Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of at least Clp, Cpsf73, Cpsf100 and Cpsf160.
The N-terminal region containing the five C3H1-type zinc fingers is essential for endonuclease activity.
The C-terminal region containing the two CCHC-type zinc fingers confers a binding preference for RNAs that contain G- and/or C-rich clusters.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Clp using the Feature Mapper tool.
In Northern blots, Clp expression is detected in early embryos and in ovaries. In situ hybridization shows that embryonic expression, which is detected until the cellular blastoderm stage, is ubiquitous. In the ovary, expression is detected starting at stage S3, in both oocytes and nurse cells. Oocyte expression stops being detected at stage S8, but resumes at stage S12, with the transfer of nurse cell contents to the oocyte. RNase protection assays show that the Clp transcript is expressed in all developmental stages, and is most abundant in adults. Maximal adult female expression is in the ovary. Amount of embryonic Clp transcript decreases during embryogenesis. A second peak of expression in third instar larvae decays to barely detectable levels in late pupae.
The Clp protein is detected in nurse cell nuclei up to stage S12 of oogenesis, and in the oocyte nucleus throughout oogenesis. Follicle cell nuclei also stain. Clp protein is also detected in the nuclei of all larval tissues. Salivary gland expression is initially restricted to the anterior of the gland and subsequently spreads to the rest of the gland. No Clp protein was detected in the embryo.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Clp in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Clp CG3642
The C-terminus of the Clp protein, containing two CCHC zinc knuckles, confers a binding preference for RNAs containing G- and/or C-rich clusters.