FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Cen
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General Information
Symbol
Dmel\Cen
Species
D. melanogaster
Name
Centrocortin
Annotation Symbol
CG1962
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032876
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Summary
Centrocortin (Cen) encodes a protein that regulates cleavage furrow assembly in the early embryo. It localizes to centrosomes asymmetrically, and to cortical actin structures. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

38E.1

Key Links
Genomic Location
Cytogenetic map
38D5-38D5
Sequence location
Recombination map
2-54
RefSeq locus
NT_033779 REGION:20678921..20681846
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:cnn; FB:FBgn0013765
(Kao and Megraw, 2009)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in embryonic cleavage
inferred from mutant phenotype
(Kao and Megraw, 2009)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Bergalet et al., 2020, Kao and Megraw, 2009)
located_in cleavage furrow
inferred from direct assay
(Kao and Megraw, 2009)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Centrocortin (Cen) encodes a protein that regulates cleavage furrow assembly in the early embryo. It localizes to centrosomes asymmetrically, and to cortical actin structures. [Date last reviewed: 2019-03-07]
Summary (Interactive Fly)

cooperates with centrosomin to organize embryonic cleavage furrows - mRNA is localized to centrosomes - RNA localization to centrosomes is regulated during the cell cycle and developmentally - fragile-X mental retardation protein regulates centrocortin mRNA - convergently transcribed with ik2 as cis-natural antisense transcripts with overlapping 3' UTRs - a normalized local concentration of cen mRNA is essential for normal cell division and genome stability

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Cen for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VIK6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Cen-RA
FBtr0081399
NM_136205
2841
790
Cen-RB
FBtr0081401
NM_165338
2706
790
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Cen-PA
FBpp0080929
89.9
790
5.40
Q9VIK6
NP_610049
AAF53912
Cen-PB
FBpp0080931
89.9
790
5.40
Q9VIK6
NP_724279
AAN11083
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

790 aa isoforms: Cen-PA, Cen-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cen using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.81

Transcript Expression
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 9
organism
(Kao and Megraw, 2009)
adult stage | female
ovary
(Kao and Megraw, 2009)
Additional Descriptive Data

CEN is detected on western blots at high levels in ovaries and early embryos and then drops to very low levels in late embryos and in larval stages.

(Kao and Megraw, 2009)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Bergalet et al., 2020, Kao and Megraw, 2009)
located_in cleavage furrow
inferred from direct assay
(Kao and Megraw, 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cen in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Cen
Transgenic constructs containing regulatory region of Cen
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2L)Fs(2)Ket-RX32
(Kao and Megraw, 2009)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\CDR2L
8 of 14
Yes
Yes
Hsap\CDR2
7 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Cdr2l
10 of 14
Yes
Yes
Rnor\Cdr2
10 of 14
Yes
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Cdr2l
10 of 14
Yes
Yes
Mmus\Cdr2
8 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\cdr2
7 of 13
Yes
Yes
Xtro\cdr2l
6 of 13
No
Yes
Xtro\lrrtm1
1 of 13
No
No
Xtro\thap11
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\cdr2l
10 of 14
Yes
Yes
Drer\cdr2a
9 of 14
No
Yes
Drer\cdr2b
9 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP000264
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Cen. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CDR2L; cerebellar degeneration related protein 2 like
    8 of 14
        CDR2; cerebellar degeneration related protein 2
        7 of 14
        117340
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            RNA-RNA
            Physical Interaction
            Assay
            References
            Cen - IKKε
            pull down, quantitative reverse transcription pcr
            (Bergalet et al., 2020)
            RNA-protein
            Physical Interaction
            Assay
            References
            Cen
            anti bait coimmunoprecipitation, quantitative reverse transcription pcr
            (Ryder et al., 2020)
            Cen - Fmr1
            anti bait coimmunoprecipitation, western blot, quantitative reverse transcription pcr, anti tag coimmunoprecipitation
            (Ryder et al., 2020)
            protein-protein
            Physical Interaction
            Assay
            References
            Cen - Fgop2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - Fmr1
            anti bait coimmunoprecipitation, western blot, quantitative reverse transcription pcr, anti tag coimmunoprecipitation
            (Ryder et al., 2020)
            Cen - RluA-2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - Slmap
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - Tlk
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - Ubqn
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - ana2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - cmet
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - cnn
            two hybrid
            (Kao and Megraw, 2009)
            Cen - dup
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - geminin
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - nxf2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - row
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cen - woc
            experimental knowledge based
            (Guruharsha et al., 2011)
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Cen
            enhanceable
            cnn
            (Kao and Megraw, 2009)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Fmr1
            suppressible
            Cen
            (Ryder et al., 2020)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2L
            Recombination map
            2-54
            Cytogenetic map
            38D5-38D5
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            38D5-38D5
            Limits computationally determined from genome sequence between P{lacW}k02501&P{lacW}k14810 and P{PZ}Hr3802306&P{PZ}dia1
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            2-54

            (FlyBase, 2016)

            2-54.1

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (9)
            Genomic Clones (19)
            cDNA Clones (89)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Kao and Megraw, 2009)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of

                Source for identity of: Cen CG1962

                (Kao and Megraw, 2009)
                Nomenclature comments
                Etymology

                Named 'Centrocortin' because the protein associates with the centrosome and the cortex.

                (Kao and Megraw, 2009)
                Synonyms and Secondary IDs (6)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 39 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                MIST (protein-protein) - An integrated Molecular Interaction Database
                References (41)