FlyBase Allele Report: Dmel\lncRNA:flam[BG02658]

General Information

Symbol

Dmel\lncRNA:flam^BG02658

Species

D. melanogaster

Name

FlyBase ID

FBal0212109

Feature type

allele

Associated gene

Dmel\lncRNA:flam

Associated Insertion(s)

P{GT1}lncRNA:flam^BG02658

Carried in Construct

Also Known As

flam^BG, flam^BG02658

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GT1}lncRNA:flam^BG02658

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GT1}

gene trap

polyA trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:21,631,511..21,631,511 [-]

(Dennis et al., 2016, Brennecke et al., 2007, Mevel-Ninio et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

modeled by Zzzz\CGG^60.UAS.EGFP

(Tan et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Mevel-Ninio et al., 2007)

female sterile (with Df(1)flam^EY18P)

(Mevel-Ninio et al., 2007)

female sterile (with Df(1)l3)

(Mevel-Ninio et al., 2007)

female sterile (with Df(1)l15)

(Mevel-Ninio et al., 2007)

female sterile (with lncRNA:flam^EY2P)

(Mevel-Ninio et al., 2007)

female sterile (with lncRNA:flam^EY7P)

(Mevel-Ninio et al., 2007)

female sterile (with lncRNA:flam^EY13P)

(Mevel-Ninio et al., 2007)

female sterile (with lncRNA:flam^KG00476)

(Mevel-Ninio et al., 2007)

female sterile (with lncRNA:flam^py+P)

(Mevel-Ninio et al., 2007)

increased cell death (with lncRNA:flam^KG00476)

(Zamparini et al., 2011)

Phenotype Manifest In

dorsal appendage

(Mevel-Ninio et al., 2007)

egg

(Mevel-Ninio et al., 2007)

egg chamber

(Mevel-Ninio et al., 2007)

germline cell

(Mevel-Ninio et al., 2007)

ovariole

(Mevel-Ninio et al., 2007)

ovary

(Mevel-Ninio et al., 2007)

ovary (with lncRNA:flam^KG00476)

(Zamparini et al., 2011)

Detailed Description

Statement

Reference

flam^BG02658 homozygotes do not show significant mortality during the pharate stage.

(Guida et al., 2016)

flam^BG02658 heterozygote flies do not display any defects in the adult eye morphology.

(Tan et al., 2012)

flam^BG02658/flam^KG00476 ovaries show defective germline and soma differentiation phenotypes. Somatic cells also die.

(Zamparini et al., 2011)

Homozygous females do not lay any eggs.

flam^BG02658 flies have a permissive phenotype with respect to gypsy expression (assayed by expression of a P{gypsy-lacZ.p12} reporter construct in the ovaries).

flam^BG02658 flies are unstable for ZAM expression (assayed by expression of a P{ZAM-lacZ.LTR} reporter construct in the ovaries).

24 hour old flam^BG02658 females have ovaries which are very small and contain rudimentary ovarioles within which no differentiation of germ cells has taken place. Ovarioles of 48 hour old mutant females are severely dystrophic, containing two to three completely disorganised egg chambers which contain a reduced number of abnormally shaped nuclei. Mature eggs are rarely formed, and those that do are smaller than normal and appear to lack dorsal-ventral and anterior-posterior polarities. The dorsal appendages are short and abnormally shaped. The follicular epithelium is completely disorganised in the ovaries of 6-day old mutant females.

Mosaic females containing homozygous germline clones develop several completely normal ovarioles and are fertile. The eggs produced by these females give rise to normal adult flies.

(Mevel-Ninio et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

lncRNA:flam^BG02658 has female sterile phenotype, non-suppressible by DIP1^t8

(Mevel-Ninio et al., 2007)

lncRNA:flam^BG02658 has female sterile phenotype, non-suppressible by DIP1^cos7a

(Mevel-Ninio et al., 2007)

Enhancer of

Statement

Reference

lncRNA:flam^BG02658/flam[+] is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

Other

Statement

Reference

ct^A, lncRNA:flam^BG02658 has partially lethal - majority live phenotype

(Guida et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

lncRNA:flam^BG02658/flam[+] is an enhancer of eye | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

flam^BG02658, ct^A double homozygotes present significant mortality during the pharate stage, as compared to controls.

(Guida et al., 2016)

Xenogenetic Interactions

Statement

Reference

The neurodegenerative rough eye phenotype characteristic for adult flies expressing Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU is exacerbated further by combination with a single copy of flam^BG02658 (which is permissive for the gypsy retrotransposon expression).

(Tan et al., 2012)

Complementation and Rescue Data

Complements

lncRNA:flam^679-5b

(Mevel-Ninio et al., 2007)

lncRNA:flam^FM7c

(Mevel-Ninio et al., 2007)

Fails to complement

lncRNA:flam^EY13P

(Mevel-Ninio et al., 2007)

lncRNA:flam^EY7P

(Mevel-Ninio et al., 2007)