FlyBase Allele Report: Dmel\rod[GD8768]

General Information

Symbol

Dmel\rod^GD8768

Species

D. melanogaster

Name

FlyBase ID

FBal0210484

Feature type

allele

Associated gene

Dmel\rod

Associated Insertion(s)

Carried in Construct

P{GD8768}

Also Known As

UAS-rod^RNAi, rod-i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8768}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8768

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with BacA\p35^UAS.cHa

(Muzzopappa et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | larval stage, with Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

increased cell death | larval stage, with Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing disc posterior compartment, with Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Detailed Description

Statement

Reference

The expression of rod^GD8768 under the control of Scer\GAL4^e22c leads to lagging chromosomes during mitosis, X chromosome aneuploidy, increased DNA damage, increased apoptosis and basal delamination in the wing disc posterior compartment of both females and males; loss of the X chromosome was detected in females, but never in males.

(Clemente-Ruiz et al., 2016)

Larvae expressing rod^GD8768 under the control of Scer\GAL4^Hml.PG show normal rates of encapsulation of eggs when parasitised by the avirulent L. boulardi wasp strain G486.

(Howell et al., 2012)

Adults expressing rod^GD8768 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8768

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^e22c, rod^GD8768 has abnormal DNA repair | larval stage phenotype, enhanceable | male by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, enhanceable | female by DNAlig4^KK101734/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has abnormal DNA repair | larval stage phenotype, enhanceable by okr^KK107487, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has abnormal DNA repair | larval stage phenotype, enhanceable by DNAlig4^KK101734, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, enhanceable | female by msl-1^RNAi.UAS.cUa/Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, enhanceable | female by Sxl^HMS00609/Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, enhanceable | female by grp^KK108548/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, enhanceable | female by okr^KK107487/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT Enhanced by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has abnormal DNA repair | larval stage phenotype, non-enhanceable by lic^RNAi.UAS.cUa, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768 has neoplasia | larval stage phenotype, suppressible by wg^GD5007/Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, suppressible | partially by Cat^UAS.cAa/Sod2^UAS.cMa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has increased cell death | larval stage phenotype, suppressible by puc^UAS.cMa/lic^RNAi.UAS.cUa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has neoplasia | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has neoplasia | larval stage phenotype, non-suppressible by egr¹/egr¹

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has neoplasia | larval stage phenotype, non-suppressible by grnd^KK109939, Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has neoplasia | larval stage phenotype, non-suppressible by grnd^MI05292/grnd^MI05292

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has neoplasia | larval stage phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768 has increased occurrence of cell division | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has increased cell number | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has abnormal DNA repair | larval stage phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, okr^KK107487, rod^GD8768 has abnormal size | larval stage phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, DNAlig4^KK101734, Scer\GAL4^ap-md544, rod^GD8768 has abnormal size | larval stage phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Cat^UAS.cAa, Scer\GAL4^ap-md544, Sod2^UAS.cMa, rod^GD8768 has abnormal size | larval stage phenotype

(Clemente-Ruiz et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | male by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | female by DNAlig4^KK101734/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc dorsal compartment phenotype, enhanceable by okr^KK107487, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc dorsal compartment phenotype, enhanceable by DNAlig4^KK101734, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | female by msl-1^RNAi.UAS.cUa/Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | female by Sxl^HMS00609/Scer\GAL4^e22c

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | female by grp^KK108548/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, enhanceable | female by okr^KK107487/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT Enhanced by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc dorsal compartment phenotype, non-enhanceable by lic^RNAi.UAS.cUa, Scer\GAL4^ap-md544

(Clemente-Ruiz et al., 2016)

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768 has wing disc phenotype, suppressible by wg^GD5007/Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, suppressible | partially by Cat^UAS.cAa/Sod2^UAS.cMa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Scer\GAL4^e22c, rod^GD8768 has wing disc posterior compartment phenotype, suppressible by puc^UAS.cMa/lic^RNAi.UAS.cUa/Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc phenotype, non-suppressible by egr^RNAi.UAS, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has wing disc phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has wing disc phenotype, non-suppressible by egr¹/egr¹

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has wing disc phenotype, non-suppressible by grnd^KK109939, Scer\GAL4^hh.PU

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has wing disc phenotype, non-suppressible by grnd^MI05292/grnd^MI05292

(Muzzopappa et al., 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768 has wing disc phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has myoblast | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, rod^GD8768 has wing disc phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, rod^GD8768 has wing disc dorsal compartment phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, okr^KK107487, rod^GD8768 has wing disc posterior compartment phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, DNAlig4^KK101734, Scer\GAL4^ap-md544, rod^GD8768 has wing disc posterior compartment phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Cat^UAS.cAa, Scer\GAL4^ap-md544, Sod2^UAS.cMa, rod^GD8768 has wing disc posterior compartment phenotype

(Clemente-Ruiz et al., 2016)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, Sod2^UAS.cMa, rod^GD8768 has wing disc posterior compartment phenotype

(Clemente-Ruiz et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of rod^GD8768 and BacA\p35^UAS.cHa under the control of Scer\GAL4^ap-md544 induces wing disc neoplasia, with amplification of the resident myoblast population. Myoblast ablation (by expressing rpr^lexAop.cSa under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth.

The co-expression of rod^GD8768 and BacA\p35^UAS.cHa under the control of Scer\GAL4^hh.PU induces wing disc tumor-like growth. Myoblast ablation (by expressing rpr^lexAop.cSa under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth.

The co-expression of rod^GD8768 and BacA\p35^UAS.cHa under the control of Scer\GAL4^en-e16E induces wing disc tumor-like growth, with increased mitotic index.

(Muzzopappa et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v19152

w¹¹¹⁸; P{GD8768}v19152

Notes on Origin

Discoverer