FlyBase Allele Report: Dmel\Rab5[GD10492]

Expressing Rab5^GD10492 under the control of Scer\GAL4^C587 leads to ectopic transiently amplifying spermatogonia and some dissociated somatic cells in L3 male gonads.

(La Marca et al., 2019)

In pupae expression of Rab5^GD10492 under the control of Scer\GAL4^GMR.long leads to proper rhabdomere and stalk segregation similar to controls.

(Ogi et al., 2019)

Animals expressing Rab5^GD10492 under the control of Scer\GAL4^grh.D4 are lethal, show defects in larval dorsal trunk elongation (resulting in internalization of posterior spiracles into the body cavity) and the larvae are smaller than age-matched controls.

(Wingen et al., 2017)

Expression of Rab5^GD10492 under the control of Scer\GAL4^tj-NP1624 results in dramatic increase in stalk cell number and defects in germaria and early egg chambers.

(Woichansky et al., 2016)

Expression of Rab5^GD10492 under the control of Scer\GAL4^hh.PU (in combination with a Gal80[ts] transgene to restrict expression to larval stages) results in the posterior compartment of the wing disc displaying a similar rate of growth as compared to controls, but these continue to grow indefinitely, as long as the larva is alive, and exhibit an increase in the number of apoptotic cells, in contrast to controls. These larvae exhibit a significant developmental delay.

Expression of Rab5^GD10492 under the control of Scer\GAL4^salm.EPv (in combination with a Gal80[ts] transgene to restrict expression to the third instar larval stage) in the wing pouch results in defects in endocytosis, increased size of the mutant domain as compared to controls, the mutant domain continues to grow as long as the larvae are alive, and exhibits an increase in the number of apoptotic cells and an increase in cell proliferation, as compared to controls.

Clones expressing Rab5^GD10492 under the control of Scer\GAL4^hh.PU in the posterior compartment of the wing disc are able to grow normally up to 48h after clone initiation, but the cells then enter apoptosis (largely at the clone borders) and a majority of clones disappear or are reduced to a very small size.

(Ballesteros-Arias et al., 2014)

~70% of males expressing Rab5^GD10492 under the control of Scer\GAL4^Abd-B-LDN show incomplete genitalia rotation.

(Lee et al., 2011)

Adults expressing Rab5^GD10492 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Rab5^GD10492 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in partial lethality or complete lethality, depending on the particular P{GD10492} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression (with Dcr-2^Scer\UAS.cDa) via Scer\GAL4^pnr-MD237 results in a severe cleft thorax.

(Thomas et al., 2009)

Clones expressing Rab5^GD10492 do not affect apical cell area.

(Warner and Longmore, 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10492

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab5^GD10492, Scer\GAL4^salm.EPv has neoplasia | third instar larval stage phenotype, suppressible by Dronc^GD12376, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Rab5^GD10492, Scer\GAL4^salm.EPv has increased cell death | third instar larval stage phenotype, suppressible by Dronc^GD12376, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Rab5^GD10492, Scer\GAL4^salm.EPv has neoplasia | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Rab5^GD10492 has increased occurrence of cell division | third instar larval stage phenotype, suppressible by Scer\GAL4^salm.EPv/Dronc^GD12376

(Ballesteros-Arias et al., 2014)

NOT suppressed by

Statement

Reference

Rab5^GD10492, Scer\GAL4^hh.PU has increased cell death | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Ballesteros-Arias et al., 2014)

Suppressor of

Statement

Reference

Rab5^GD10492/Scer\GAL4^Toll-6-D42 is a suppressor | partially of abnormal neurophysiology | dominant | third instar larval stage phenotype of Vps35^MH20

(Inoshita et al., 2017)

Other

Statement

Reference

Rab5^GD10492, Ras85D^V12.UAS, Scer\GAL4^hh.PU has neoplasia | somatic clone | larval stage phenotype

(Ballesteros-Arias et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rab5^GD10492, Scer\GAL4^salm.EPv has wing pouch | third instar larval stage phenotype, suppressible by Dronc^GD12376, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Rab5^GD10492, Scer\GAL4^salm.EPv has wing pouch | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

NOT suppressed by

Statement

Reference

Rab5^GD10492, Scer\GAL4^hh.PU has wing disc posterior compartment | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Ballesteros-Arias et al., 2014)

Suppressor of

Statement

Reference

Rab5^GD10492/Scer\GAL4^Act5C.PI is a suppressor of adherens junction | somatic clone phenotype of Rho1^72O

(Warner and Longmore, 2009)

Rab5^GD10492/Scer\GAL4^Act5C.PI is a suppressor of adherens junction | somatic clone phenotype of Rho1^72F

(Warner and Longmore, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Rab5^GD10492 and pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

(Gerlach et al., 2018)

The expression of Rab5^GD10492 under the control of Scer\GAL4^Toll-6-D42 partially suppresses the decreased excitatory junction potential amplitude under high stimulation conditions observed at the third instar larval neuromuscular junction of Vps35^MH20 heterozygotes.

(Inoshita et al., 2017)

Co-expression of Ras85D^V12.Scer\UAS fails to rescue the increased apoptosis observed in clones expressing Rab5^GD10492 under the control of Scer\GAL4^hh.PU in the posterior compartment of the wing disc, however if there are many of these clones in the wing disc, they are able to form invasive tumors despite containing many cells in apoptosis.

Co-expression of Dronc^GD12376 results in significant suppression of the increased apoptosis and increased proliferation of the mutant domain, preventing tumorigenesis in flies expressing Rab5^GD10492 under the control of Scer\GAL4^salm.EPv (in combination with a Gal80[ts] transgene to restrict expression to the third instar larval stage) in the wing pouch.

Co-expression of puc^Scer\UAS.cMa results in suppression of the tumorigenesis observed in the mutant domain in flies expressing Rab5^GD10492 under the control of Scer\GAL4^salm.EPv (in combination with a Gal80[ts] transgene to restrict expression to the third instar larval stage) in the wing pouch.

(Ballesteros-Arias et al., 2014)

Expression of Rab5^GD10492 in Rho1^72F or Rho1^72O clones suppresses the adherens junction defect seen between two Rho1^72F or Rho1^72O cells, although decreased apical tension is not affected.

(Warner and Longmore, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v34094

w¹¹¹⁸ P{GD10492}v34094

v34096

w¹¹¹⁸; P{GD10492}v34096

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10492

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab5^GD10492

Rab5^KD

(Ballesteros-Arias et al., 2014)

Rab5^RNAi

(Zhao et al., 2022)

rab5^RNAi

(Scholz et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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