FlyBase Gene Report: Dmel\aust

General Information

Symbol

Dmel\aust

Species

D. melanogaster

Name

australin

Annotation Symbol

CG17009

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032104

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

australin (aust) is a male meiotic specific paralogue of borr, which encodes one of the three targeting subunits for the product of aurB in the chromosomal passenger complex (CPC). The CPC is critical in regulating multiple aspects of cell division, including chromosome condensation, kinetochore function and cytokinesis, through the kinase activity of the product of aurB. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

30A6-30A6

Sequence location

2L:9,250,048..9,251,042 [-]

Recombination map

2-35

RefSeq locus

NT_033779 REGION:9250048..9251042

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables DNA binding

inferred from direct assay

(Gao et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in male meiosis chromosome segregation

inferred from mutant phenotype

(Gao et al., 2008)

involved_in male meiosis cytokinesis

inferred from mutant phenotype

(Gao et al., 2008)

involved_in male meiosis sister chromatid cohesion

inferred from mutant phenotype

(Gao et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mitotic sister chromatid segregation

inferred from biological aspect of ancestor with PANTHER:PTN001815752

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of chromosome passenger complex

inferred from direct assay

(Gao et al., 2008)

located_in kinetochore

inferred from direct assay

(Gao et al., 2008)

located_in meiotic spindle midzone

inferred from direct assay

(Gao et al., 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of chromosome passenger complex

inferred from biological aspect of ancestor with PANTHER:PTN001815752

(GO Reference Genome Project, 2011-)

is_active_in chromosome, centromeric region

inferred from biological aspect of ancestor with PANTHER:PTN001815752

(GO Reference Genome Project, 2011-)

is_active_in spindle midzone

inferred from biological aspect of ancestor with PANTHER:PTN001815752

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

MEIOTIC CHROMOSOMAL PASSENGER COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MEIOTIC CHROMOSOMAL PASSENGER COMPLEX -: The Meiotic Chromosomal Passenger Complex plays several key roles in meiosis including kinetochore-microtubule attachment, spindle assembly and cytokinesis. (Adapted from FBrf0216422 and FBrf0202888).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\aust for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VLD7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

aust-RA

FBtr0079819

NM_135434

889

216

aust-RB

FBtr0343928

NM_001298846

929

216

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

aust-PA

FBpp0079418

24.6

216

11.44

FBpp0310419

24.6

216

11.44

Q9VLD7

NP_001285775

AHN54289

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

216 aa isoforms: aust-PA, aust-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VLD7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\aust using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

aust_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.67

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage | male

testis

(Gao et al., 2008)

adult stage | male

testis

(Gao et al., 2008)

Additional Descriptive Data

aust transcript is expressed only in the testis.

(Gao et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

primary spermatocyte

(Gao et al., 2008)

Additional Descriptive Data

aust protein is associated with the chromosomes of meiotic male germline cells.

(Gao et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of chromosome passenger complex

inferred from direct assay

(Gao et al., 2008)

located_in kinetochore

inferred from direct assay

(Gao et al., 2008)

located_in meiotic spindle midzone

inferred from direct assay

(Gao et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\aust in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\aust

View exonic expression by tissue for Dmel\aust

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34244/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032104

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032104

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032104

Flygut - An atlas of the Drosophila adult midgut

FBgn0032104

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of aust

Transgenic constructs containing regulatory region of aust

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2L)30A-C

(Gao et al., 2008)

Df(2L)N22-3

(Gao et al., 2008)

Df(2L)N22-5

(Gao et al., 2008)

Gene not disrupted in

Df(2L)γ7

(Gao et al., 2008)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

aust^GD9257

viable, with Scer\GAL4^Mef2.PR

aust^GD9257

viable, with Scer\GAL4^pnr-MD237

aust^GD9257

Sterility

Allele

male sterile (with Df(2L)30A-C)

aust¹

Other Phenotypes

Allele

abnormal cytokinesis | male

aust¹

abnormal cytokinesis | male (with Df(2L)30A-C)

aust¹

abnormal meiotic cell cycle | male

aust¹

abnormal meiotic cell cycle | male (with Df(2L)30A-C)

aust¹

visible, with Scer\GAL4^pnr-MD237

aust^GD9257

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

aust^GD9257

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\CDCA8

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Cdca8

1 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Cdca8

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\cdca8

1 of 13

Yes

Xtro\cdca9

1 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\cdca8

1 of 14

Yes

Drer\cdca9

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP011220

7 of 12

Yes

Agam\AgaP_AGAP011219

6 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:aust. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

borr

7 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

aust

pull down, western blot

(Gao et al., 2008)

aust - Incenp

pull down, western blot

(Gao et al., 2008)

aust - borr

pull down, western blot

(Gao et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60351

DroID - A comprehensive database of gene and protein interactions.

FBgn0032104

MIST (protein-protein) - An integrated Molecular Interaction Database

34244

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SUMOylation of DNA replication proteins

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-35

Cytogenetic map

30A6-30A6

Sequence location

2L:9,250,048..9,251,042 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30A6-30A6

Limits computationally determined from genome sequence between P{PZ}tai⁰¹³⁵¹ and P{PZ}scat¹&P{PZ}numb⁰³²³⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-35

(FlyBase, 2016)

2-30.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

NIG-Fly

17009R-2

P{NIG.17009R}2

M2L-0567

y¹ w¹¹¹⁸; aust^SK1 P{neoFRT}40A/CyO-Tb

M2L-0568

y¹ w¹¹¹⁸; aust^SK6 P{neoFRT}40A/CyO-Tb

2LG-0452

y² VhaAC39-1^cho-1 v¹; P{U6-aust.NIG.sgRNA}attP40/CyO

VDRC

v102332

P{KK111359}VIE-260B

v32809

w¹¹¹⁸; P{GD9257}v32809

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032104

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

IP08234

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032104

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The borr protein is a chromosomal passenger protein in all mitotic tissues studied, but is specifically replaced by aust protein for the two male meiotic divisions.

(Gao et al., 2008)

aust is required specifically during male meiosis

(Gao et al., 2008)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: aust CG17009

(Wakefield, 2006.5.24)

Nomenclature comments

Etymology

The gene is named "australin" after the aurora australis, or southern lights, due to its homology to the "borealin" gene (which is named after the aurora borealis, or northern lights).

(Gao et al., 2008)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Aust

(Giansanti and Fuller, 2012)

CG17009

(National Institute of Genetics Fly Stocks, 2022-, Keleman et al., 2009.8.5, Gao et al., 2008, Giot et al., 2003)

aust

(Frappaolo et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Giansanti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lesch and Page, 2012, Gao et al., 2008)

Name Synonyms

Australin

(Giansanti and Fuller, 2012)

australin

(Giansanti et al., 2012, Lesch and Page, 2012, Gao et al., 2008, Wakefield, 2006.5.24)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34244

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VLD7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VLD7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VLD7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34244/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032104

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032104

FlyMine - An integrated database for Drosophila genomics

FBgn0032104

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17009

MARRVEL_MODEL - MARRVEL (model organism gene)

34244

Linkouts

BioGRID - A database of protein and genetic interactions.

60351

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032104

DroID - A comprehensive database of gene and protein interactions.

FBgn0032104

DRSC - Results frm RNAi screens

FBgn0032104

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

aust_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032104

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032104

Flygut - An atlas of the Drosophila adult midgut

FBgn0032104

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032104

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030702

MIST (protein-protein) - An integrated Molecular Interaction Database

34244

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SUMOylation of DNA replication proteins

References (21)

The following external sites may use different assemblies or annotations than FlyBase.

4.67

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