FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\TBPHGD6943
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General Information
Symbol
Dmel\TBPHGD6943
Species
D. melanogaster
Name
FlyBase ID
FBal0208690
Feature type
allele
Associated gene
Dmel\TBPH
Associated Insertion(s)
Carried in Construct
P{GD6943}
Also Known As
UAS-TBPH RNAi, TBPHRNAi, TBPH-RNAi, UAS-TBPH-RNAi, UAS-dTDP-43 RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD6943}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD6943
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal learning | adult stage, with Scer\GAL4ey-OK107

      (Lin et al., 2011)

      abnormal locomotor behavior, with Dcr-2UAS.cDa, Scer\GAL4repo.PU, TBPHΔ23

      (Romano et al., 2015)

      abnormal locomotor behavior, with Scer\GAL4elav.PLu

      (Feiguin et al., 2009)

      abnormal locomotor behavior, with Scer\GAL4elav.PLu, TBPHΔ23

      (Feiguin et al., 2009)

      abnormal locomotor behavior, with Scer\GAL4insc-Mz1407

      (Feiguin et al., 2009)

      abnormal locomotor behavior | adult stage, with Scer\GAL4elav.PU, TBPHΔ23

      (Romano et al., 2014)

      abnormal locomotor behavior | adult stage, with Scer\GAL4Toll-6-D42

      (Estes et al., 2011)

      abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4Tub.PU, TBPHΔ23

      (Romano et al., 2014)

      abnormal locomotor behavior | larval stage, with Scer\GAL4elav.PU

      (Lin et al., 2011)

      abnormal locomotor behavior | third instar larval stage, with Scer\GAL4Toll-6-D42

      (Coyne et al., 2015, Estes et al., 2011)

      abnormal neuroanatomy, with Dcr-2UAS.cDa, Scer\GAL4repo.PU, TBPHΔ23

      (Romano et al., 2015)

      abnormal neuroanatomy, with Scer\GAL4ey-OK107

      (Li et al., 2010)

      abnormal neuroanatomy | adult stage, with Scer\GAL4elav.PU, TBPHΔ23

      (Romano et al., 2014)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4221, TBPHQ367X

      (Lu et al., 2009)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4elav.PU

      (Lin et al., 2011)

      abnormal neurophysiology, with Dcr-2UAS.cDa, Scer\GAL4repo.PU, TBPHΔ23

      (Romano et al., 2015)

      increased cell death, with Scer\GAL4ey-OK107

      (Li et al., 2010)

      lethal, with Scer\GAL4Tub.PU

      (Lu et al., 2009)

      short lived, with Dcr-2UAS.cDa, Scer\GAL4repo.PU, TBPHΔ23

      (Romano et al., 2015)

      short lived, with Scer\GAL4elav.PU, TBPHΔ23

      (Romano et al., 2014)

      short lived, with Scer\GAL4Toll-6-D42

      (Estes et al., 2011)

      short lived, with Scer\GAL4Tub.PU, TBPHΔ23

      (Romano et al., 2014)

      viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu

      (Neely et al., 2010)

      viable, with Scer\GAL4ey-OK107

      (Lin et al., 2011)

      viable, with Scer\GAL4Mef2.PR

      (Schnorrer et al., 2010)

      viable, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      viable, with Scer\GAL4tin.CΔ4

      (Neely et al., 2010)

      visible | adult stage

      (Li et al., 2013)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of TBPHGD6943 under the control of Scer\GAL4GMR.PS leads to no obvious eye phenotype, as compared to controls.

      (Ishiguro et al., 2017)

      Expression of TBPHGD6943 RNAi under the control of Scer\GAL4Toll-6-D42 lengthens the time it takes for third instar larvae to roll over in a turning assay.

      (Coyne et al., 2015)

      Expression of TBPHGD6943 under the control of Scer\GAL4repo.PU (in combination with Dcr-2 expression for efficient RNAi) in a TBPHΔ23 heterozygous background results in strong motility defects, as well as consistent reduction of life span, compared with wild-type

      (Romano et al., 2015)

      Flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PU in a TBPHΔ23/+ mutant background exhibit significant climbing defects, a severe reduction in life span, and degeneration of the neuropil, as compared to controls.

      (Romano et al., 2014)

      Flies expressing TBPHGD6943 in a TBPHΔ23/+ mutant background under the control of Scer\GAL4tub.PU, and restricted to the adult stage using Scer\GAL80ts.αTub84B, exhibit progressive climbing defects and reduced lifespan.

      Larvae expressing TBPHGD6943 under the control of Scer\GAL4tub.PU from the third instar larval stage onward (using Scer\GAL80ts.αTub84B) in a TBPHΔ23/+ background) exhibit locomotor defects from 24 hours after the temperature shift. These alterations in fly motility are not associated with anatomical modifications in the number of synaptic boutons or motoneuron terminal branches at the NMJ, except for the presence of subtle alterations in the morphology of the synaptic boutons compared to controls.

      (Romano et al., 2014)

      Expression of TBPHGD6943 under the control of Scer\GAL4Act.PU results in a subset of adults possessing supernumerary scutellar bristles or supernumerary anterior postalar bristles.

      (Li et al., 2013)

      Expression of TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 results in locomotor defects in third instar larvae. Crawling larvae rolled ventral side up take longer to turn back to dorsal side up.

