FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\fs(1)N
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General Information
Symbol
Dmel\fs(1)N
Species
D. melanogaster
Name
female sterile (1) Nasrat
Annotation Symbol
CG11411
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004650
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
female sterile (1) Nasrat (fs(1)N) encodes encodes a protein that is required, along with the products of fs(1)M3 and clos, for their accumulation at the vitelline membrane and function in vitelline membrane integrity and Torso receptor activation. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

fs(1)Nasrat, Nasrat, fs(1)Nas, fs(1)M6, fs(1)K1540

Key Links
Genomic Location
Cytogenetic map
1F3-1F3
Sequence location
Recombination map
1-0.1
RefSeq locus
NC_004354 REGION:1322513..1329438
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables heparin binding
inferred from biological aspect of ancestor with PANTHER:PTN001116154
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in chorion-containing eggshell formation
inferred from expression pattern
(Jimenez et al., 2002)
inferred from mutant phenotype
(Degelmann et al., 1990)
involved_in terminal region determination
inferred from mutant phenotype
(Stevens et al., 2003, Jimenez et al., 2002, Furriols et al., 1998, Degelmann et al., 1990)
involved_in torso signaling pathway
inferred from mutant phenotype
(Jimenez et al., 2002, Furriols et al., 1998)
involved_in vitelline membrane formation involved in chorion-containing eggshell formation
inferred from mutant phenotype
(Cernilogar et al., 2001)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in external side of plasma membrane
inferred from direct assay
(Jimenez et al., 2002)
located_in vitelline envelope
inferred from direct assay
(Ventura et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cell surface
inferred from biological aspect of ancestor with PANTHER:PTN001116154
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
female sterile (1) Nasrat (fs(1)N) encodes encodes a protein that is required, along with the products of fs(1)M3 and clos, for their accumulation at the vitelline membrane and function in vitelline membrane integrity and Torso receptor activation. [Date last reviewed: 2019-09-26]
Pathway (FlyBase)
TORSO SIGNALING PATHWAY CORE COMPONENTS -
The formation of Drosophila embryonic termini is controlled by the localized activation of Torso (tor) receptor tyrosine kinase. The Torso signaling pathway acts via the canonical Ras/Raf/MAP kinase cascade. (Adapted from FBrf0157176.)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
fs(1)N: female sterile (1) of Nasrat
Maternal lethal. Developmental study of fs(1)N1 by Counce and Ede. Eggs of fs(1)N/fs(1)N females will not support development of normal embryos. About half the eggs contain little or no yolk; development may or may not begin in such eggs but never progresses beyond a highly abnormal cleavage. In eggs containing more yolk, major effect is on synchrony of cleavage and blastoderm mitoses. Twenty percent of these embryos cease development before blastoderm formation. The remainder have abnormal blastoderms and aberrant gastrulation. Final pattern of damage determined by degree of abnormality of earlier stages, but some embryos show larval differentiation. A few of the least abnormal embryos may emerge but never move about or feed. Formation of polar granules abnormal. RK3.
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\fs(1)N for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O76904)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
fs(1)N-RA
FBtr0070207
NM_130545
6581
2118
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
fs(1)N-PA
FBpp0070200
238.8
2118
5.00
O76904
NP_569901
AAF45603
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)N using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.18

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in external side of plasma membrane
inferred from direct assay
(Jimenez et al., 2002)
located_in vitelline envelope
inferred from direct assay
(Ventura et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\fs(1)N in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 22 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of fs(1)N
Transgenic constructs containing regulatory region of fs(1)N
Aberrations (Deficiencies and Duplications) ( 17 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | maternal effect
lethal | maternal effect | heat sensitive
lethal - all die during embryonic stage, with fs(1)N14
lethal - all die during embryonic stage, with fs(1)NΔ6
lethal - all die during embryonic stage | maternal effect
lethal - all die during embryonic stage | maternal effect (with fs(1)N12)
lethal - all die during embryonic stage | maternal effect (with fs(1)N14)
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Sterility
Allele
female sterile
female sterile | recessive
female sterile germline-dependent
female sterile soma-dependent | recessive
Other Phenotypes
Allele
abnormal neuroanatomy | larval stage, with Scer\GAL4elav-C155
some die during embryonic stage, with fs(1)N14
some die during embryonic stage, with fs(1)NΔ6
some die during embryonic stage | maternal effect
some die during embryonic stage | maternal effect (with fs(1)N12)
some die during embryonic stage | maternal effect (with fs(1)N14)
terminal phenotype | embryonic stage, with fs(1)N14
terminal phenotype | embryonic stage, with fs(1)NΔ6
terminal phenotype | embryonic stage | maternal effect (with fs(1)N12)
terminal phenotype | embryonic stage | maternal effect (with fs(1)N14)
wild-type
Phenotype manifest in
Allele
abdominal segment 5
abdominal segment 6
abdominal segment 7
abdominal segment 8
abdominal segment 8 | maternal effect
abdominal segment 9
abdominal segment 10
anterior fascicle & synapse, with Scer\GAL4elav-C155
anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155
cephalopharyngeal skeleton | maternal effect
egg
embryonic abdominal segment 8
embryonic abdominal segment 9
embryonic abdominal segment 10
embryonic telson
head | anterior | maternal effect
labrum
larval intersegmental nerve, with Scer\GAL4elav-C155
ocular segment
oocyte
proctodeum | embryonic stage
telson
telson | maternal effect
ventral furrow
vitelline membrane
vitelline membrane | maternal effect
wing vein L2
wing vein L3
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\ELSPBP1
1 of 14
Yes
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (0)
Mus musculus (laboratory mouse) (0)
Xenopus tropicalis (Western clawed frog) (0)
Danio rerio (Zebrafish) (0)
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP003290
10 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:fs(1)N. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
fs(1)M3
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Ppt1
    suppressible
    fs(1)N
    (Buff et al., 2007)
    Ppt1
    enhanceable
    fs(1)N
    (Buff et al., 2007)
    tsl
    suppressible
    fs(1)N
    (Furriols et al., 1998)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-0.1
    Cytogenetic map
    1F3-1F3
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    1F3-1F3
    Limits computationally determined from genome sequence between P{EP}EP1542 and P{EP}futschEP1419
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    1-0.1

