FlyBase Allele Report: Dmel\Marf[RNAi.UTR.UAS]

General Information

Symbol

Dmel\Marf^RNAi.UTR.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258605

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{UAS-Marf.RNAi.UTR}

Key Links

Transgenic product class

RNAi_reagent

(Deng et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Deng et al., 2008)

Mutagen

in vitro construct

(Deng et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Marf.RNAi.UTR}

Cytology

Description

UAS regulatory sequences drive expression of a miRNA targeted to the Marf untranslated region.

(Deng et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Marf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased cell number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased occurrence of cell division | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^sca.PU

(Dubal et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

increased occurrence of cell division | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

increased occurrence of cell division | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^insc.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^pros.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Dubal et al., 2022)

Phenotype Manifest In

intermediate neuronal progenitor | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

larval brain | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

larval brain | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

larval neuron | decreased number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

mitochondrial crista, with Scer\GAL4^Mef2.PR

(Deng et al., 2008)

mitochondrion, with Scer\GAL4^Mef2.PR

(Deng et al., 2008)

mitochondrion | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

mitochondrion | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

muscle cell of A1-7 ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

muscle cell of A1-7 ventral longitudinal muscle 4 | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

type II neuroblast | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

type II neuroblast | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

Detailed Description

Statement

Reference

The expression of Marf^{miRNA.UTR.UAS} under the control of Scer\GAL4^pnt-14-94 results in type II neuroblasts and daughter mature intermediate precursor cells in the larval brain with fragmented mitochondria. Young neurons are decreased in number. The mature intermediate precursor cells show increased mitotic index, while daughter ganglion mother cells show decreased mitotic index. Type II neuroblast lineages show increased proliferation (increased S-phase) compared to controls.

The expression of Marf^{miRNA.UTR.UAS} under the control of Scer\GAL4^wor.PU results in larval brain type II neuroblast lineages to show increased proliferation (increased S-phase) compared to controls.

(Dubal et al., 2022)

Expression of Marf^{miRNA.UTR.Scer\UAS} under the control of Scer\GAL4^Mef2.PR results in mitochondrial fragmentation (they are smaller and rounder than normal) in the indirect flight muscles. The mitochondria also have irregular cristae.

(Deng et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has decreased cell number | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has increased occurrence of cell division | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has decreased occurrence of cell division | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^wor.PU has increased occurrence of cell division | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^wor.PU

(Dubal et al., 2022)

Suppressor of

Statement

Reference

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a suppressor | partially of increased cell number | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS/Scer\GAL4^Mef2.PR is a suppressor of visible phenotype of Pink1⁵

(Deng et al., 2008)

Other

Statement

Reference

Drp1^SD.UASp, Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has increased occurrence of cell division | larval stage phenotype

(Dubal et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has larval brain phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has larval neuron | larval stage | decreased number phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has intermediate neuronal progenitor | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has ganglion mother cell | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^wor.PU has larval brain | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^wor.PU

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^wor.PU has type II neuroblast | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^wor.PU

(Dubal et al., 2022)

Suppressor of

Statement

Reference

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a suppressor | partially of larval brain phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a suppressor | partially of type II neuroblast | increased number | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS/Scer\GAL4^elav.PU is a suppressor of mitochondrion phenotype of Drp1²/Drp1¹

(Rival et al., 2011)

Marf^RNAi.UTR.UAS/Scer\GAL4^how-24B is a suppressor of mitochondrion phenotype of park²⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^how-24B is a suppressor of mitochondrial crista phenotype of park²⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^how-24B is a suppressor of indirect flight muscle cell phenotype of park²⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^Mef2.PR is a suppressor of mitochondrion phenotype of Pink1⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^Mef2.PR is a suppressor of mitochondrial crista phenotype of Pink1⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^Mef2.PR is a suppressor of wing phenotype of Pink1⁵

(Deng et al., 2008)

Marf^RNAi.UTR.UAS/Scer\GAL4^Mef2.PR is a suppressor of indirect flight muscle cell phenotype of Pink1⁵

(Deng et al., 2008)

NOT Suppressor of

Statement

Reference

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a non-suppressor of larval brain phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a non-suppressor of type II neuroblast | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a non-suppressor of intermediate neuronal progenitor | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 is a non-suppressor of mitochondrion | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Other

Statement

Reference

Drp1^SD.UASp, Marf^RNAi.UTR.UAS, Scer\GAL4^pnt-14-94 has type II neuroblast | larval stage phenotype

(Dubal et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Drp1^SD.UASp and Marf^{miRNA.UTR.UAS} under the control of Scer\GAL4^pnt-14-94 results in type II neuroblasts in the larval brain showing decreased mitotic index.

(Dubal et al., 2022)

Expression of Marf^{miRNA.UTR.Scer\UAS} under the control of Scer\GAL4^elav.PU is sufficient to restore a normal filamentous mitochondrial network in Drp1¹/Drp1² flies.

Expression of Marf^{miRNA.UTR.Scer\UAS} under the control of Scer\GAL4^elav.PU does not rescue the abnormal mitochondrial morphology of Df(3L)Pmi-PGRP-LD flies.

(Rival et al., 2011)

Expression of Marf^{miRNA.UTR.Scer\UAS} under the control of Scer\GAL4^Mef2.PR suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink1⁵ mutants. The defects in wing posture and indirect flight muscle degeneration phenotypes are also suppressed.

Expression of Marf^{miRNA.UTR.Scer\UAS} under the control of Scer\GAL4^how-24B suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of park²⁵ mutants. The indirect flight muscle degeneration phenotype is also suppressed.

(Deng et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Marf^RNAi.UTR.UAS

Marf^{miRNA.UTR.Scer\UAS}

Marf^{miRNA.UTR.UAS}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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