FlyBase Allele Report: Scer\GAL4[DBD.R18D09]

General Information

Symbol

Scer\GAL4^DBD.R18D09

Species

S. cerevisiae

Name

FlyBase ID

FBal0304435

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R18D09-GAL4.DBD}

Also Known As

R18D09-ZpGdbd, R18D09

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R18D09-GAL4.DBD}

Cytology

Description

The R18D09 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR18D09

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body output neuron j1

Comment: when combined with P{VT057469-p65.AD} (combination referred to as 'SS01973')

(Truman et al., 2023, Saumweber et al., 2018)

adult stage

dopaminergic PAM neuron 1

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 11 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 13 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 14 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 2 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 4 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 5 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 6 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 7 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 8 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 12

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Yamagata et al., 2015, Aso et al., 2014)

LNd neuron | subset

Comment: when combined with P{R12G04-p65.AD} (combination referred to as 'MB122B')

(Liang et al., 2017)

adult Pdf negative s-LNv neuron

Comment: when combined with P{R12G04-p65.AD} (combination referred to as 'MB122B')

(Liang et al., 2017)

s-LNv Pdf neuron

Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'SS00681')

(Liang et al., 2017)

dopaminergic PAM neuron 4

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Aso et al., 2014)

dopaminergic PAM neuron 8

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB040B')

(Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R58E02

(Lewis et al., 2015)

abnormal cell number | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal cell number | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal feeding behavior | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal locomotor rhythm | adult stage, with Hsap\KCNJ2^UAS.EGFP, Hsap\RELA^AD.R12G04

(Bulthuis et al., 2019)

abnormal locomotor rhythm | adult stage, with Hsap\RELA^AD.R12G04, kcc^KK107965

(Eick et al., 2022)

abnormal locomotor rhythm | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal locomotor rhythm | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

decreased rate of movement | adult stage, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

decreased rate of movement | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

decreased rate of movement | adult stage, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

Phenotype Manifest In

adult brain, with Hsap\RELA^AD.R12G04, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

adult brain, with Hsap\RELA^AD.R65B09, Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

Detailed Description

Statement

Reference

Elimination of molecular clock function (using cyc^{Δ.UAS.Tag:MYC}) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.R12G04 in adults does not affect the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.R12G04 significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when inhibition is restricted to the adult stage using a Gal80[ts] transgene.

(Bulthuis et al., 2019)

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.VT057469 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, innate odor (n-amylacetate) preference or innate fructose preference, compared to controls.

(Saumweber et al., 2018)

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R18D09 and Hsap\RELA^AD.R58E02 in adults results in a significant attraction to the illuminated area, no attraction is observed in controls.

(Lewis et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions