FlyBase Gene Report: Dmel\Fas1

General Information

Symbol

Dmel\Fas1

Species

D. melanogaster

Name

Fasciclin 1

Annotation Symbol

CG6588

Feature Type

protein_coding_gene

FlyBase ID

FBgn0285925

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Neural cell adhesion molecule. (UniProt, P10674)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

fasI, fasciclin I, MRE24, DROFASI, fas-I

Key Links

Genomic Location

Cytogenetic map

89D5-89D6

Sequence location

3R:16,625,238..16,639,771 [+]

Recombination map

3-59

RefSeq locus

NT_033777 REGION:16625238..16639771

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from direct assay

(Elkins et al., 1990)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from biological aspect of ancestor with PANTHER:PTN004207484

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in axon guidance

inferred from genetic interaction with FLYBASE:Abl; FB:FBgn0000017

(Elkins et al., 1990)

involved_in calcium-independent cell-cell adhesion

inferred from direct assay

(Elkins et al., 1990)

involved_in homophilic cell-cell adhesion

inferred from direct assay

(Elkins et al., 1990)

involved_in neuron recognition

inferred from direct assay

(Zinn et al., 1988)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in extracellular matrix organization

inferred from biological aspect of ancestor with PANTHER:PTN004207484

(GO Reference Genome Project, 2011-)

involved_in homophilic cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN004207484

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

inferred from direct assay

(Hortsch and Goodman, 1990)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN004207484

(GO Reference Genome Project, 2011-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN004207484

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Neural cell adhesion molecule.

(UniProt, P10674)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Fas1: Fasciclin I

Expression confined to neurons. In 12-14 h embryos high levels of fasciclin I are detectable in two commissural axon bundles (fascicles) per segment, a thick one in the posterior commissure and a thin one in the anterior commissure. In addition high expression is seen in two clusters of neuronal cell bodies per hemisegment as well as in the intersegmental and segmental nerve axons. Finally, fasciclin I is detected on the surface of all peripheral neurons. Postulated to be involved in neuron recognition during growth cone guidance. Flies deficient in FasI function are fully viable and CNS development apparently is normal.

Summary (Interactive Fly)

lipid linked novel protein - homophilic cell adhesion molecule that acts in axon guidance

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fas1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P10674)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

1O70

Comments on Gene Model

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Fas1-RA

FBtr0083332

NM_169726

3002

649

Fas1-RB

FBtr0083333

NM_169725

3011

652

Fas1-RC

FBtr0083334

NM_169724

3073

655

Fas1-RD

FBtr0112803

NM_001104344

3094

662

Fas1-RE

FBtr0300036

NM_001144593

3214

624

Fas1-RF

FBtr0300037

NM_001144594

3303

636

Fas1-RG

FBtr0333726

NM_001275716

4814

661

Additional Transcript Data and Comments

Reported size (kB)

5.2, 5.0, 4.2, 4.0, 3.2, 3.0 (northern blot)

(McAllister et al., 1992)

3.0 (longest cDNA)

(Zinn et al., 1988)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fas1-PA

FBpp0082782

72.2

649

6.91

FBpp0082783

72.6

652

7.03

FBpp0082784

73.0

655

7.03

FBpp0111715

73.7

662

7.53

FBpp0289313

69.5

624

6.91

FBpp0289314

70.9

636

6.63

FBpp0305873

73.6

661

6.63

A0A0B4KH94

NP_001262645

AGB96025

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

72 (kD observed)

(Hortsch and Goodman, 1990)

652 (aa); 70 (kD predicted)

(Zinn et al., 1988)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1O70

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fas1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.79

Transcript Expression

dissected tissue

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 15

larval ventral nerve cord

Comment: reference states 10-13 hr AEL

(McAllister et al., 1992)

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT21-0

(Xu et al., 2011)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

gonad

(McAllister et al., 1992)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 14

epidermis | dorsal

(McAllister et al., 1992)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

mesectoderm anlage | subset

(Kearney et al., 2004)

embryonic stage 9 -- 10

neuroblast | segmentally repeated

(McAllister et al., 1992)

ventral neurectoderm | segmentally repeated

(McAllister et al., 1992)

central brain primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 9 -- 17

organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 12 -- 13

ectoderm

(McAllister et al., 1992)

embryonic stage 12 -- 17

neuron of central nervous system | restricted

(McAllister et al., 1992)

embryonic stage 13 -- 15

larval ventral nerve cord

Comment: reference states 10-13 hr AEL

(McAllister et al., 1992)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval midline neuron

(Kearney et al., 2004)

dorsal median cell

(Kearney et al., 2004)

embryonic stage 13 -- 17

presumptive embryonic/larval peripheral nervous system

(McAllister et al., 1992)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(McAllister et al., 1992)

larval stage

(McAllister et al., 1992)

third instar larval stage

(McAllister et al., 1992)

adult stage

(McAllister et al., 1992)

oogenesis

(McAllister et al., 1992)

Additional Descriptive Data

Zygotic-specific isoforms of Fas1 with long 3' UTR extensions were observed.