    (FlyBase, 2016, Comeron et al., 2012)

    1-0.0

    Left of (cM)
    Right of (cM)
    Notes

    Closely linked to sc.

    Stocks and Reagents
    Stocks (12)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (29)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments

          fs(1)N and fs(1)M3 are necessary for extracellular accumulation of tsl.

          (Jimenez et al., 2002)

          The maternal terminal system is necessary to activate tll expression in the terminal caps.

          (Pignoni et al., 1992)

          Mature follicles are immunologically stained for asymmetric distribution of ecdysteroid-related antigen. During late oogenesis localisation of the antigen changes dramatically suggesting the antigen plays a role in early embryogenesis and, perhaps, in pattern formation.

          (Grau and Gutzeit, 1990)

          Involved in functions related to that of tll.

          (Strecker et al., 1988)

          Mutations of fs(1)N produce eggs with vitelline membrane defects and some embryos with defective development.

          (Perrimon et al., 1986)
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: fs(1)N EG:8D8.1

          (Jimenez et al., 2002)
          Additional comments
          Nomenclature History
          Source for database identify of
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (16)
          Reported As
          Symbol Synonym
          371
          (Komitopoulou et al., 1983)
          8D8.1
          (Kraut et al., 2001, Kraut et al., 2001)
          CG11411
          (National Institute of Genetics Fly Stocks, 2023-, Çiçek et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Fakhouri et al., 2006, Kraut et al., 2001, Kraut et al., 2001, Benos, 2000.12.13)
          EG:8D8.1
          (Benos et al., 2001)
          Fs(1)N
          (Buff et al., 2007)
          Nas
          (Airoldi and Cooley, 2007)
          fs(1)nas
          fs(1)A371
          (Merriam et al., 1991)
          fs(1)K1540
          (Mevel-Ninio et al., 1996, Perrimon et al., 1986, Perrimon and Gans, 1983)
          fs(1)M6
          (Perrimon et al., 1986, Mohler and Carroll, 1984)
          fs(1)N
          (Pignataro et al., 2024, Cridland et al., 2020, Parker et al., 2020, Santana et al., 2020, Statzer and Ewald, 2020, Mineo et al., 2017, Vazquez-Pianzola et al., 2017, Carnes et al., 2015, Jambor et al., 2015, Sopko and Perrimon, 2013, Grillo et al., 2012, Ventura et al., 2010, Christensen et al., 2008.6.11, Christensen et al., 2008.12.28, Turner et al., 2008, Georlette et al., 2007, Goodliffe et al., 2007, Matusek et al., 2006)
          fs(1)Nas
          (Ventura et al., 2010, Cernilogar et al., 2001, Furriols et al., 1998, Sprenger and Nusslein-Volhard, 1993, Merriam et al., 1991, Degelmann et al., 1990)
          fs(1)Nasrat
          (Tadros et al., 2007, LeMosy, 2003, Jimenez et al., 2002, Perrimon and Desplan, 1994, Lu et al., 1993, Finkelstein and Perrimon, 1991, Lipshitz, 1991, Perrimon et al., 1991, Nishida, 1990, Ambrosio et al., 1989)
          nas
          (Grillo et al., 2012)
          Name Synonyms
          Nasrat
          (Airoldi et al., 2011, Ventura et al., 2010, Ventura et al., 2008, Airoldi and Cooley, 2007, Airoldi and Cooley, 2006, Fakhouri et al., 2006, Airoldi and Cooley, 2005, Baek and Lee, 1999)
          female sterile (1) Nasrat
          (Carnes et al., 2015)
          fs(1)Nasrat
          (Ventura et al., 2010)
          Secondary FlyBase IDs
          • FBgn0000750
          • FBgn0000914
          • FBgn0024367
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 39 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          References (106)