FlyBase Allele Report: Dmel\Itpr[wc703]

General Information

Symbol

Dmel\Itpr^wc703

Species

D. melanogaster

Name

FlyBase ID

FBal0156338

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Deshpande et al., 2000)

Mutagen

ethyl methanesulfonate

(Joshi et al., 2004, Deshpande et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: G2117E. Nucleotide substitution: G?A.

(Joshi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:5,536,153..5,536,153 [+]

Nucleotide change:

G5536153A

Reported nucleotide change:

G?A

Amino acid change:

G2114E | Itpr-PA; G2123E | Itpr-PB; G2114E | Itpr-PC

Reported amino acid change:

G2117E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Joshi et al., 2004, Deshpande et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autosomal dominant cerebellar ataxia

CEC with Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Psn^B3

(McBride et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

abnormal flight (with Itpr^wc361)

(Banerjee et al., 2004)

abnormal learning | adult stage | male

(McBride et al., 2010)

abnormal neurophysiology (with Itpr^ug3)

(Agrawal et al., 2010, Banerjee et al., 2004)

abnormal starvation stress response (with Itpr^ug3)

(Subramanian et al., 2013)

flightless (with Itpr^ug3)

(Banerjee et al., 2004)

flightless | partially (with Itpr^wc361)

(Banerjee et al., 2006)

lethal | cold sensitive (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020, Joshi et al., 2004)

lethal | recessive

(Joshi et al., 2004)

partially lethal | cold sensitive (with Itpr^wc361)

(Joshi et al., 2004)

partially lethal - majority live (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

viable (with Itpr^wc361)

(Banerjee et al., 2006)

visible (with Itpr^ug3)

(Agrawal et al., 2010, Banerjee et al., 2004)

visible | adult stage (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020)

Phenotype Manifest In

indirect flight muscle cell (with Itpr^ug3)

(Banerjee et al., 2004)

wing (with Itpr^ug3)

(Balasubramanian and Srinivasan, 2020, Agrawal et al., 2010, Banerjee et al., 2004)

Detailed Description

Statement

Reference

Itpr^ug3/Itpr^wc703 adults show a "spread out" wing posture.

(Balasubramanian and Srinivasan, 2020)

itgr[wu] flies (Itp-r83A^wc703/Itp-r83A^ug3) are resistant to starvation, but show a wild type response to oxidative stress. Females survive longer than males.

(Subramanian et al., 2013)

Itp-r83A^wc703/Itp-r83A^ug3 animals have wing posture, flight and flight physiology defects.

(Agrawal et al., 2010)

Itp-r83A^wc703/+ 30-day-old adult males display impaired (but not abrogated) learning ability as they demonstrate only a weak decrease in courtship activity when paired with an mated unreceptive female but their short-term (60 min) memory is not impaired.

(McBride et al., 2010)

The spontaneous firing rate seen in dorsal-longitudinal muscles of Itp-r83A^wc361/Itp-r83A^wc703 adults is normal. These adults can be divided into fliers and non-fliers (assayed using a cylinder drop test). About 30% are non-fliers - exhibiting only brief rhythmic flight patterns that are usually not sustained beyond 5 seconds in response to stimulation with an air puff. In contrast, the non-fliers exhibit sustained flight patterns (>30seconds) in response to the same stimulus.

(Banerjee et al., 2006)

Itp-r83A^wc703/Itp-r83A^ug3 flies show a "down-turned" wing phenotype.

Itp-r83A^wc703/Itp-r83A^wc361 flies show a significant degree of flightlessness but show normal wing posture.

Indirect flight muscles of wild-type flies can express short bursts of electrical activity that are associated with cleaning behavior. Itp-r83A^wc703/Itp-r83A^ug3 flies show an increase in spontaneous firing from these muscles, which is unrelated to cleaning behavior. The indirect flight muscles of these mutants fail to produce firing patterns in response to an air puff, which occurs in wild-type muscles.

(Banerjee et al., 2004)

Maintenance of adaptation to a butanol source in Itp-r83A^wc703 mutants is strongly defective, and recovery to 50% of preadaptation response occurs in less than one-third of the time taken in wild-type. The dominant phenotype of Itp-r83A^wc703 is further accentuated by the presence of the recessive alleles Itp-r83A^wc361 and Itp-r83A^ug3. This suggests that the Itp-r83A dosage affects recovery from adaptation.

(Deshpande et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, enhanceable by Gα49B[+]/Gαq^221c

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Itpr^wc703/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype, suppressible | partially by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has visible | adult stage phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has visible | adult stage phenotype, suppressible by NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by Orai[+]/Orai^k11505

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/NUCB1^c01508/SERCA^Kum170/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by NUCB1^c01508/Orai[+]/Orai^k11505/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has increased mortality during development | cold sensitive phenotype, suppressible by NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, suppressible by Orai[+]/Orai^k11505, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, suppressible by SERCA[+]/Orai[+]/Orai^k11505/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, suppressible by Orai[+]/NUCB1^NIG.32190R/Orai^k11505/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, suppressible by NUCB1^c01508/Orai[+]/Orai^k11505/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703 has abnormal learning | male | adult stage phenotype, suppressible by Psn^B3/Psn[+]

(McBride et al., 2010)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has flightless | partially phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has abnormal neurophysiology phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has flightless | partially phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

NOT suppressed by

Statement

Reference

Itpr^wc703/Itpr^ug3, Orai^k11505, SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/NUCB1^c01508/SERCA^Kum170/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by NUCB1^NIG.32190R/Scer\GAL4^αTub84B.PL

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has abnormal flight phenotype, non-suppressible by Orai[+]/Orai^k11505

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703 has abnormal learning | male | adult stage phenotype, non-suppressible by Psn^I2/Psn[+]

