FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Prat
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General Information
Symbol
Dmel\Prat
Species
D. melanogaster
Name
Phosphoribosylamidotransferase
Annotation Symbol
CG2867
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004901
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
amidophosphoribosyltransferase (2.4.2.14)
Gene Summary
Phosphoribosylamidotransferase (Prat) encodes a type-2 glutamine amidotransferase that converts phosphoribosylpyrophosphate to phosphoribosylamine using glutamine. It is an essential enzyme in the pathway for de novo synthesis of the purine nucleotide inosine monophosphate (IMP). IMP is the precursor for purine nucleotides required for nucleic acids, energy transfer, cell signaling, and coenzymes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
84E5-84E5
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:7909598..7911779
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables amidophosphoribosyltransferase activity
inferred from mutant phenotype
(Clark, 1994)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables amidophosphoribosyltransferase activity
inferred from electronic annotation with InterPro:IPR005854
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000225535
(GO Reference Genome Project, 2011-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in determination of adult lifespan
inferred from mutant phenotype
(Malmanche and Clark, 2004)
involved_in embryonic cleavage
inferred from mutant phenotype
(Malmanche and Clark, 2004)
involved_in oogenesis
inferred from mutant phenotype
(Malmanche and Clark, 2004)
involved_in purine nucleotide biosynthetic process
inferred from mutant phenotype
(Clark, 1994)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in purine nucleobase biosynthetic process
inferred from electronic annotation with InterPro:IPR005854
(InterPro Project Members, 2004-)
involved_in purine nucleotide biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000225535
(GO Reference Genome Project, 2011-)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
amidophosphoribosyltransferase activity
5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho- alpha-D-ribose 1-diphosphate + L-glutamine + H2O (2.4.2.14)
RHEA 14905:
Summaries
Gene Snapshot
Phosphoribosylamidotransferase (Prat) encodes a type-2 glutamine amidotransferase that converts phosphoribosylpyrophosphate to phosphoribosylamine using glutamine. It is an essential enzyme in the pathway for de novo synthesis of the purine nucleotide inosine monophosphate (IMP). IMP is the precursor for purine nucleotides required for nucleic acids, energy transfer, cell signaling, and coenzymes. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
AMIDOPHOSPHORIBOSYLTRANSFERASES -
Amidophosphoribosyltransferases catalyze the transfer of amido group from 5-phospho-beta-D-ribosylamine on to glutamate to form 5-phosphoribosyl-1-pyrophosphate (PRPP) and glutamine.
Protein Function (UniProtKB)
Involved in the first step (and regulatory point) of the de novo biosynthesis of purine nucleotides, where it catalyzes the transfer of glutamine amide to 5-phospho-alpha-D-ribose 1-diphosphate.
(UniProt, Q27601)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Prat for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q27601)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Prat-RA
FBtr0081764
NM_079547
2102
546
Prat-RB
FBtr0304705
NM_001260043
2105
546
Additional Transcript Data and Comments
Reported size (kB)

2.1 (northern blot)

(Clark, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Prat-PA
FBpp0081261
59.5
546
6.65
Q27601
NP_524271
AAF54163
Prat-PB
FBpp0293248
59.5
546
6.65
Q27601
NP_001246972
AFH06291
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

546 aa isoforms: Prat-PA, Prat-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

511 (aa)

(Clark, 1994)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prat using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.43

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
anal pad primordium

Comment: reported as anal pad specific anlage

(Fisher et al., 2012)
anterior midgut primordium
(Fisher et al., 2012)
fat body/gonad primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)
somatic muscle primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic anal pad
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic large intestine
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic/larval fat body
(Fisher et al., 2012)
embryonic/larval lymph gland
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
oogenesis
nurse cell
(Malmanche and Clark, 2004)
oogenesis stage S10
oocyte
(Malmanche and Clark, 2004)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- day 5 of adulthood
organism
(Malmanche et al., 2003)
adult stage | female
ovary
(Malmanche et al., 2003)
Additional Descriptive Data

Prat transcript is expressed at all developmental stages. Prat transcript is present at the earliest embryonic stages, indicating a maternally deposited component. Zygotic embryonic expression peaks between 4 and 12 hours AEL, and continues to be strongly expressed through the first larval instar. Prat transcript falls to low levels during the second larval instar, then increases through prepupal stages. At adult stages, Prat transcipt is expressed at much higher levels in females than males; the majority of the adult female expression is in the ovaries. The Prat developmental expression pattern is distinct from that of duplicate gene Prat2; the latter has no ovarian expression.