      Adults expressing TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 exhibit a progressive decline in their ability to climb at 18[o]C, and exhibit a decrease in lifespan, as compared to controls.

      (Estes et al., 2011)

      When TBPHGD6943 is expressed pan-neuronally under the control of Scer\GAL4elav.PU the synaptic boutons on muscles 6 and 7 in third instar larvae appear deformed and are irregularly spaced along the terminal axons with several fused or elongated silhouettes and clear loss of their characteristic round-smooth shape.

      (Godena et al., 2011)

      Expression of TBPHGD6943 under the control of Scer\GAL4ey-OK107 does not affect the morphology of the mushroom body lobes. The flies show a small (10%) but significant reduction in performance in an odour avoidance learning assay compared to controls.

      Expression of TBPHGD6943 under the control of either Scer\GAL4Mhc.PU or Scer\GAL4D42 does not result in locomotor defects.

      Expression of TBPHGD6943 under the control of Scer\GAL4elav.PU results in locomotor defects in larvae. The number of synaptic boutons normalised to the muscle area is increased at the neuromuscular junction in these animals.

      (Lin et al., 2011)

      No obvious eye defect is found in flies expressing TBPHGD6943 under the control of Scer\GAL4GMR.PF.

      Expression of TBPHGD6943 in the mushroom body (MB) under the control of Scer\GAL4ey-OK107 results in axonal loss and neuronal death. No obvious abnormal axonal varicosities are detected in TBPHGD6943-expressing MBs.

      The locomotive ability of larvae expressing TBPHGD6943 under the control of Scer\GAL4VGlut-OK371 is normal.

      (Li et al., 2010)

      Adults expressing TBPHGD6943 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression of TBPHGD6943 in the developing eye under the control of Scer\GAL4GMR.PF does not affect eye development.

      (Ritson et al., 2010)

      Adults expressing TBPHGD6943 under the control of either Scer\GAL41407 or Scer\GAL4elav.PLu show defects in climbing ability.

      Flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PLu in a TBPHΔ23/+ background show defects in both walking and climbing assays.

      (Feiguin et al., 2009)

      Expression of TBPHGD6943 under the control of Scer\GAL4221 in a TBPHQ367X/+ background results in a significant decrease in dendritic branching in the ddaE and ddaF neurons of third instar larvae.

      (Lu et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The longer time it takes for third instar larvae expressing TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 to roll over in a turning assay compared to wild-type controls is lengthened further by co-expression of Fmr1GD1288 RNAi.

      (Coyne et al., 2015)

      Knockdown of TBPH, through expression of TBPHGD6943, enhances the eye phenotype seen in flies expressing Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU.

      (Galloway et al., 2014)

      Co-expression of Hrb98DEJF01249 enhances the climbing defects, shortened life span and neuropil degeneration seen in TBPHΔ23/+ flies expressing TBPHGD6943 under the control of Scer\GAL4elav.PU.

      Expression of TBPHGD6943 under the control of Scer\GAL4elav.PU enhances the climbing defects, shortened life span and neuropil degeneration seen in TBPHΔ23/+ flies expressing Hrb98DEJF01249 under the control of Scer\GAL4elav.PU.

      (Romano et al., 2014)

      Expression of Syx1AScer\UAS.cBa significantly suppresses the climbing defects seen in adult hypomorphic TBPH mutants (generated by expressing TBPHGD6943 in adult flies under the control of Scer\GAL4tub.PU and Scer\GAL80ts.αTub84B, in a TBPHΔ23/+ background).

      (Romano et al., 2014)

      Expression of TBPHGD6943 in the developing eye dominantly suppresses the eye degeneration phenotype found upon expression of TER94R152H.Scer\UAS.R (with both transgenes driven by Scer\GAL4GMR.PF). This is concurrent with a significant reduction in the blinded phenotypic severity score.

      (Ritson et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      The co-expression of TBPHGD6943 enhances the severity of the eye defects resulting from the expression of Zzzz\UGGAAW.Scer\UAS under the control of Scer\GAL4GMR.PS (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities); the severe eye defects resulting from this co-expression are fully or partially suppressed by the additional co-expression of Hsap\FUSScer\UAS.cIa or Hsap\HNRNPA2B1Scer\UAS.cKa, respectively.

      The co-expression of TBPHGD6943 and Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS leads to lethality, which is rescued by the additional co-expression of either Hsap\FUSScer\UAS.cIa or Hsap\HNRNPA2B1Scer\UAS.cKa; the rescued adults exhibit significantly less severe eye phenotypes, as compared to adults only expressing Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS.

      (Ishiguro et al., 2017)

      Expression of TBPHGD6943 enhances the third instar larval locomotor defects seen when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed in motor neurons under the control of Scer\GAL4D42.

      Expression of TBPHGD6943 enhances the third instar larval locomotor defects seen when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed in motor neurons under the control of Scer\GAL4D42.

      (Estes et al., 2011)

      Co-expression of TBPHGD6943 with Hsap\TARDBPScer\UAS.T:Ivir\haemagglutinin,T:Disc\RFP under the control of Scer\GAL4VGlut-OK371 results in improved larval movement compared to the Hsap\TARDBPScer\UAS.T:Ivir\haemagglutinin,T:Disc\RFP-expressing parental line.

      (Li et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TBPHGD6943
      TBPHRNAi
      (Cragnaz et al., 2015, Romano et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (30)