FlyBase Gene Report: Dmel\Or43b

General Information

Symbol

Dmel\Or43b

Species

D. melanogaster

Name

Odorant receptor 43b

Annotation Symbol

CG17853

Feature Type

protein_coding_gene

FlyBase ID

FBgn0026393

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Odorant receptor 43b (Or43b) encodes a multi-transmembrane chemoreceptor that mediates response to volatile chemicals. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

AN7, 43b

Key Links

Genomic Location

Cytogenetic map

43F2-43F2

Sequence location

2R:7,920,835..7,922,604 [-]

Recombination map

2-57

RefSeq locus

NT_033778 REGION:7920835..7922604

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables ionotropic olfactory receptor activity

inferred from high throughput direct assay

(Hallem and Carlson, 2006)

enables odorant binding

inferred from mutant phenotype

(Elmore et al., 2003)

enables olfactory receptor activity

inferred from mutant phenotype

(Root et al., 2007)

inferred from direct assay

(Marshall et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables odorant binding

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

enables olfactory receptor activity

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Clyne et al., 1999, Gao and Chess, 1999)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in detection of chemical stimulus involved in sensory perception of smell

inferred from direct assay

(Marshall et al., 2010)

involved_in sensory perception of smell

inferred from mutant phenotype

(Keller and Vosshall, 2007, Root et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in detection of chemical stimulus involved in sensory perception of smell

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

involved_in sensory perception of smell

non-traceable author statement

(Clyne et al., 1999, Gao and Chess, 1999)

traceable author statement

(Carlson, 2001)

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from high throughput direct assay

(Hallem and Carlson, 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in dendrite membrane

inferred from sequence or structural similarity with FLYBASE:Or22a; FB:FBgn0026398

(Gene Ontology Curators, 2002-)

located_in membrane

non-traceable author statement

(Clyne et al., 1999, Gao and Chess, 1999)

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ODORANT RECEPTORS

Protein Family (UniProt)

Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a subfamily. (P81918)

Protein Signatures (InterPro)

Olfactory receptor, insect

Summaries

Gene Snapshot

Odorant receptor 43b (Or43b) encodes a multi-transmembrane chemoreceptor that mediates response to volatile chemicals. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

ODORANT RECEPTORS -: Insect odorant receptors (ORs) are a family of chemoreceptors activated by volatile chemicals. ORs form heteromeric complexes, believed to function as non-selective cation channels, comprising an OR and the co-receptor Orco. ORs are expressed in the dendrites of subsets of olfactory neurons in the antenna and maxillary palp. (Adapted from FBrf0168007 and FBrf0220463).

Protein Function (UniProtKB)

Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. May form a complex with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability.

(UniProt, P81918)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Or43b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P81918)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.41

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Or43b-RA

FBtr0088887

NM_078932

1412

403

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Or43b-PA

FBpp0087962

46.6

403

8.56

P81918

NP_523656

AAF59173

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

426 (aa)

(Gao and Chess, 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway (By similarity).

(UniProt, P81918)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Olfactory receptor, insect

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P81918

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Or43b using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Or43b_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.61

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

sensory neuron of third segment of antenna

(Gao and Chess, 1999)

adult olfactory receptor neuron Or43b

(Couto et al., 2005)

Additional Descriptive Data

Or43b transcript is detected in a small subset of neurons in antennal segment 3.

(Gao and Chess, 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from high throughput direct assay

(Hallem and Carlson, 2006)

Expression Deduced from Reporters

Reporter: P{Or43b-GAL4.C}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antennal basiconic sensillum ab8

(Larter et al., 2016, Couto et al., 2005)

adult olfactory receptor neuron Or43b

(Couto et al., 2005)

Reporter: P{Or43b-Mmus\Cd8a.GFP}

Stage

Tissue/Position (including subcellular localization)

Reference

P-stage

antennal lobe glomerulus DM5

(Sakurai et al., 2009)

antennal lobe glomerulus VM2

(Sakurai et al., 2009)

adult stage

adult olfactory receptor neuron Or43b

(Couto et al., 2005)

antennal basiconic sensillum ab8

(Couto et al., 2005)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Or43b in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Or43b

View exonic expression by tissue for Dmel\Or43b

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35743/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026393

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026393

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026393

Flygut - An atlas of the Drosophila adult midgut

FBgn0026393

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Or43b

Transgenic constructs containing regulatory region of Or43b

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6055

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Or43b^GD1857

viable, with Scer\GAL4^Mef2.PR

Or43b^GD1857

viable, with Scer\GAL4^pnr-MD237

Or43b^GD1857

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal smell perception

Phenotype manifest in

Allele

basiconic sensillum

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (77)

Agam\GPROR15

5 of 12

Yes

Agam\GPROR30

5 of 12

Yes

Agam\GPROR53

5 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

Yes

Agam\GPROR25

3 of 12

Yes

Agam\GPROR27

3 of 12

Agam\GPROR28

3 of 12

Yes

Agam\GPROR29

3 of 12

Agam\AgaP_AGAP005495

2 of 12

Agam\AgaP_AGAP013396

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Agam\GPROR63

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\GPROR60

1 of 12

Agam\GPROR61

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Or43b. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (59)

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

6 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway (By similarity).