(Malmanche et al., 2003)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S1 -- S6
germline cell
(Malmanche and Clark, 2004)
nurse cell
(Malmanche and Clark, 2004)
follicle cell
(Malmanche and Clark, 2004)
oogenesis stage S10
main body follicle cell
(Malmanche and Clark, 2004)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prat in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Prat
Transgenic constructs containing regulatory region of Prat
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 11 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Act5C.PI
lethal | pharate adult stage P10 (with Df(3R)dsx43)
lethal | pharate adult stage P15, with Scer\GAL4αTub84B.PL
lethal | pharate adult stage P15 (with Df(3R)dsx43)
lethal | pupal stage, with Scer\GAL4αTub84B.PL
lethal | pupal stage P5, with Scer\GAL4αTub84B.PL
lethal | pupal stage P5 (with Df(3R)dsx43)
lethal | recessive
partially lethal (with Df(3R)dsx43)
partially lethal (with Prat12A19)
partially lethal (with Prat16A6)
partially lethal | maternal effect | conditional (with Df(3R)dsx43)
partially lethal | maternal effect | conditional (with Prat12A19)
partially lethal | maternal effect | conditional (with Prat16A6)
partially lethal - majority die (with Df(3R)dsx43)
partially lethal - majority die | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Act5C.PI
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile, with Scer\GAL4Act5C.PI
Other Phenotypes
Allele
increased cell death | prepupal stage P1 (with Df(3R)dsx43)
melanotic necrosis | pupal stage P5 (with Df(3R)dsx43)
short lived | dominant | female
short lived | female (with Df(3R)dsx43)
short lived | female (with Prat12A19)
short lived | female (with Prat16A6)
some die during pupal stage (with Df(3R)dsx43)
visible | recessive
Phenotype manifest in
Allele
abdominal tergite | pharate adult stage, with Scer\GAL4Act5C.PI
border follicle cell (with Df(3R)dsx43)
border follicle cell (with Prat12A19)
border follicle cell (with Prat16A6)
cleavage nucleus | maternal effect (with Df(3R)dsx43)
cleavage nucleus | maternal effect (with Prat12A19)
cleavage nucleus | maternal effect (with Prat16A6)
egg chamber (with Df(3R)dsx43)
egg chamber (with Prat12A19)
egg chamber (with Prat16A6)
embryo | embryonic cycle 6 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 6 | maternal effect (with Prat12A19)
embryo | embryonic cycle 6 | maternal effect (with Prat16A6)
embryo | embryonic cycle 7 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 7 | maternal effect (with Prat12A19)
embryo | embryonic cycle 7 | maternal effect (with Prat16A6)
embryo | embryonic cycle 8 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 8 | maternal effect (with Prat12A19)
embryo | embryonic cycle 8 | maternal effect (with Prat16A6)
embryo | embryonic cycle 9 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 9 | maternal effect (with Prat12A19)
embryo | embryonic cycle 9 | maternal effect (with Prat16A6)
embryo | embryonic cycle 10 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 10 | maternal effect (with Prat12A19)
embryo | embryonic cycle 10 | maternal effect (with Prat16A6)
embryo | embryonic cycle 11 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 11 | maternal effect (with Prat12A19)
embryo | embryonic cycle 11 | maternal effect (with Prat16A6)
embryo | embryonic cycle 12 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 12 | maternal effect (with Prat12A19)
embryo | embryonic cycle 12 | maternal effect (with Prat16A6)
embryo | embryonic cycle 13 | maternal effect (with Df(3R)dsx43)
embryo | embryonic cycle 13 | maternal effect (with Prat12A19)
embryo | embryonic cycle 13 | maternal effect (with Prat16A6)
embryonic abdominal segment | maternal effect (with Df(3R)dsx43)
follicle cell (with Df(3R)dsx43)
follicle cell (with Prat12A19)
follicle cell (with Prat16A6)
larval abdominal segment | maternal effect (with Df(3R)dsx43)
leg | pharate adult stage P10 (with Df(3R)dsx43)
nurse cell & nucleus (with Df(3R)dsx43)
nurse cell & nucleus (with Prat12A19)
nurse cell & nucleus (with Prat16A6)
oocyte (with Df(3R)dsx43)
oocyte (with Prat12A19)
oocyte (with Prat16A6)
polar body nucleus | maternal effect (with Df(3R)dsx43)
polar body nucleus | maternal effect (with Prat12A19)
polar body nucleus | maternal effect (with Prat16A6)
posterior crossvein
wing | pharate adult stage P10 (with Df(3R)dsx43)
wing disc | prepupal stage P1 (with Df(3R)dsx43)
yolk nucleus | maternal effect (with Df(3R)dsx43)
