FlyBase Gene Report: Dmel\Ndae1

General Information

Symbol

Dmel\Ndae1

Species

D. melanogaster

Name

Na⁺-driven anion exchanger 1

Annotation Symbol

CG42253

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259111

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Na[+]-driven anion exchanger 1 (Ndae1) encodes an intrinsic membrane protein that reversibly mediates the exchange of 1 Na[+] and 2 HCO[[3]][-] for 1 Cl[-] at basolateral membranes of gut and renal epithelia as well as CNS neurons. In the CNS, the product of Ndae1 can lower resting intracellular Cl[-] controlling GABA(A) Cl-channel activity. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(2)k10316

Key Links

Genomic Location

Cytogenetic map

27E5-27E7

Sequence location

2L:7,223,328..7,249,343 [-]

Recombination map

2-25

RefSeq locus

NT_033779 REGION:7223328..7249343

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sodium:bicarbonate symporter activity

inferred from direct assay

(Romero et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables monoatomic anion transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR013769

(InterPro Project Members, 2004-)

enables sodium:bicarbonate symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000155503

(GO Reference Genome Project, 2011-)

enables solute:inorganic anion antiporter activity

inferred from electronic annotation with InterPro:IPR003020, InterPro:IPR003024

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001717, InterPro:IPR003020

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in bicarbonate transport

inferred from direct assay

(Romero et al., 2000)

involved_in chloride transmembrane transport

inferred from direct assay

(Romero et al., 2000)

involved_in proton transmembrane transport

inferred from direct assay

(Romero et al., 2000)

involved_in regulation of cellular pH

inferred from direct assay

(Romero et al., 2000)

involved_in sodium ion transmembrane transport

inferred from direct assay

(Romero et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in bicarbonate transport

inferred from biological aspect of ancestor with PANTHER:PTN000894302

(GO Reference Genome Project, 2011-)

involved_in monoatomic anion transport

inferred from electronic annotation with InterPro:IPR003020, InterPro:IPR003024, InterPro:IPR011531, InterPro:IPR013769

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001717, InterPro:IPR003020, InterPro:IPR011531, InterPro:IPR013769

(InterPro Project Members, 2004-)

involved_in regulation of intracellular pH

inferred from biological aspect of ancestor with PANTHER:PTN000155503

(GO Reference Genome Project, 2011-)

involved_in transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN004260927

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Sciortino et al., 2001)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR003020, InterPro:IPR003024, InterPro:IPR011531, InterPro:IPR013769

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001717, InterPro:IPR003020, InterPro:IPR011531, InterPro:IPR013769

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN004260927

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred by curator from GO:0008510

(Romero et al., 2000)

Gene Group (FlyBase)

SLC4 FAMILY OF BICARBONATE TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

sodium:bicarbonate symporter activity
RHEA 70267:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC4 FAMILY OF BICARBONATE TRANSPORTERS -: The solute carrier family 4 (SLC4) members are bicarbonate transmembrane transporters. SLC4 members are co-transporters that exchange Na⁺ or Cl^- for HCO₃^- across the plasma membrane. They are primarily involved in acid-base homeostasis. (Adapted from FBrf0130048 and PMID:23506864).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ndae1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IPI3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Low-frequency RNA-Seq exon junction(s) not annotated.

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ndae1-RA

FBtr0299537

NM_078777

3839

1030

Ndae1-RB

FBtr0299538

NM_164744

4046

1099

Ndae1-RC

FBtr0299539

NM_164743

4517

1190

Ndae1-RD

FBtr0331658

NM_001273248

4218

1134

Ndae1-RE

FBtr0331659

NM_001273249

4563

1086

Ndae1-RF

FBtr0331660

NM_001273250

3714

1027

Ndae1-RG

FBtr0331661

NM_001273251

3950

1067

Ndae1-RH

FBtr0331662

NM_001273252

4232

1053

Ndae1-RI

FBtr0331663

NM_001273253

4127

1126

Ndae1-RJ

FBtr0331664

NM_001273254

3735

1046

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ndae1-PA

FBpp0288812

115.0

1030

6.09

FBpp0288813

121.8

1099

6.75

FBpp0288814

131.6

1190

6.67

FBpp0304048

125.8

1134

6.98

FBpp0304049

120.2

1086

6.88

FBpp0304050

114.6

1027

6.34

FBpp0304051

118.4

1067

5.67

FBpp0304052

117.3

1053

6.03

FBpp0304053

124.5

1126

6.54

FBpp0304054

116.7

1046

5.91

M9PC95

NP_001260183

AGB92719

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ndae1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.83

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

foregut anlage in statu nascendi

(Fisher et al., 2012)

mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 6

cephalic furrow

(Romero et al., 2000)

mesoderm

(Romero et al., 2000)

posterior endoderm

(Romero et al., 2000)

hindgut anlage

(Romero et al., 2000)

ventral ectoderm

(Romero et al., 2000)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic anal pad

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 16 -- 17

larval central nervous system | restricted

(Romero et al., 2000)

larval peripheral nervous system | restricted

(Romero et al., 2000)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

Comment: assayed 0-19 hr AEL

(Romero et al., 2000)

P-stage

organism

(Romero et al., 2000)

adult stage | male

thorax

(Romero et al., 2000)

adult stage | female

thorax

(Romero et al., 2000)

adult stage

head

(Romero et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult aorta | subset

(Monier et al., 2005)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Ndae1 is expressed in tin-expressing myocytes in segments A1-A4 in adults.