(Hilgers et al., 2011)

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval spiracle

(McAllister et al., 1992)

salivary gland body primordium

(McAllister et al., 1992)

inclusive hindgut primordium

(McAllister et al., 1992)

posterior midgut primordium

(McAllister et al., 1992)

gonad

(McAllister et al., 1992)

embryonic stage 1 -- 14

epidermis | dorsal

(McAllister et al., 1992)

embryonic stage 9 -- 10

neuroblast | segmentally repeated

(McAllister et al., 1992)

ventral neurectoderm | segmentally repeated

(McAllister et al., 1992)

embryonic stage 12

larval VUM neuron

(McAllister et al., 1992)

embryonic stage 12 -- 13

ectoderm

(McAllister et al., 1992)

embryonic stage 12 -- 16

larval DA1 motor neuron

(McAllister et al., 1992)

larval VO2 motor neuron

(McAllister et al., 1992)

embryonic stage 12 -- 17

neuron of central nervous system | restricted

(McAllister et al., 1992)

embryonic stage 13 -- 16

terminal organ

Comment: 11-14 hr AEL

(Zinn et al., 1988)

embryonic stage 13 -- 17

presumptive embryonic/larval peripheral nervous system

(McAllister et al., 1992)

symmetrical commissure

(McAllister et al., 1992)

embryonic stage 14 -- 16

larval anterior commissure

Comment: reference states 11-14 hr AEL

(Zinn et al., 1988)

larval posterior commissure

Comment: reference states 11-14 hr AEL

(Zinn et al., 1988)

larval VO2 motor neuron

• neuronal cell body & axon

(Zinn et al., 1988)

larval segmental nerve

• axon

(Zinn et al., 1988)

larval DA1 motor neuron

• neuronal cell body & axon

(Zinn et al., 1988)

antennal sense organ

Comment: 11-14 hr AEL

(Zinn et al., 1988)

larval intersegmental nerve

• axon

(Zinn et al., 1988)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

inferred from direct assay

(Hortsch and Goodman, 1990)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fas1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fas1

View exonic expression by tissue for Dmel\Fas1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42025/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0285925

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000634

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0285925

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6588

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 22 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fas1

Transgenic constructs containing regulatory region of Fas1

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel6270

(Ryder, 2004.4.26)

Df(3R)Ubx109

(Zinn et al., 1988)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Fas1^EY06622

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Fas1^GD12817

viable, with Scer\GAL4^Mef2.PR

Fas1^GD12817

viable, with Scer\GAL4^pnr-MD237

Fas1^GD12817

viable, with Scer\GAL4^tin.CΔ4

Fas1^GD12817

Sterility

Allele

fertile

Fas1^EY06622

Other Phenotypes

Allele

abnormal behavior | recessive

Fas1^TE77

abnormal grooming behavior

Fas1^TE77

abnormal neuroanatomy

abnormal neuroanatomy, with Scer\GAL4^MS1075

Fas1^+3.UAS

abnormal neurophysiology

visible, with Scer\GAL4^Bx-MS1096

Fas1^KK109572

wild-type

Fas1^+t23

Phenotype manifest in

Allele

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Fas1^EY06622

ocellar nerve, with Scer\GAL4^MS1075

Fas1^+3.UAS

wing, with Scer\GAL4^Bx-MS1096

Fas1^KK109572

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\TGFBI

4 of 14

Yes

Hsap\POSTN

3 of 14

Hsap\STAB2

2 of 14

Yes

Hsap\STAB1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Tgfbi

4 of 14

Yes

Rnor\Postn

3 of 14

Rnor\Stab1

1 of 14

Rnor\Stab2

1 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Tgfbi

4 of 14

Yes

Mmus\Postn

2 of 14

Mmus\Stab1

1 of 14

Mmus\Stab2

1 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\tgfbi

3 of 13

Yes

Xtro\stab2

2 of 13

Yes

Xtro\phox2a

1 of 13

Xtro\postn

1 of 13

Xtro\stab1

1 of 13

Yes

Danio rerio (Zebrafish) (5)

