FlyBase Allele Report: Dmel\Vps25[N55]

General Information

Symbol

Dmel\Vps25^N55

Species

D. melanogaster

Name

FlyBase ID

FBal0194622

Feature type

allele

Associated gene

Dmel\Vps25

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Herz et al., 2006)

Mutagen

ethyl methanesulfonate

(Herz et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q93term.

(Herz et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:8,625,854..8,625,854 [+]

Nucleotide change:

C8625854T

Amino acid change:

Q93term | Vps25-PA

Reported amino acid change:

Q93term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Herz et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA

(Woodfield et al., 2013, Herz et al., 2006)

model of cancer

CEA

(Herz et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of carcinoma

is exacerbated by Dark^H16

(Woodfield et al., 2013, Herz et al., 2006)

is exacerbated by bsk^DN.UAS.cUa

(Woodfield et al., 2013)

is exacerbated by Diap1^UAS.cHa

(Herz et al., 2006)

is ameliorated by N^DN.UAS.cUa

(Herz et al., 2006)

is exacerbated by hpo^3D

(Herz et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth | cell non-autonomous | somatic clone

(Herz et al., 2006)

abnormal size | adult stage (with Vps25^K2)

(Herz et al., 2006)

decreased cell number | pupal stage | somatic clone

(Herz et al., 2006)

hyperplasia | cell non-autonomous | somatic clone

(Herz et al., 2009)

increased cell death | larval stage | somatic clone

(Herz et al., 2006)

increased cell death | somatic clone

(Herz et al., 2006)

increased cell death | somatic clone | third instar larval stage

(Herz et al., 2009)

increased occurrence of cell division | cell non-autonomous | larval stage

(Herz et al., 2009)

increased occurrence of cell division | cell non-autonomous | larval stage | somatic clone

(Herz et al., 2006)

increased occurrence of cell division | cell non-autonomous | third instar larval stage

(Shravage et al., 2013)

lethal (with Vps25^K2)

(Herz et al., 2006)

neoplasia | third instar larval stage (with Vps25^K2)

(Herz et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Vps25^N55 mutant clones in late third instar larval eye imaginal discs causes non-autonomous overproliferation of neighbouring wild type cells.

(Shravage et al., 2013)

Vps25^N55 somatic clones in eye-antennal imaginal discs contain enlarged early endosomes.

Vps25^N55 somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. Non-autonomous tissue overgrowth of the eye-antennal imaginal disc, adult eye and head occurs these animals.

When the majority of cells in the eye-antennal imaginal discs are mutant for Vps25^N55, striking tissue overgrowth of the disc occurs and loose cellular architecture is observed.

Vps25^N55 mutant somatic clones in eye-antennal imaginal discs do not proliferate very well, however the wildtype tissue immediately adjacent to the mutant clones show increased cell proliferation rates.

(Herz et al., 2009)

Vps25^N55/Vps25^K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type.

TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25^N55 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25^N55 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages.

Cell proliferation is significantly increased in tissue adjacent to Vps25^N55 clones. This non-autonomous cell proliferation is best visible in wing imaginal discs, where Vps25^N55 clones appear to be the origin for the increased proliferation of the adjacent tissue.

Vps25^N55 eye-antennal disc mutant clones are unable to differentiate.

Abnormal endosomal structures are found in Vps25^N55 mutant clones concurrent with increased ubiquitin and N-signaling activity.

(Herz et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Vps25^N55 has abnormal cell growth | cell non-autonomous | somatic clone phenotype, non-enhanceable by N^DN.UAS.cUa/Scer\GAL4^Tub.PU

(Herz et al., 2006)

Suppressed by

Statement

Reference

Vps25^N55 has hyperplasia | somatic clone | cell non-autonomous phenotype, suppressible by N[+]/N^264-39

(Herz et al., 2009)

Vps25^N55 has increased cell death | somatic clone phenotype, suppressible by N^DN.UAS.cUa/Scer\GAL4^Tub.PU

(Herz et al., 2006)

Vps25^N55 has increased cell death | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/Diap1^UAS.cHa

(Herz et al., 2006)

Vps25^N55 has increased cell death | somatic clone | larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/Diap1^UAS.cHa

(Herz et al., 2006)

Vps25^N55 has increased cell death | somatic clone phenotype, suppressible by hpo^3D

(Herz et al., 2006)

Vps25^N55 has increased cell death | somatic clone | larval stage phenotype, suppressible by hpo^3D

(Herz et al., 2006)

NOT suppressed by

Statement

Reference

Vps25^N55 has increased cell death | somatic clone | larval stage phenotype, non-suppressible by N^DN.UAS.cUa/Scer\GAL4^Tub.PU

(Herz et al., 2006)

Vps25^N55 has increased occurrence of cell division | somatic clone | cell non-autonomous | larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Diap1^UAS.cHa

(Herz et al., 2006)

Suppressor of

Statement

Reference

Vps25^N55 is a suppressor of increased cell death phenotype of hid^GMR.PG

(Herz et al., 2006)

Other

Statement

Reference

Dark^H16, Vps25^N55 has abnormal cell growth | somatic clone | cell non-autonomous phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55 has increased occurrence of cell division | somatic clone | larval stage phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55 has neoplasia | somatic clone | third instar larval stage phenotype

(Herz et al., 2006)

Scer\GAL4^Tub.PU, Vps25^N55, puc^UAS.cMa has neoplasia | somatic clone | third instar larval stage phenotype

(Herz et al., 2006)

Diap1^UAS.cHa, Scer\GAL4^Tub.PU, Vps25^N55 has abnormal cell growth | somatic clone | cell non-autonomous phenotype

(Herz et al., 2006)

Diap1^UAS.cHa, Scer\GAL4^Tub.PU, Vps25^N55 has neoplasia | third instar larval stage phenotype

(Herz et al., 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Vps25^N55 has eye disc | somatic clone phenotype, non-enhanceable by N^DN.UAS.cUa/Scer\GAL4^Tub.PU

(Herz et al., 2006)

Suppressed by

Statement

Reference

Vps25^N55 has eye disc | somatic clone | cell non-autonomous phenotype, suppressible by Atg6¹

(Shravage et al., 2013)

Vps25^N55 has eye | somatic clone phenotype, suppressible by N[+]/N^264-39

(Herz et al., 2009)

Vps25^N55 has adult head | somatic clone phenotype, suppressible by N[+]/N^264-39

(Herz et al., 2009)

Suppressor of

Statement

Reference

Vps25^N55 is a suppressor of eye phenotype of hid^GMR.PG

(Herz et al., 2009, Herz et al., 2006)

Vps25^N55/Vps25^K2 is a suppressor of eye | somatic clone | cell non-autonomous phenotype of hid^GMR.PG

(Herz et al., 2006)

Other

Statement

Reference

Dark^H16, Vps25^N55 has eye phenotype

(Herz et al., 2006)

Dark^H16, Scer\GAL4^Tub.PU, Vps25^N55, puc^UAS.cMa has eye phenotype

(Herz et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Atg6¹ suppresses the non-autonomous overgrowth seen in Vps25^N55 mutant eye disc clones.

(Shravage et al., 2013)

Heterozygosity for N^264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of Vps25^N55 generated in the eye-antennal disc.

The eye-ablation phenotype in W^GMR.PG animals is suppressed in Vps25^N55 somatic clones generated in the eye-antennal imaginal disc in a non-autonomous manner.

(Herz et al., 2009)

Vps25^N55 moderate-strongly suppresses the W^GMR.PG eye phenotype.

In Vps25^N55/Vps25^K2 mosaic eyes, in a W^GMR.PG background, the rescued tissue is genetically wild-type or heterozygous, suggesting that Vps25^N55 and Vps25^K2 mutant tissue does not contribute to the suppression of W^GMR.PG.

Vps25^N55/N^DN.Scer\UAS mutants (using the MARCM technique) exhibit reduced or absent Stat92E activity. Cell proliferation is not significantly increased in Vps25^N55/N^DN.Scer\UAS mutants, with eye imaginal discs appearing normal in shape and size. These Vps25^N55/N^DN.Scer\UAS double mutant clones exhibit the same level of apoptosis as Vps25^N55 single mutant clones.

Blocking cell death, through expression of th^Scer\UAS.cHa in Vps25^N55 mutant clones does not affect proliferation or Stat92E activity.

Vps25^N55/th^Scer\UAS.cHa mosaics exhibit non-autonomous cell proliferation, as do Vps25^N55/Ark^H16 mutants. Eye-antennal discs of Vps25^N55/th^Scer\UAS.cHa mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25^N55/th^Scer\UAS.cHa mosaics. Vps25^N55/Ark^H16 cells undergo apoptosis. Vps25^N55/Ark^H16 clones cannot be recovered in the adult eye. Vps25^N55/th^Scer\UAS.cHa and Vps25^N55/Ark^H16 clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. The adult eye of Vps25^N55/Ark^H16 mosaics is severely overgrown and folded. Thus, inhibiting cell death in Vps25^N55 clones can give rise to an even stronger overgrowth phenotype.

Vps25^N55 Ark^H16/puc^Scer\UAS.cMa mosaic eye discs are severely overgrown.

Vps25^N55 hpo^3D mutant clones do not undergo apoptosis.

(Herz et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vps25^N55 is rescued by Vps25^UAS.cHa

(Herz et al., 2006)

Vps25^N55 is rescued by Vps25^hs.PH

(Herz et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

N55

(Herz et al., 2006)

Vps25^N55

(Wang et al., 2022)

Vps25ⁿ⁵⁵

(Shravage et al., 2013)

su(GMR-hid)N55

(Herz et al., 2006)

vps 25⁵⁵

(Childress et al., 2006)

vps25^N55

(Woodfield et al., 2013, Herz et al., 2009, Herz et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close