(Monier et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Sciortino et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ndae1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ndae1

View exonic expression by tissue for Dmel\Ndae1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259111

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34005/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259111

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259111

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259111

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4675

Flygut - An atlas of the Drosophila adult midgut

FBgn0259111

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ndae1

Transgenic constructs containing regulatory region of Ndae1

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2L)Exel6017

(Ryder, 2004.4.26)

Gene not disrupted in

Df(2L)spd

(BDGP Project Members, 1994-1999)

Df(2L)XE-2750

(BDGP Project Members, 1994-1999)

Df(2L)XE-3801

(BDGP Project Members, 1994-1999)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Ndae1^k10316

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ndae1^GD967

viable, with Scer\GAL4^Mef2.PR

Ndae1^GD967

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Ndae1^GD967

Phenotype manifest in

Allele

imaginal disc

Ndae1^k10316

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (10)

Hsap\SLC4A7

13 of 14

Yes

Hsap\SLC4A8

13 of 14

Yes

Hsap\SLC4A10

13 of 14

Yes

Hsap\SLC4A4

10 of 14

Yes

7 of 14

7 of 14

7 of 14

7 of 14

Yes

Hsap\SLC4A9

7 of 14

Yes

Hsap\SLC4A11

3 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Slc4a7

13 of 14

Yes

Rnor\Slc4a8

13 of 14

Yes

Rnor\Slc4a10

13 of 14

Yes

Rnor\Slc4a4

10 of 14

Yes

Rnor\Slc4a5

8 of 14

Yes

7 of 14

7 of 14

7 of 14

7 of 14

Yes

Rnor\Slc4a11

3 of 14

Yes

Mus musculus (laboratory mouse) (10)

Mmus\Slc4a7

13 of 14

Yes

Mmus\Slc4a8

13 of 14

Yes

Mmus\Slc4a10

13 of 14

Yes

Mmus\Slc4a4

9 of 14

Yes

Mmus\Slc4a1

7 of 14

Mmus\Slc4a2

7 of 14

Mmus\Slc4a5

7 of 14

Yes

Mmus\Slc4a3

6 of 14

Mmus\Slc4a9

6 of 14

Yes

Mmus\Slc4a11

3 of 14

Yes

Xenopus tropicalis (Western clawed frog) (19)

Xtro\slc4a7

11 of 13

Yes

Xtro\slc4a8

7 of 13

Yes

Xtro\slc4a10

7 of 13

Yes

Xtro\slc4a4

5 of 13

Yes

Xtro\slc4a5

4 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

Yes

Xtro\slc4a11

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\sdhc

1 of 13

Xtro\tmem59

1 of 13

Yes

Danio rerio (Zebrafish) (14)

Drer\slc4a7

14 of 14

Yes

Drer\slc4a8

13 of 14

Yes

Drer\slc4a10b

13 of 14

Yes

Drer\slc4a10a

12 of 14

Yes

Drer\slc4a4a

9 of 14

Yes

Drer\slc4a4b

9 of 14

Yes

Drer\slc4a1b

7 of 14

Drer\slc4a2a

7 of 14

Drer\slc4a5a

7 of 14

Yes

6 of 14

6 of 14

6 of 14

2 of 14

Yes

Drer\slc4a11

2 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\abts-1

13 of 14

Yes

Cele\abts-2

5 of 14

Yes

Cele\abts-4

4 of 14

Cele\abts-3

2 of 14

Yes

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP009736

12 of 12

Yes

Agam\AgaP_AGAP006968

5 of 12

Agam\AgaP_AGAP006115

4 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\AT3G06450

6 of 13

Yes

Atha\AT3G62270

6 of 13

Yes

Atha\AT4G32510

6 of 13

Yes

Atha\AT5G25430

6 of 13

Yes

Atha\BOR1

6 of 13

Yes

Atha\BOR4

6 of 13

Yes

Atha\BOR5

6 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\BOR1

8 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC543.05c

8 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ndae1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Ae2

10 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SLC4A11; solute carrier family 4 member 11

model of Fuchs' endothelial dystrophy

IEA

(FlyBase, 2019-)

model of congenital hereditary endothelial dystrophy of cornea

IEA

(FlyBase, 2019-)

model of corneal dystrophy-perceptive deafness syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ndae1^GD967

exacerbates atypical teratoid rhabdoid tumor

modeled by Snr1^KK101602

(Tegeder et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC4A7; solute carrier family 4 member 7

