS.cSa expression line enhances the eye phenotype caused by expression of Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4hs.2sev, resulting in smaller eyes. A 15% loss in photoreceptor neurons is seen in these flies. Co-expression of par-1Scer\UAS.cSa and Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4ChAT.7.4 results in profound vacuole formation in the cell bodies and neuronal processes of the cholinergic neurons. Co-expression of Hsap\MAPTScer\UAS.cWa and par-1Scer\UAS.cSa under the control of Scer\GAL4hs.2sev exacerbates the eye phenotype compared to expression of Hsap\MAPTScer\UAS.cWa or par-1Scer\UAS.cSa alone. Co-expression of Hsap\MAPTR406W.S2A.Scer\UAS.0N4R in flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4hs.2sev results in a mild rough eye phenotype similar to that seen in flies expressing par-1Scer\UAS.cSa under the control of Scer\GAL4hs.2sev alone.

      (Nishimura et al., 2004)
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4GMR57C10/par-1UAS.cSa partially rescues par-1Δ-16

      (Herzmann et al., 2017)
      Comments

      The pruning defects observed in class IV dendritic arborizing neuron par-1Δ-16 mutant MARCM clones can be rescued by expression of par-1Scer\UAS.cSa (with Scer\GAL4GMR57C10) in the mutant clones.

      (Herzmann et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PAR-1RL
      (Herzmann et al., 2017)
      par-1Scer\UAS.cSa
      par-1UAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Sun
      (Sun et al., 2001)
      Secondary FlyBase IDs
        References (12)