yolk nucleus | maternal effect (with Prat12A19)
yolk nucleus | maternal effect (with Prat16A6)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
Hsap\PPAT
14 of 14
Yes
Yes
1  
Hsap\GFPT1
1 of 14
No
No
Hsap\GFPT2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Ppat
14 of 14
Yes
Yes
Rnor\Gfpt1
1 of 14
No
No
Rnor\Gfpt2
1 of 14
No
No
Mus musculus (laboratory mouse) (3)
Mmus\Ppat
13 of 14
Yes
Yes
Mmus\Gfpt1
1 of 14
No
No
Mmus\Gfpt2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\ppat
8 of 13
Yes
Yes
Xtro\asns
1 of 13
No
No
Xtro\baalc
1 of 13
No
No
Xtro\gfpt1
1 of 13
No
No
Xtro\gfpt2
1 of 13
No
No
Xtro\krt55
1 of 13
No
Yes
Danio rerio (Zebrafish) (3)
Drer\ppat
14 of 14
Yes
Yes
Drer\gfpt1
1 of 14
No
No
Drer\gfpt2
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\ppat-1
13 of 14
Yes
Yes
Cele\gfat-1
1 of 14
No
No
Cele\gfat-2
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP000179
11 of 12
Yes
Yes
Agam\AgaP_AGAP011352
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\ASE1
12 of 13
Yes
Yes
Atha\ASE2
12 of 13
Yes
Yes
Atha\ASE3
12 of 13
Yes
Yes
Atha\ASN1
1 of 13
No
No
Atha\GFAT
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\ADE4
10 of 13
Yes
Yes
Scer\GFA1
1 of 13
No
No
Scer\YMR084W
1 of 13
No
No
Scer\YMR085W
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\ade4
10 of 12
Yes
Yes
Spom\SPBC12C2.11
1 of 12
No
No
Escherichia coli (enterobacterium) (2)
Ecol\purF
10 of 11
Yes
Yes
Ecol\glmS
1 of 11
No
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Prat. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (4)
Prat2
12 of 13
AsnS
1 of 13
Gfat1
1 of 13
Gfat2
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    PratHMS02669
    ameliorates  tauopathy
    modeled by Hsap\MAPTUAS.cUa
    (Yadav et al., 2024)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PPAT; phosphoribosyl pyrophosphate amidotransferase
    14 of 14
    172450
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Prat
        suppressible
        Prat2
        (Penney et al., 2008)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Ras85D|scrib
        suppressible
        Prat
        (Willoughby et al., 2013)
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-48
        Cytogenetic map
        84E5-84E5
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        84E5-84E5
        Limits computationally determined from genome sequence between P{EP}EP3060EP3060 and P{PZ}grn05930
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        84E1-84E2
        (determined by in situ hybridisation)
        (Clark, 1994, Clark and Henikoff, 1992)
        Experimentally Determined Recombination Data
        Location

        3-48

        (FlyBase, 2016)

        3-44.5

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (11)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (20)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Mutant adult females have a significantly shorter life span than normal, show reduced fertility that is conditional on diet and associated with alterations of egg chambers, and show a semi-lethal maternal effect that appears to be due to defects in nuclear proliferation during the early stages of embryonic development and results in segmental defects.

            (Malmanche and Clark, 2004)

            By comparing methylation by Ecol\dam methylase between euchromatic and heterochromatic genes it was determined that the heterochromatic state does not prevent methylase accessibility in vivo.

            (Wines et al., 1996)

            Prat function is an important regulatory point of the de novo purine pathway.

            (Clark, 1994)

            Prat encodes the enzyme that catalyses the first committed step and regulatory point in the de novo purine biosynthesis pathway.

            (Clark and Henikoff, 1992)
            Relationship to Other Genes
            Source for database merge of
            Additional comments

            The Prat gene may have been derived from the Prat2 gene by retroposition.

            (Langille and Clark, 2007, Langille and Clark, 2007, Malmanche et al., 2003)
            Nomenclature History
            Source for database identify of

            Source for identity of: Prat CG2867

            (Gong, 1999.11)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (6)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 52 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
            References (72)