FlyBase Allele Report: Zzzz\CGG[60.UAS.EGFP]

General Information

Symbol

Zzzz\CGG^60.UAS.EGFP

Species

a. artificial

Name

FlyBase ID

FBal0280775

Feature type

allele

Associated gene

Zzzz\CGG

Associated Insertion(s)

Carried in Construct

P{UAS-CGG60-EGFP}

Also Known As

UAS-CGG₆₀-EGFP, pUAST-(CGG)₆₀-EGFP, rCGG₆₀

Transgenic product class

trinucleotide_repeat_expansion

(Jin et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Jin et al., 2003)

Mutagen

in vitro construct

(Jin et al., 2003)

Progenitor genotype

Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

Carried in construct

P{UAS-CGG60-EGFP}

Cytology

Description

The 3' end of the Zzzz\CGG repeat array has been contracted, leaving 60 copies, with repeat fragment consisting of the sequence (CGG)[[9]]AGG(CGG)[[9]]AGG(CGG)[[40]].

(Jin et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Zzzz\CGG

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Qurashi et al., 2012)

model of fragile X-associated tremor/ataxia syndrome

CEA

(Bhat et al., 2021, Tan et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of fragile X-associated tremor/ataxia syndrome

is ameliorated by Hrb87F^UAS.cSa

(Tan et al., 2012)

is exacerbated by lncRNA:flam^BG02658

(Tan et al., 2012)

is exacerbated by lncRNA:flam^KG00476

(Tan et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^Act5C.PI

(Jin et al., 2003)

partially lethal - majority live, with Scer\GAL4^elav-C155

(Jin et al., 2003)

some die during larval stage, with Scer\GAL4^Act5C.PI

(Jin et al., 2003)

viable, with Scer\GAL4^elav-C155

(Qurashi et al., 2011)

visible, with Scer\GAL4^GMR.PF

(Jin et al., 2003)

wild-type, with Scer\GAL4^dpp.blk1

(Jin et al., 2003)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jin et al., 2003)

ommatidium, with Scer\GAL4^GMR.PF

(Jin et al., 2003)

pigment cell, with Scer\GAL4^GMR.PF

(Jin et al., 2003)

Detailed Description

Statement

Reference

Scer\GAL4^elav-C155-driven expression of Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP} has no adverse effect on viability.

(Qurashi et al., 2011)

Strong expression of Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF results in rough eyes, loss of pigmentation, and holes are seen in tangential sections of the eye. Moderate expression has no effect on the eye.

Strong expression of Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 results in reduced viability, while moderate expression has no effect.

Strong expression of Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI results in late larval lethality, while moderate expression has no effect.

(Jin et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Rm62[+]/Rm62⁰¹⁰⁸⁶, Scer\GAL4^elav-C155, Zzzz\CGG^60.UAS.EGFP has partially lethal - majority live phenotype

(Qurashi et al., 2011)

Scer\GAL4^elav-C155, Zzzz\CGG^60.UAS.EGFP, sbr¹²/sbr[+] has partially lethal - majority die phenotype

(Qurashi et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Rm62⁰¹⁰⁸⁶ or sbr¹² heterozygotes expressing Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^elav-C155 display reduced viability compared to controls. None of these manipulations alone causes any viability deficits.

(Qurashi et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Avic\GFP^{Fmr1.CGG60.E.Scer\UAS}

Zzzz\CGG^{60.Scer\UAS.T:Avic\GFP-EGFP}

Zzzz\CGG^60.UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

FBal0280529
FBal0157338

References (8)

Report Sections

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