(UniProt, P81918 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0026393

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-57

Cytogenetic map

43F2-43F2

Sequence location

2R:7,920,835..7,922,604 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43F2-43F2

Limits computationally determined from genome sequence between P{lacW}lin19^k01207&P{lacW}rnh1^k07624 and P{lacW}CSN4^k08018&P{lacW}l(2)k08504^k08504

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43F-43F

(determined by in situ hybridisation)

(Elmore and Smith, 2001)

Experimentally Determined Recombination Data

Location

2-57

(FlyBase, 2016)

2-57.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

76043

w^*; P{UAS-Or43b.MYC}1

23894

w^*; P{Or43b-GAL4.C}110t8.1

23895

w^*; P{Or43b-GAL4.C}110t6.3

97369

w^*; TI{TI}Or43b^1.w-

94453

w^*; TI{CRIMIC.TG4.2}Or43b^{CR60441-TG4.2}

57769

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04963}attP40

NIG-Fly

2RG-0821

y² VhaAC39-1^cho-1 v¹; P{U6-Or43b.NIG.sgRNA}attP40/CyO

VDRC

v109777

P{KK105017}VIE-260B

v7360

w¹¹¹⁸; P{GD1857}v7360

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP14440

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026393

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026393

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Or25A.1 CG17853

(Vosshall, 1999.11)

Source for identity of: Or43b Or25A.1

(Warr and Vosshall, 2000.5.19)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

25A.1/43

(Warr and Vosshall, 2000.5.19)

43b

(Alkhori et al., 2014, Vogt et al., 2002)

AN7

(Warr and Vosshall, 2000.5.19, Gao and Chess, 1999)

CG17853

(National Institute of Genetics Fly Stocks, 2022-, Ni et al., 2010.12.1, Zhang et al., 2010, Keleman et al., 2009.8.5, Robertson, 2009, Warr and Vosshall, 2000.5.19)

DOR25A.1

(Clyne et al., 1999)

DOR81

(Warr and Vosshall, 2000.5.19)

OR43b

(Walker et al., 2023, Das et al., 2019, Chow et al., 2011, Störtkuhl and Fiala, 2011, Elmore and Smith, 2001)

Or25A.1

Or43b

(Smith, 2023.2.13, Ali et al., 2022, Mier et al., 2022, McLaughlin et al., 2021, Chepurwar et al., 2019, He et al., 2019, Gene Disruption Project members, 2018-, Crocker et al., 2016, Grabe et al., 2016, Menuz et al., 2014, Bachtiar et al., 2013, Gabler et al., 2013, Martin et al., 2013, Jafari et al., 2012, Kazama et al., 2011, Lieber et al., 2011, de Bruyne et al., 2010, Galizia et al., 2010, Guo and Kim, 2010, Kong et al., 2010, Marshall et al., 2010, Stökl et al., 2010, Robertson, 2009, Zhou et al., 2009, Kopp et al., 2008, Olsen and Wilson, 2008, Smart et al., 2008, Guo and Kim, 2007, Keller and Vosshall, 2007, Lattemann et al., 2007, McBride et al., 2007, Olsen et al., 2007, Reiter et al., 2007, Root et al., 2007, de Bruyne and Warr, 2006, Hallem and Carlson, 2006, Kreher et al., 2005, Robertson et al., 2003)

Name Synonyms

Odorant receptor 43b

Olfactory receptor 25A.1

(Clyne et al., 1999)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 23 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35743

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF59173

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P81918

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P81918

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P81918

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35743/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026393

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026393

FlyMine - An integrated database for Drosophila genomics

FBgn0026393

InterPro - A database of protein families, domains and functional sites

Olfactory receptor, insect

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17853

MARRVEL_MODEL - MARRVEL (model organism gene)

35743

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026393

DroID - A comprehensive database of gene and protein interactions.

FBgn0026393

DRSC - Results frm RNAi screens

FBgn0026393

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Or43b_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026393

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0026393

Flygut - An atlas of the Drosophila adult midgut

FBgn0026393

References (95)

The following external sites may use different assemblies or annotations than FlyBase.

-0.61

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