Drer\tgfbi

4 of 14

Yes

Drer\postna

3 of 14

Drer\postnb

2 of 14

Drer\stab2

2 of 14

Yes

Drer\stab1

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\F26E4.7

8 of 14

Yes

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP000968

11 of 12

Yes

Agam\AgaP_AGAP004428

2 of 12

Agam\AgaP_AGAP010168

2 of 12

Agam\AgaP_AGAP010238

1 of 12

Arabidopsis thaliana (thale-cress) (5)

Atha\AT5G05650

1 of 13

Yes

Atha\FLA15

1 of 13

Yes

Atha\FLA16

1 of 13

Yes

Atha\FLA17

1 of 13

Yes

Atha\FLA18

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YLR001C

3 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC22H12.05c

3 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fas1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

CG43333

5 of 13

mfas

5 of 13

CG5758

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

TGFBI; transforming growth factor beta induced

model of Thiel-Behnke corneal dystrophy

IEA

(FlyBase, 2019-)

model of epithelial basement membrane dystrophy

IEA

(FlyBase, 2019-)

model of lattice corneal dystrophy

IEA

(FlyBase, 2019-)

model of granular corneal dystrophy 1

IEA

(FlyBase, 2019-)

model of Reis-Bucklers corneal dystrophy

IEA

(FlyBase, 2019-)

model of granular corneal dystrophy 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TGFBI; transforming growth factor beta induced

4 of 14

601692

POSTN; periostin

3 of 14

608777

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Fas1

bead aggregation assay, fluorescence microscopy, inferred by author

(Kania et al., 1993)

Fas1 - apolpp

cosedimentation, western blot

(Panakova et al., 2005)

RNA-protein

Physical Interaction

Assay

References

Fas1 - elav

anti bait coimmunoprecipitation, primer specific pcr

(Hilgers et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bif

enhanceable

Fas1

(Bahri et al., 1997)

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

(Shigenobu et al., 2006)

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-59

Cytogenetic map

89D5-89D6

Sequence location

3R:16,625,238..16,639,771 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89D5-89D6

Limits computationally determined from genome sequence between P{lacW}CSN5^L4032 and P{EP}MESK4^EP1015

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89E1-89E2

(determined by in situ hybridisation) 89D (determined by in situ hybridisation)

(Zinn et al., 1988)

Experimentally Determined Recombination Data

Location

3-59

(FlyBase, 2016)

3-58.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

43380

w^*; P{GSV1}Fas1^C607

62666

w¹¹¹⁸; PBac{IT.GAL4}Fas1^0143-G4

603474

y¹ w¹; P{TKO-UAS.GS06232}attP40/CyO

603475

y¹ w¹; P{TKO-UAS.GS06233}attP40/CyO

19855

y¹ w^67c23; P{EPgy2}Fas1^EY06622

41854

y¹ v¹; P{TRiP.GL01282}attP2/TM3, Sb¹

62577

w¹¹¹⁸; PBac{IT.GAL4}Fas1^0015-G4/TM6B, Tb¹

42887

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02580}attP40

6588R-1

v101779

v23014

w¹¹¹⁸; P{GD12817}v23014

v23015

w¹¹¹⁸; P{GD12817}v23015

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (223)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI05280

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Hortsch and Goodman, 1990)

polyclonal

(Zinn et al., 1988)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

F5H7_anti-Fasciclin_I

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : Stable cell lines were created containing Nrg constructs. They are designated S2:pRmHa3-nrg [167] (short isoform), S2:pRmHa3-nrg [180] (long isoform), and S2:pRmHa3-nrg [GPI] (containing the first 1121 amino acids of Nrg fused to the 53 C-terminal amino acids of Fas1 containing the GPI attachment signal.
(Hortsch et al., 1995)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

Area matching Fas1, Acc. No. M32311.

(Davies, 2001.3.15)

The complete sequence of the bithorax complex is available.

(Martin et al., 1995)

The phenotypes of Fas1, Fas2, Fas3 and nac mutants were analysed in the developing wing: the axon tracts in the CNS for the most part remain unaltered, and none of the phenotypes are 100% penetrant, indicating a fine-tuning role for these molecules in both the PNS and CNS.

(Whitlock, 1993)

Identification: Isolated from Drosophila embryonic cDNA libraries using a fasciclin I probe from grasshopper.

(Lindsley and Zimm, 1992)

Flies deficient in Fas1 function are fully viable and CNS development apparently is normal.

(Lindsley and Zimm, 1992)

Postulated to be involved in neuron recognition during growth cone guidance.

(Lindsley and Zimm, 1992)

Detailed molecular analysis of Fas1 reveals alternative splicing of the 6-9 base pair micro-exons.

