FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\NUCB1
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General Information
Symbol
Dmel\NUCB1
Species
D. melanogaster
Name
Nucleobindin 1
Annotation Symbol
CG32190
Feature Type
protein_coding_gene
FlyBase ID
FBgn0052190
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
NUCB1 (NUCB1) encodes a protein involved in the defense response to Gram-negative bacteria. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
75A2-75A2
Sequence location
Recombination map
3-45
RefSeq locus
NT_037436 REGION:17797738..17802279
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables calcium ion binding
inferred from biological aspect of ancestor with PANTHER:PTN001059206
(GO Reference Genome Project, 2011-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in defense response to Gram-negative bacterium
inferred from mutant phenotype
(Berkey et al., 2009)
involved_in insemination
inferred from high throughput expression pattern
(Findlay et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in carbohydrate metabolic process
inferred from electronic annotation with InterPro:IPR011330
(InterPro Project Members, 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2008)
located_in Golgi apparatus
inferred from direct assay
(Otte et al., 1999)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in endoplasmic reticulum-Golgi intermediate compartment
inferred from biological aspect of ancestor with PANTHER:PTN001059206
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
NUCB1 (NUCB1) encodes a protein involved in the defense response to Gram-negative bacteria. [Date last reviewed: 2019-09-26]
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\NUCB1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VVK7)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
NUCB1-RA
FBtr0075171
NM_140751
2203
569
Additional Transcript Data and Comments
Reported size (kB)

1.806 (longest cDNA)

(Otte et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
NUCB1-PA
FBpp0074937
67.4
569
4.98
Q9VVK7
NP_649008
AAF49304
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

534 (aa)

(Otte et al., 1999)

569 (aa); 67 (kD predicted)

(Otte et al., 1999)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NUCB1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.69

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous | uniform
(Otte et al., 1999)
organism

Comment: maternally deposited

(Fisher et al., 2012)
blastoderm stage
organism | ubiquitous
(Otte et al., 1999)
embryonic stage 4 -- 10
organism | ubiquitous
(Otte et al., 1999)
embryonic stage 11 -- 12
salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)
embryonic stage 11 -- 17
embryonic/larval salivary gland
(Otte et al., 1999)
salivary gland body primordium
(Otte et al., 1999)
embryonic stage 13 -- 16
embryonic/larval salivary gland
(Fisher et al., 2012)
embryonic/larval salivary gland body
(Fisher et al., 2012)
Additional Descriptive Data

NUCB1 transcripts are ubiquitously distributed in early embryos. From stage 11 onwards, high levels of transcript are observed in the placodes of the salivary glands. Salivary gland expression persists until the end of embryogenesis.

(Otte et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
embryonic/larval salivary gland
(Otte et al., 1999)
embryonic stage 1 -- 3
organism | ubiquitous | uniform
(Otte et al., 1999)
embryonic stage 4 -- 5
cell

Comment: possibly Golgi-localized

(Otte et al., 1999)
embryonic stage 4 -- 10
organism | ubiquitous
(Otte et al., 1999)
embryonic stage 11 -- 17
salivary gland body primordium
(Otte et al., 1999)
embryonic stage 17
embryonic/larval salivary gland
(Otte et al., 1999)
S2 cell-line
(Otte et al., 1999)
S2 cell-line
(Otte et al., 1999)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

NUCB1 protein is detected in blastoderm stage embryos. From stage 11 on it is expressed in the salivary glands.

(Otte et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2008)
located_in Golgi apparatus
inferred from direct assay
(Otte et al., 1999)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\NUCB1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of NUCB1
Transgenic constructs containing regulatory region of NUCB1
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel9005
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\NUCB2
12 of 14
Yes
Yes
Hsap\NUCB1
10 of 14
No
Yes
Hsap\CLMP
1 of 14
No
Yes
Hsap\NCR3LG1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Nucb2
12 of 14
Yes
Yes
Rnor\Nucb1
9 of 14
No
Yes
Mus musculus (laboratory mouse) (3)
Mmus\Nucb2
11 of 14
Yes
Yes
Mmus\Nucb1
9 of 14
No
Yes
Mmus\Clmp
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\nucb2
6 of 13
Yes
Yes
Xtro\nucb1
5 of 13
No
Yes
Xtro\LOC101731190
1 of 13
No
Yes
Danio rerio (Zebrafish) (3)
Drer\nucb2a
12 of 14
Yes
Yes
Drer\nucb2b
11 of 14
No
Yes
Drer\nucb1
10 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\nucb-1
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP005608
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SSP120
3 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPCC613.03
2 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:NUCB1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    NUCB2; nucleobindin 2
    12 of 14
    608020
        NUCB1; nucleobindin 1
        10 of 14
        601323
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Orai|NUCB1|Itpr
            suppressible
            SERCA
            (Balasubramanian and Srinivasan, 2020)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Itpr
            suppressible
            NUCB1
            (Balasubramanian and Srinivasan, 2020)
            Itpr
            suppressible
            Orai|NUCB1
            (Balasubramanian and Srinivasan, 2020)
            Itpr
            suppressible
            NUCB1|SERCA
            (Balasubramanian and Srinivasan, 2020)
            norpA
            suppressible
            NUCB1
            (Balasubramanian and Srinivasan, 2020)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3L
            Recombination map
            3-45
            Cytogenetic map
            75A2-75A2
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            75A2-75A2
            Limits computationally determined from genome sequence between P{lacW}l(3)j11B2j11B2 and P{PZ}W05014
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            75A3-75A7
            (Otte et al., 1999, Otte, 1997.11.17)
            Experimentally Determined Recombination Data
            Location

            3-45

            (FlyBase, 2016)

            3-41.2

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (8)
            Genomic Clones (7)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (23)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                Antibody Information
                Laboratory Generated Antibodies
                 

                polyclonal

                (Yeom et al., 2021, Otte et al., 1999)
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                    Other Comments
                    Relationship to Other Genes
                    Source for database merge of
                    Additional comments

                    Annotation CG5587 split into CG32186 and CG32190 (NUCB1) in release 3 of the genome annotation.

                    (FlyBase Genome Annotators, 2002-2003)
                    Nomenclature History
                    Source for database identify of
                    Nomenclature comments
                    Etymology
                    Synonyms and Secondary IDs (7)
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 48 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    FlyMine - An integrated database for Drosophila genomics
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Flygut - An atlas of the Drosophila adult midgut
                    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    References (54)