FlyBase Allele Report: Scer\GAL4[0273-G4]

General Information

Symbol

Scer\GAL4^0273-G4

Species

S. cerevisiae

Name

FlyBase ID

FBal0283998

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

PBac{IT.GAL4}0273-G4

Carried in Construct

Also Known As

0273-GAL4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{IT.GAL4}0273-G4

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{IT.GAL4}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult dopaminergic PAM neuron | subset

(Huetteroth et al., 2015)

medial lobe of adult mushroom body

(Huetteroth et al., 2015)

adult dopaminergic PAM neuron

(Burke et al., 2012)

Additional Information

Statement

Reference

ScerGAL4^0273-G4 labels ~130 dopaminergic PAM neurons that innervate the medial lobe of the adult mushroom body.

(Huetteroth et al., 2015)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with shi^1.UAS

(Lin et al., 2014)

abnormal behavior, with shi^{ts1.IVS.p10.20xUAS}

(Lin et al., 2014)

abnormal learning | temperature conditional, with shi^1.UAS

(Lin et al., 2014)

abnormal learning | temperature conditional, with shi^{ts1.IVS.p10.20xUAS}

(Lin et al., 2014)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Ecol\lexA^{ChAT-MI04508-TlexA:QFAD.0}, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Ecol\lexA^GMR61H03, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Ecol\lexA^p65.Trhn, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR58E02

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^Gr66a.PD

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL80^ple.PK

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | conditional, with Gthe\ACR1^UAS.d.EYFP

(Stern et al., 2019)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Huetteroth et al., 2015)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Huetteroth et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 624 nm (red) light) of Scer\GAL4^0273-G4 cells significantly increases preference for areas with red light and navigation to areas previously associated with illumination, compared to controls; restricting activation to smaller subsets of Scer\GAL4^0273-G4 cells can reduce the preference for red light (using Ecol\lexA^{ChAT-MI04508-TlexA:QFAD.0}, but not Ecol\lexA^GMR61H03, to drive Scer\GAL80^{IVS.WPRE.8xlexAop}) and/or reduce the navigation toward areas previously associated with illumination (using Ecol\lexA^GMR61H03, Ecol\lexA^{ChAT-MI04508-TlexA:QFAD.0} or Ecol\lexA^p65.Trh to drive Scer\GAL80^{IVS.WPRE.8xlexAop} or using Scer\GAL80^ple.PK, but not Scer\GAL4^GMR58E02), relative to activation of all Scer\GAL4^0273-G4 cells; activation of the combined set of Scer\GAL4^0273-G4 cells and Scer\GAL4^Gr66a.PD cells also increases preference for areas with red light, compared to controls; activation with 527 nm (green) light of Scer\GAL4^0273-G4 cells increases preference for areas with green light, compared to controls.

Optogenetic inhibition (using Gthe\ACR1^UAS.d.EYFP with 527 nm (green) light) of Scer\GAL4^0273-G4 cells results in avoidance of areas with green light, unlike controls.

(Stern et al., 2019)

Blocking the output (using shi^1.UAS at 33[o]C) of Scer\GAL4^0273-G4 neurons shortly before and during training significantly impairs long-term olfactory memory in starved flies trained with sucrose, compared to controls.

Acute thermogenetic activation (using TrpA1^UAS.(B).cKa at 33[o]C) of Scer\GAL4^0273-G4 neurons results in a significant long-term preference for a paired odor (artificial memory) in starved or fed flies, compared to controls; even when flies are fed, this memory persists for at least 7 days.

(Huetteroth et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247}

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4, norpA^P24 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^GMR38G08

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Adcy1^rut-1

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Pde4^dnc-1

(Stern et al., 2019)

Suppressed by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4, norpA^P24 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^GMR13F02

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^GMR38G08

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by norpA^P24

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Dop1R1^f02676

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Dop2R^f06521

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by ple^HMC06137, Scer\GAL4^0273-G4

(Stern et al., 2019)

NOT suppressed by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Adcy1^rut-1

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Pde4^dnc-1

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^{VP16.Mef2.247}

(Stern et al., 2019)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4, norpA^P24 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Gthe\ACR1^{lexAop.d.EYFP}/Ecol\lexA^GMR38G08

(Stern et al., 2019)

Suppressor of

Statement

Reference

ple^HMC06137, Scer\GAL4^0273-G4 is a suppressor | partially of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

norpA³⁶ partially suppresses the navigation of adults with optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 624 nm (red) light) of Scer\GAL4^0273-G4 cells to areas associated with illumination only when the assay is performed in an arena lacking tactile features.

(Stern et al., 2019)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^0273-G4

Scer\GAL4⁰²⁷³

Name Synonyms

Secondary FlyBase IDs

References (11)

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