(McAllister et al., 1992)

Fas1 plays an important role in growth cone guidance, as revealed in an Abl mutant background.

(Elkins et al., 1990)

Mutant phenotypes can be rescued by element mediated transformation of a wild type copy of Fas1.

(Elkins et al., 1990)

A screening procedure was used to recover second-site mutations that would shift the lethal phase of Abl mutant homozygotes and Nrt mutant heterozygotes from embryonic or larval stages back to pupal. ena mutations allow complete recovery of Abl^- Fas1^- double mutants as fertile adults.

(Gertler et al., 1990)

Fas1 protein is tightly associated with the lipid bilayer in Schneider 1 cells. In Drosophila embryos a significant amount of Fas1 protein is present in a free, soluble form, the ration of the free and membrane bound forms varying considerably during embryogenesis.

(Hortsch and Goodman, 1990)

Study of the expression pattern supports the hypothesis that Fas1 is a neuronal surface recognition molecule.

(Zinn et al., 1988)

Relationship to Other Genes

Source for database merge of

Source for merge of: Fas1 MRE24

(FlyBase Genome Annotators, 2011)

Source for merge of: Fas1 FGM222E05

(FlyBase, 1996-)

Additional comments

MRE24 is part of the 3' UTR of Fas1.

(FlyBase Genome Annotators, 2011)

MRE24 corresponds to a portion of the 3' UTR of Fas1.

(FlyBase Genome Annotators, 2011)

FGM222E05 corresponds to the 3' UTR of Fas1; merged in FB2017_02.

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG6588

(Jensen et al., 2013, Jaspers et al., 2012, Lim et al., 2011, Khanna et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Schiess et al., 2009, Molnar et al., 2006)

DROFAS

(Schug et al., 1998)

DROFAS1

(Schug et al., 1997)

DROFASI

(Fernando Vazquez et al., 2000, Lopez et al., 1999)

FGM222E05

(Shigenobu et al., 2006)

Fas

(Liu et al., 2025)

Fas I

(Liu and Seeger, 2000)

FasI

(Bornstein et al., 2015, Koles et al., 2012, Yamamoto-Hino et al., 2010, Choksi et al., 2006, Bender and Hudson, 2000, Van Etten, 1999, Speicher et al., 1998, Zhong and Shanley, 1995, Desai et al., 1994, Kania et al., 1993)

MRE24

(FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)

fas I

(Gertler et al., 1990)

fas-I

(Jimenez et al., 1993, Pulido, 1993)

fas1

(Hilgers et al., 2012, Smibert et al., 2012, Hilgers et al., 2011)

fasI

(Garcia-Alonso, 1999, Chien, 1998, Bahri et al., 1997, Budnik, 1996, Chien, 1996, Hamilton et al., 1995, Therianos et al., 1995, Broadie et al., 1993, Nambu et al., 1993, Noll et al., 1993, Phillis et al., 1993, Wang et al., 1993, Whitlock, 1993, McAllister et al., 1992, McAllister et al., 1992, Goodman et al., 1989)

gasI

(Palka, 1993)

Name Synonyms

Fasciclin

(Liu et al., 2024)

Fasciclin 1

(Kwong et al., 2015, Jensen et al., 2013, Jaspers et al., 2012, Lim et al., 2011, Khanna et al., 2010, Khanna et al., 2010, Tan et al., 2009, Stofanko et al., 2008)

Fasciclin I

(Bornstein et al., 2015, Koles et al., 2012, Southall and Brand, 2009, Choksi et al., 2006, Panakova et al., 2005, Goodman and Doe, 1993)

Fasciclin-1

(Hoehne et al., 2005)

fasciclin I

(Burghes et al., 2001, Malhotra et al., 1998, Seeger, 1994, Therianos et al., 1994, Wilson, 1993, Grenningloh and Goodman, 1992, McAllister et al., 1992, Hortsch and Bieber, 1991, Elkins et al., 1990, Hortsch and Goodman, 1990)

mRNA-like ncRNA in embryogenesis 24

Secondary FlyBase IDs

FBgn0262742
FBgn0000634
FBgn0026839
FBgn0086555
FBgn0086344

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 64 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42025

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P10674

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P10674

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P10674

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42025/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0285925

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0285925

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6588

MARRVEL_MODEL - MARRVEL (model organism gene)

42025

PDB - An information portal to biological macromolecular structures

1O70

Linkouts

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

F5H7_anti-Fasciclin_I

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000634

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0285925

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6588

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (164)

The following external sites may use different assemblies or annotations than FlyBase.

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