Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\r-l using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\r-l in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: r-l CG3593
Full sequence and embryonic transcript of r-l have been determined and the deduced UMP synthase structure compared with other protein sequences; major structural features among these proteins are revealed.
Molecular cloning of r-l has been performed to permit analysis of its regulation relative to other pyrimidine pathway genes.
Encodes a polypeptide with the enzyme activities that catalyze the fifth and sixth steps of de novo pyrimidine synthesis: orotate phosphoribosyl transferase (OPRT) and orotidylate decarboxylase (ODC) respectively. Thought to exist as a homodimer (FBrf0032871). The first three enzyme activities encoded by r and the fourth by Dhod.
OPRT and ODC activities drastically reduced except that OPRT moderately reduced in r-lK7 and ODC above normal in r-lK6. All mutants accumulate orotic acid, the substrate of ODC. Flies hemizygous or homozygous for mutants at this locus resemble r in that they have obliquely truncated wings with deranged marginal hairs and virtual female sterility; they differ from r in having faintly mottled eyes and severely impaired viability (viability of hypomorphs moderately reduced). r is epistatic to r-l in that r; r-l flies display the r rather than the r-l phenotype, suggesting that mottled eyes and reduced viability are the consequence of orotic-acid accumulation rather than pyrimidine deficiency.