(McBride et al., 2010)

Suppressor of

Statement

Reference

Itpr^wc703/Itp-r83A[+] is a suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^wc703/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^wc703/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

NOT Suppressor of

Statement

Reference

Itpr^wc703/Itp-r83A[+] is a non-suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

Other

Statement

Reference

Itpr^wc703/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3, SERCA^Kum170 has viable phenotype

(Balasubramanian and Srinivasan, 2020)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has partially lethal - majority die phenotype

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has abnormal neurophysiology phenotype

(Banerjee et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, enhanceable by Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Suppressed by

Statement

Reference

Itpr^wc703/Itpr^ug3 has wing phenotype, suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3 has wing phenotype, suppressible by NUCB1^NIG.32190R/Scer\GAL4^Ddc.PL

(Balasubramanian and Srinivasan, 2020)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype, suppressible | partially by Gαq^UAS.cRa/Scer\GAL4^Ddc.PL

(Banerjee et al., 2006)

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+] has wing phenotype, suppressible by Ca-P60A[+]/SERCA^Kum170

(Banerjee et al., 2006)

Other

Statement

Reference

Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361 has wing phenotype

(Banerjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The learning defects (measured in a courtship behavior assay) observed in the Psn^B3, Psn^I2 or the Itp-r83A^wc703 single heterozygote 30 day-old males are suppressed in the Psn^B3/+;Itp-r83A^wc703/+ but not in the Psn^I2/+;Itp-r83A^wc703/+ double heterozygotes. Combination with a single copy of Itp-r83A^90B.0 can however restore the short-term memory defects characteristic for both Psn^B3 and Psn^I2 aged heterozygote males.

(McBride et al., 2010)

The viability of Itp-r83A^wc703/Itp-r83A^wc361 animals is unaffected by sl¹/sl¹ or sl²/sl². It is also unaffected by Gα49B^221c/+, but the resulting adults have abnormal wing posture, an enhanced incidence of flight defects and mild endogenous hyperactivity of spontaneous firing recorded from the dorsal longitudinal muscles. Wing posture and flight defect phenotypes are enhanced and viability is reduced if Plc21C^k31911/+ is also present, but partially suppressed by Gα49B^Scer\UAS.cRa; Scer\GAL4^Ddc.PL and completely or largely suppressed by Ca-P60A^Kum170/+, even when Plc21C^k31911/+ is also present.

(Banerjee et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Itpr⁰⁵⁶¹⁶

(Joshi et al., 2004)

Itpr^90B.0

(Joshi et al., 2004)

Itpr^B4

(Joshi et al., 2004)

Itpr^XR12

(Joshi et al., 2004)

Itpr^ug3

(Deshpande et al., 2000)

Itpr^wc361

(Deshpande et al., 2000)

Rescued by

Itpr^wc703/Itpr^ug3 is rescued by Scer\GAL4^hs.PB/Itpr^UAS.cVa

(Banerjee et al., 2004)

Itpr^wc703/Itpr^ug3 is rescued by Scer\GAL4^Ddc.PL/Itpr^UAS.cVa

(Banerjee et al., 2004)

Partially rescued by

Itpr^wc703/Itpr^ug3 is partially rescued by Scer\GAL4^Ilp2.PR/Itpr^UAS.cVa

(Agrawal et al., 2010)

Not rescued by

Itpr^wc703/Itpr^ug3 is not rescued by Scer\GAL4^elav.PLu/Itpr^UAS.cVa

(Banerjee et al., 2004)

Itpr^wc703/Itpr^ug3 is not rescued by Scer\GAL4^ChAT.7.4/Itpr^UAS.cVa

(Banerjee et al., 2004)

Itpr^wc703/Itpr^ug3 is not rescued by Scer\GAL4^RapGAP1-OK6/Itpr^UAS.cVa

(Banerjee et al., 2004)

Itpr^wc703/Itpr^ug3 is not rescued by Scer\GAL4^{shakB.lethal.4.1}/Itpr^UAS.cVa

(Banerjee et al., 2004)

Itpr^wc703/Itpr^ug3 is not rescued by Scer\GAL4^Mef2.PR/Itpr^UAS.cVa

(Banerjee et al., 2004)

Comments

Complete rescue of the altered wing posture is seen in Itp-r83A^Scer\UAS.cVa; Scer\GAL4^Ilp2.PR/+; Itp-r83A^wc703/Itp-r83A^ug3 animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A^wc703/Itp-r83A^ug3 flies expressing Scer\GAL4^Ilp2.PR-driven Itp-r83A^Scer\UAS.cVa leads to rhythmic generation of action potentials, but in most flies the air puff response lasts for a shorter period of time compared with the control flies.

(Agrawal et al., 2010)

Rescue of Itp-r83A^wc703/Itp-r83A^ug3 flies by expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^hs.PB following a heat shock shows that expression during early pupal development is sufficient to rescue flight behavior and physiology, while expression after adult emergence is ineffective in rescuing flight.

(Banerjee et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series: Itp-r83A^sv35 >= Itp-r83A^ka901 > Itp-r83A^sa54 >= Itp-r83A^ka1091 > Itp-r83A^wc703 > Itp-r83A^ug3 > Itp-r83A^wc361.

(Joshi et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

G2117E

(Chakraborty et al., 2016)

InsP₃R⁷⁰³

(McBride et al., 2010)

Itp-r83A^wc703

Itpr^wc703

itpr^wc703

(Balasubramanian and Srinivasan, 2020, Subramanian et al., 2013, Agrawal et al., 2010, Banerjee et al., 2006, Banerjee et al., 2004, Srikanth et al., 2004)

wc703

(Joshi et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (10)

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