13 of 14

603353

SLC4A8; solute carrier family 4 member 8

13 of 14

605024

SLC4A10; solute carrier family 4 member 10

13 of 14

605556

NEURODEVELOPMENTAL DISORDER WITH HYPOTONIA AND CHARACTERISTIC BRAIN ABNORMALITIES; NEDHBA

SLC4A4; solute carrier family 4 member 4

10 of 14

603345

PROXIMAL RENAL TUBULAR ACIDOSIS-OCULAR ANOMALY SYNDROME; PRTAO

SLC4A1; solute carrier family 4 member 1 (Diego blood group)

7 of 14

109270

hereditary spherocytosis type 4

SLC4A2; solute carrier family 4 member 2

7 of 14

109280

OSTEOPETROSIS, AUTOSOMAL RECESSIVE 9; OPTB9

SLC4A3; solute carrier family 4 member 3

7 of 14

106195

SHORT QT SYNDROME 7; SQT7

SLC4A5; solute carrier family 4 member 5

7 of 14

606757

SLC4A9; solute carrier family 4 member 9

7 of 14

610207

SLC4A11; solute carrier family 4 member 11

3 of 14

610206

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ndae1 - CG11836

experimental knowledge based

(Guruharsha et al., 2011)

Ndae1 - comt

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Snr1

suppressible

Ndae1

(Tegeder et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60151

DroID - A comprehensive database of gene and protein interactions.

FBgn0259111

MIST (protein-protein) - An integrated Molecular Interaction Database

34005

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-25

Cytogenetic map

27E5-27E7

Sequence location

2L:7,223,328..7,249,343 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

27E5-27E7

Limits computationally determined from genome sequence between P{PZ}snRNP70K⁰²¹⁰⁷ and P{PZ}wg⁰²⁶⁵⁷;

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

27E6-27E6

(Romero, 1998.2.6)

28A-28A

(determined by in situ hybridisation)

(Romero et al., 2000)

28C1-28C2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-25

(FlyBase, 2016)

2-23.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24330

w¹¹¹⁸; Mi{ET1}Ndae1^MB05294

65678

w¹¹¹⁸; PBac{IT.GAL4}Ndae1^1361-G4

38099

y¹ w^*; Mi{MIC}Ndae1^MI05100

55464

y¹ w^*; Mi{MIC}Ndae1^MI09906

30689

y¹ w^*; Mi{MIC}Ndae1^MI00281/CyO

14423

y¹ w^67c23; P{SUPor-P}Ndae1^KG02201

NIG-Fly

4675R-1

P{NIG.4675R}1

M2L-2078

y¹ w¹¹¹⁸; Ndae1^SK5 P{neoFRT}40A/CyO-Tb

v102707

v107948

v3664

v342880

P{hsFLP}1, y1 w1118; P{HD_CFD02408}attP40/CyO-GFP

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (29)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_HL01706

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259111

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259111

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG31906) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology.

(Roch et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ndae1 l(2)k10316

(Romero and Harte, 2000.10.11)

Source for merge of: Ndae1 CG31906

(FlyBase Genome Annotators, 2008)

Source for merge of: Ndae1 anon-WO0257455.13

(FlyBase, 1996-)

Additional comments

Annotations CG4675 and CG31906 merged as CG42253 in release 5.8 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Source for identity of Ndae1 CG4675 was sequence comparison ( date:000829 ).

(FlyBase, 1996-)

Source for merge of Ndae1 anon-WO0257455.13 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Ndae1 CG4675

(Romero et al., 2000, FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

153385_at

(Kristensen et al., 2005)

CG31906

(Keleman et al., 2009.8.5)

CG42253

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Sénatore et al., 2010, Perkins et al., 2009, FlyBase Genome Annotators, 2008)

CG4675

(Hirata et al., 2012, Khanna et al., 2010, Mosallanejad et al., 2010, Keleman et al., 2009.8.5, Perrin et al., 2004, Wang et al., 2004, Anholt and Mackay, 2001, Romero et al., 2000)

NDAE1

(Hirata et al., 2012, O'Donnell et al., 2003)

Ndae-1

(Capovilla et al., 2006)

anon-WO0257455.13

l(2)k10316

(Roch et al., 1998)

ndae1

(O'Farrell et al., 2023, Perrin et al., 2004)

Name Synonyms

CG31906

Na+-driven anion exchanger 1

(Tevy et al., 2014, Khanna et al., 2010, Qian and Bodmer, 2009, Romero et al., 2000)

Secondary FlyBase IDs

FBgn0031899
FBgn0021952
FBgn0063801
FBgn0051906

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34005

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IPI3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IPI3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259111

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34005/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259111

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259111

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42253

MARRVEL_MODEL - MARRVEL (model organism gene)

34005

Linkouts

BioGRID - A database of protein and genetic interactions.

60151

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0259111

DroID - A comprehensive database of gene and protein interactions.

FBgn0259111

DRSC - Results frm RNAi screens

FBgn0259111

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259111

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259111

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4675

Flygut - An atlas of the Drosophila adult midgut

FBgn0259111

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0259111

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033053

MIST (protein-protein) - An integrated Molecular Interaction Database

34005

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (86)

The following external sites may use different assemblies or annotations than FlyBase.

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